FlyBase Allele Report: Dmel\kuz[DN.UAS]

General Information

Symbol

Dmel\kuz^DN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0062773

Feature type

allele

Associated gene

Dmel\kuz

Associated Insertion(s)

Carried in Construct

P{UAS-kuz.DN}

Also Known As

UAS-kuz^DN, UAS-KUZ-DN, kuzDN

Key Links

Transgenic product class

dominant_negative_variant

(Pan and Rubin, 1997)

inframe_deletion

(Pan and Rubin, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Pan and Rubin, 1997)

inframe_deletion

(Pan and Rubin, 1997)

Mutagen

in vitro construct

(Pan and Rubin, 1997)

Progenitor genotype

Carried in construct

P{UAS-kuz.DN}

Cytology

Description

UASt regulatory sequences drive expression of a dominant-negative form of kuz. The Pro-domain and metalloprotease domain are deleted, leaving the SP, disintegrin domain, Cys-rich domain, transmembrane domain and intracellular domain intact.

(Pan and Rubin, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

kuz

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

ameliorates brain cancer

modeled by Vps35^E42

(Li et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^repo

(Edenfeld et al., 2007)

abnormal neuroanatomy | embryonic stage 16, with Scer\GAL4^ap-md544

(Coleman et al., 2010)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Wang et al., 2006)

border follicle cell | heat sensitive, with Scer\GAL4^slbo.2.6

(Wang et al., 2007)

commissure | embryonic stage 16, with Scer\GAL4^ap-md544

(Coleman et al., 2010)

dMP2 neuron | ectopic | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

embryonic dorsal vessel, with Scer\GAL4^twi.PG

(Albrecht et al., 2006)

embryonic heart cardioblast | increased number, with Scer\GAL4^twi.PG

(Albrecht et al., 2006)

embryonic pericardial cell, with Scer\GAL4^twi.PG

(Albrecht et al., 2006)

embryonic somatic muscle cell, with Scer\GAL4^sim.PS

(Schimmelpfeng et al., 2001)

embryonic somatic muscle cell, with Scer\GAL4^twi.PG

(Albrecht et al., 2006)

eye, with Scer\GAL4^32B

(Qi et al., 1999)

larval DA1 motor neuron | ectopic | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

larval longitudinal connective, with Scer\GAL4^elav-C155

(Pan and Rubin, 1997)

larval longitudinal connective, with Scer\GAL4^sim.PS

(Schimmelpfeng et al., 2001)

larval longitudinal connective | embryonic stage 16, with Scer\GAL4^ap-md544

(Coleman et al., 2010)

larval RP2 motor neuron | ectopic | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

neuroblast MP2 | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

neuroblast NB1-1 | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

neuroblast NB4-2 | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

pCC neuron | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

PNS glial cell, with Scer\GAL4^repo

(Edenfeld et al., 2007)

presumptive embryonic/larval central nervous system, with Scer\GAL4^sim.PS

(Schimmelpfeng et al., 2001)

RP2sib neuron | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

scutum & macrochaeta, with Scer\GAL4^32B

(Qi et al., 1999)

subperineurial glial cell | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

vMP2 neuron | somatic clone, with Scer\GAL4^sca-109-68

(Udolph et al., 2009)

wing, with Scer\GAL4^32B

(Qi et al., 1999)

wing vein, with Scer\GAL4^32B

(Qi et al., 1999)

Detailed Description

Statement

Reference

Expression of kuz^DN.Scer\UAS in the ap ipsilateral neurons under the control of Scer\GAL4^ap-md544 causes ap neurons to ectopically cross the midline in approximately 15% of segments in stage 16 embryos.

(Coleman et al., 2010)

Expression of kuz^DN.Scer\UAS under the control of Scer\GAL4^sca-109-68 results in duplication of the RP2 neurons.

Implantation of cells from Scer\GAL4^sca-109-68 :kuz^DN.Scer\UAS mutant embryos into wild-type embryos results in duplication of the aCC neuron at the expense of the pCC neuron as well as a loss of subperineural glia in NB1-1 lineages, duplication of the RP2 neuron at the expense of the RP2 sib as well as absence of the additional motor neurons in the NB4-2 lineage, duplication of the dMP2 cell in the MP2 lineage, and a complete absence of the U-neurons in the NB7-1 lineage.

(Udolph et al., 2009)

Expression of kuz^DN.Scer\UAS under the control of Scer\GAL4^repo results in irregular peripheral glial cell migration in embryos.

(Edenfeld et al., 2007)

Expression of kuz^DN.Scer\UAS in border cells (under the control of Scer\GAL4^slbo.2.6) suppresses border cell migration. This phenotype appears stronger at 29[o]C. The morphology of the border cells clusters is frequently different from wild-type. The clusters appear less round and the cells less tightly clustered.

(Wang et al., 2007)

Expression of kuz^DN.Scer\UAS driven by Scer\GAL4^twi.PG results in a hyperplasic heart, with the number of cardioblasts being increased approximately twofold. The number of pericardial cells appears to be reduced. The embryos also show severe muscle malformation defects.

(Albrecht et al., 2006)

Expression of kuz^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 results in a severe impairment of border follicle cell migration. More than 90% of stage 10 egg chambers show delayed border cell migration, and border cells remain at the anterior tip of the egg chamber in 45% of cases.

(Wang et al., 2006)

Expression of kuz^DN.Scer\UAS under the control of Scer\GAL4^sim.PS results in no gross central nervous system (CNS) defects in 18% of embryos, while the remaining embryos have Fas2-positive axons crossing the midline. 40% of these embryos have a robo-like axon phenotype. The formation of the lateral Fas2-positive fascicle is affected. Muscle fibres are seen to cross the CNS dorsally.

(Schimmelpfeng et al., 2001)

When expression is driven by Scer\GAL4^32B, extra wing vein material develops in the wing, most notably forming deltas where the vein reaches the wing margin, the eyes are small and rough and extra bristles form on the notum.

(Qi et al., 1999)

Scer\GAL4^elav-C155-mediated expression cause major defects in axonal pathways, such as disruption of longitudinal axonal tracts.

(Pan and Rubin, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, kuz^DN.UAS has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by robo[+]/robo1^unspecified/Scer\GAL4^ap-md544

(Coleman et al., 2010)

Scer\GAL4^ap-md544, kuz^DN.UAS has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by sli²/sli[+]

(Coleman et al., 2010)

Enhancer of

Statement

Reference

kuz^DN.UAS, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Scer\GAL4^ap-md544/kuz^DN.UAS is an enhancer of abnormal neuroanatomy | embryonic stage 16 | dominant phenotype of robo1^unspecified

(Coleman et al., 2010)

Scer\GAL4^ap-md544/kuz^DN.UAS is an enhancer of abnormal neuroanatomy | embryonic stage 16 | dominant phenotype of sli²

(Coleman et al., 2010)

Suppressor of

Statement

Reference

kuz^DN.UAS/Scer\GAL4^pnt-14-94 is a suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

kuz^DN.UAS/Scer\GAL4^GMR9D11 is a suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

kuz^DN.UAS/Scer\GAL4^pnt-14-94 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

kuz^DN.UAS/Scer\GAL4^GMR9D11 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, kuz^DN.UAS has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by sli²/Scer\GAL4^ap-md544/sli[+]

(Coleman et al., 2010)

Scer\GAL4^ap-md544, kuz^DN.UAS has commissure | embryonic stage 16 phenotype, enhanceable by sli²/Scer\GAL4^ap-md544/sli[+]

(Coleman et al., 2010)

Scer\GAL4^ap-md544, kuz^DN.UAS has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by robo[+]/robo1^unspecified/Scer\GAL4^ap-md544

(Coleman et al., 2010)

Scer\GAL4^ap-md544, kuz^DN.UAS has commissure | embryonic stage 16 phenotype, enhanceable by robo[+]/robo1^unspecified/Scer\GAL4^ap-md544

(Coleman et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^slbo.2.6, kuz^DN.UAS has border follicle cell | heat sensitive phenotype, suppressible by N^int.G.UAS, Scer\GAL4^slbo.2.6

(Wang et al., 2007)

NOT suppressed by

Statement

Reference

Scer\GAL4^32B, kuz^DN.UAS has wing phenotype, non-suppressible by N^+tCos479

(Qi et al., 1999)

Scer\GAL4^32B, kuz^DN.UAS has wing vein phenotype, non-suppressible by N^+tCos479

(Qi et al., 1999)

Scer\GAL4^32B, kuz^DN.UAS has eye phenotype, non-suppressible by N^+tCos479

(Qi et al., 1999)

Scer\GAL4^32B, kuz^DN.UAS has scutum & macrochaeta phenotype, non-suppressible by N^+tCos479

(Qi et al., 1999)

Enhancer of

Statement

Reference

kuz^DN.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Scer\GAL4^ap-md544/kuz^DN.UAS is an enhancer of larval longitudinal connective | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

Scer\GAL4^ap-md544/kuz^DN.UAS is an enhancer of commissure | embryonic stage 16 phenotype of robo1^unspecified

(Coleman et al., 2010)

Scer\GAL4^ap-md544/kuz^DN.UAS is an enhancer of larval longitudinal connective | embryonic stage 16 phenotype of sli²

(Coleman et al., 2010)

Scer\GAL4^ap-md544/kuz^DN.UAS is an enhancer of commissure | embryonic stage 16 phenotype of sli²

(Coleman et al., 2010)

Suppressor of

Statement

Reference

kuz^DN.UAS/Scer\GAL4^GMR9D11 is a suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

kuz^DN.UAS/Scer\GAL4^pnt-14-94 is a suppressor of embryonic/larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

kuz^DN.UAS/Scer\GAL4^GMR9D11 is a suppressor of embryonic/larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

kuz^DN.UAS/Scer\GAL4^pnt-14-94 is a suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of kuz^DN.Scer\UAS in ipsilateral ap neurons under the control of Scer\GAL4^ap-md544 in a sli²/+ mutant background produces more severe ap axon midline crossing defects in stage 16 embryos than in either mutant alone.

One copy of robo^unspecified enhances the ectopic midline crossing phenotype seen when kuz^DN.Scer\UAS is expressed in the ipsilateral ap neurons of stage 16 embryos under the control of Scer\GAL4^ap-md544.

Expression of kuz^DN.Scer\UAS in the ipsilateral ap neurons under the control of Scer\GAL4^ap-md544 enhances the ectopic midline crossing phenotype seen in robo^unspecified/+ stage 16 embryos.

(Coleman et al., 2010)

Co-expression of N^{int.G.Scer\UAS} with kuz^DN.Scer\UAS in egg chamber border cells (under the control of Scer\GAL4^slbo.2.6) ameliorates the migration defect observed in kuz^DN.Scer\UAS mutants.

(Wang et al., 2007)

The phenotypes caused by expression of kuz^DN.Scer\UAS driven by Scer\GAL4^32B are suppressed by three copies of wild type Dl, but not by N^+tCos479.

(Qi et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

6578

108838

Notes on Origin

Discoverer

Comments

Protein functions in a dominant negative manner.

(Pan and Rubin, 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

kuz^DN.Scer\UAS

kuz^DN.UAS

kuz^DN

(Edenfeld et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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