FlyBase Allele Report: Dmel\sc[UAS.cCa]

General Information

Symbol

Dmel\sc^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chien

FlyBase ID

FBal0056210

Feature type

allele

Associated gene

Dmel\sc

Associated Insertion(s)

Carried in Construct

P{UAS-sc.C}

Also Known As

UAS-sc

Key Links

Transgenic product class

wild_type

(Chien et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chien et al., 1996)

Mutagen

in vitro construct

(Chien et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-sc.C}

Cytology

Description

sc is expressed under the control of UAS regulatory sequences.

(Chien et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive, with Scer\GAL4^hs.PB

(Huang et al., 2000)

lethal - all die before end of P-stage, with Scer\GAL4^ptc-559.1

(Jarman and Ahmed, 1998)

some die during pharate adult stage, with Scer\GAL4^ptc-559.1

(Jarman and Ahmed, 1998)

visible, with Scer\GAL4¹⁰⁹

(Jarman and Ahmed, 1998)

visible, with Scer\GAL4^hs.PB

(Chien et al., 1996)

visible, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

visible, with Scer\GAL4^ptc-559.1

(Jarman and Ahmed, 1998)

visible, with Scer\GAL4^sca-109-68

(Powell et al., 2012)

visible | heat sensitive, with Scer\GAL4^hs.PB

(Huang et al., 2000)

Phenotype Manifest In

macrochaeta, with Scer\GAL4¹⁰⁹

(Jarman and Ahmed, 1998)

macrochaeta | ectopic, with Scer\GAL4^hs.PB

(Chien et al., 1996)

multidendritic neuron | ectopic, with Scer\GAL4^sca-537.4

(Huang et al., 2000)

scutellar bristle, with Scer\GAL4¹⁰⁹

(Jarman and Ahmed, 1998)

scutellar bristle | ectopic, with Scer\GAL4^sca-109-68

(Powell et al., 2012, Rawlins et al., 2003)

scutellum & microchaeta, with Scer\GAL4¹⁰⁹

(Jarman and Ahmed, 1998)

scutum, with Scer\GAL4¹⁰⁹

(Jarman and Ahmed, 1998)

sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4¹⁰⁹

(Jarman and Ahmed, 1998)

sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4^ptc-559.1

(Jarman and Ahmed, 1998)

wing, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

wing, with Scer\GAL4^ptc-559.1

(Jarman and Ahmed, 1998)

wing sensillum | ectopic, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

wing sensillum | ectopic | heat sensitive, with Scer\GAL4^hs.PB

(Huang et al., 2000)

wing vein L2, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

wing vein L3, with Scer\GAL4¹⁰⁹

(Jarman and Ahmed, 1998)

Detailed Description

Statement

Reference

Expression of sc^Scer\UAS.cCa under the control of Scer\GAL4^sca-109-68 results in ectopic scutellar bristles.

(Powell et al., 2012)

Expression of sc^Scer\UAS.cCa under the control of Scer\GAL4^kni.L2 results in L2 wing veins which are covered in ectopic bristles. In addition, ectopic bristles form sporadically in a broad posterior domain of the wing.

(Lunde et al., 2003)

sc^Scer\UAS.cCa; Scer\GAL4^sca-109-68 flies have extra scutellar bristles.

(Rawlins et al., 2003)

Expression of sc^Scer\UAS.cCa under the control of Scer\GAL4^sca-537.4 slightly induces the formation of multiple dendritic neurons in embryos. Few ectopic chordotonal neurons are induced in these embryos. Expression of sc^Scer\UAS.cCa under the control of Scer\GAL4^hs.PB using a 5 minute heat shock just after puparium formation results in ectopic bristles in the wing.

(Huang et al., 2000)

Flies expressing sc^Scer\UAS.cCa under the control of Scer\GAL4¹⁰⁹ have extra bristles in defined areas of the cuticle. The phenotype is strongest on the scutellum (where extra macrochaetae and microchaetae are formed) and the third wing vein, and to a lesser degree on the notum. The phenotype increases with temperature. No ectopic chordotonal organs are formed. Extra sensory organ precursors are seen in the wing discs of flies expressing sc^Scer\UAS.cCa under the control of Scer\GAL4¹⁰⁹. Flies expressing sc^Scer\UAS.cCa under the control of Scer\GAL4^ptc-559.1 are not viable and die as pharate adults with deformed wings and appendages. Ectopic sensory organ precursors are seen in the wing discs of these animals.

(Jarman and Ahmed, 1998)

Produces an abundance of ectopic single bristles that are evenly distributed over the wing, when expressed using Scer\GAL4^hs.PB. Ectopic chordotonal organs in the wing are not produced in these flies.

(Chien et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sca-109-68, sc^UAS.cCa has visible phenotype, enhanceable by Sumo^{UASp.Tag:FLAG.Tag:polyHis}/Scer\GAL4^sca-109-68

(Powell et al., 2012)

Suppressor of

Statement

Reference

sc^UAS.cCa/Scer\GAL4⁷ is a suppressor of visible phenotype of ato¹

(Sun et al., 2000)

Other

Statement

Reference

Scer\GAL4⁷, ato¹, sc^UAS.cCa has visible phenotype

(Sun et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sca-109-68, sc^UAS.cCa has scutellar bristle | ectopic phenotype, enhanceable by Sumo^{UASp.Tag:FLAG.Tag:polyHis}/Scer\GAL4^sca-109-68

(Powell et al., 2012)

Scer\GAL4^sca-109-68, sc^UAS.cCa has scutellar bristle | ectopic phenotype, enhanceable by ed^4.12

(Rawlins et al., 2003)

Suppressor of

Statement

Reference

sc^UAS.cCa/Scer\GAL4⁷ is a suppressor of ommatidium phenotype of ato¹

(Sun et al., 2003, Sun et al., 2000)

sc^UAS.cCa/Scer\GAL4⁷ is a suppressor of eye phenotype of ato¹

(Sun et al., 2000)

NOT Suppressor of

Statement

Reference

sc^UAS.cCa/Scer\GAL4⁷ is a non-suppressor of photoreceptor cell R8 phenotype of ato¹

(Sun et al., 2000)

Other

Statement

Reference

Scer\GAL4⁷, ato¹, sc^UAS.cCa has interommatidial bristle phenotype

(Sun et al., 2003)

Scer\GAL4⁷, ato¹, sc^UAS.cCa has macrochaeta | ectopic phenotype

(Sun et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of smt3^{Scer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6} enhances the formation of ectopic scutellar bristles caused by expression of sc^Scer\UAS.cCa under the control of Scer\GAL4^sca-109-68.

(Powell et al., 2012)

Expression of sc^Scer\UAS.cCa under the control of Scer\GAL4^Eq1 restores external sensory organ formation in the notum of Df(1)sc10-1 flies.

(Pi et al., 2004)

Expression of sc^Scer\UAS.cCa under the control of Scer\GAL4⁷ in ato¹ flies rescues a small number of ommatidia. The eyes contain numerous interommatidial bristles in these flies.

(Sun et al., 2003)

The expression of sc^Scer\UAS.cCa when driven by Scer\GAL4⁷ in ato¹ flies does result in partial restoration of the compound eye. Ommatidia contain only 2-5 photoreceptors, usually lacking the centrally located R8 photoreceptors with small rhabdomeres. Occasionally bristles that resemble macrochaetae are seen in various positions in the eye.

(Sun et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sc^Scer\UAS.cCa

sc^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Chien

(Chien et al., 1996)

Secondary FlyBase IDs

References (12)

Report Sections

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