FlyBase Allele Report: Dmel\aos[UAS.cHa]

Overexpression of argos^Scer\UAS.cHa, under the control of Scer\GAL4^en-e16E in the posterior compartment of the wing leads to 100% of vein loss in vein L4, and 90% in vein L5.

(Marenda et al., 2006)

Expression of argos^Scer\UAS.cHa, under the control of Scer\GAL4^en-e16E, in embryonic posterior compartment cells leads to induction of apoptosis and reduction in compartment cell number. Surviving cells are larger than normal.

(Parker, 2006)

Expression of argos^Scer\UAS.cHa, under the control of Scer\GAL4^hs.2sev results in a decrease in cone, 2^o, and 3^o cell numbers in the eye.

(Wildonger et al., 2005)

Stage 16 argos^Scer\UAS.cHa; Scer\GAL4^en-e16E embryos have reduced numbers of oenocytes per cluster. Most clusters have 3 oenocytes, but a few have the wild-type 6, suggesting that one round of delamination of 3 oenocytes occurs as in wild-type but that a second round is frequently blocked.

(Brodu et al., 2004)

Expression of Scer\GAL4^69B under the control of Scer\GAL4^69B results in a vein loss phenotype in the wing.

(Donaldson et al., 2004)

Expression of aos^Scer\UAS.cHa under the control of Scer\GAL4^69B results in a narrower wing in which vein L4 and the anterior crossvein are missing.

(MacDougall et al., 2004)

When argos^Scer\UAS.cHa is driven by Scer\GAL4^sca-537.4 27% of bracts in the tibia and 85% in the basitarsus of the second leg remain. Animals have fewer bracts in dorsal vs ventral rows on the basitarsus. The dorsal rows 4 and 5 have 27% and 44% of bracts respectively, compared to 85-100% for the remaining rows. Also in 3/10 legs a transformation of the huge 'apical' bristle into a chemosensory bristle of ordinary size is seen. When driven by Scer\GAL4^Dll-md23 26% of bracts in the tibia and 30% in the basitarsus of the second leg remain. Animals have fewer bracts in dorsal vs ventral rows on the basitarsus of the second leg. Mutant legs also lack claws and apodemes. Also in 4/10 legs a transformation of the huge 'apical' bristle into a chemosensory bristle of ordinary size is seen.

(Held, 2002)

Clones of argos^Scer\UAS.cHa (driven by Scer\GAL4^αTub84B.PC) in the adult abdomen do not show any consistent alterations of normal polarity.

(Lawrence et al., 2002)

When argos^Scer\UAS.cHa is driven by Scer\GAL4^sca-537.4 all bracts are removed.

(del Alamo et al., 2002)

When expression is driven by Scer\GAL4^sca-C253, both macrochaetae and microchatea are suppressed on the notum.

(Culi et al., 2001)

Expression of argos^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 has no effect on furrow initiation in the eye disc.

(Kumar and Moses, 2001)

Wing veins develop normally when argos^Scer\UAS.cHa is expressed under the control of Scer\GAL4⁶⁰⁴ and in some cases ectopic wing vein tissue is formed, in close proximity to vein territories.

(Martin-Blanco et al., 1999)

Expression of argos^Scer\UAS.cHa by Scer\GAL4^71B produces wings with a vein loss phenotype.

(Wessells et al., 1999)

Expression of argos^Scer\UAS.cHa in the developing wing blade using Scer\GAL4^Bx-MS1096 causes the loss of most wing veins.

(Howes et al., 1998)

Scer\GAL4^69B-mediated expression causes a mild or moderate extra-vein phenotype. Winga ares also smaller than wild type as vein cells that replace inter vein cells are more compact. Mediated expression also causes a rough eye phenotype: eyes are smaller than wild type and some ommatidia are fused. Scer\GAL4^bs-1348-mediated expression causes a mild or moderate extra-vein phenotype.

(Schnepp et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^69B, aos^UAS.cHa has visible phenotype, enhanceable by Pi3K68D^UAS.Tag:MYC, Scer\GAL4^69B

(MacDougall et al., 2004)

NOT Enhanced by

Statement

Reference

Scer\GAL4^69B, aos^UAS.cHa has visible phenotype, non-enhanceable by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, aos^UAS.cHa has visible phenotype, suppressible | partially by rho-5^KO1

(Zettl et al., 2011)

Scer\GAL4^GMR.PF, aos^UAS.cHa has visible phenotype, suppressible | partially by sip3^GD3145/Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Scer\GAL4^GMR.PF, aos^UAS.cHa has visible phenotype, suppressible | partially by Edem2^RNAi.UAS, Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Scer\GAL4^69B, aos^UAS.cHa has visible phenotype, suppressible by Df(3L)vin3/+

(MacDougall et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^69B, aos^UAS.cHa has visible phenotype, non-suppressible by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Suppressor of

Statement

Reference

aos^UAS.cHa/Scer\GAL4^69B is a suppressor of visible phenotype of Scer\GAL4^69B, vn^ΔIg.UAS

(Donaldson et al., 2004)

aos^UAS.cHa, Scer\GAL4^sca-C253 is a suppressor of visible phenotype of Delta^DN.UAS, Scer\GAL4^sca-C253

(Culi et al., 2001)

Other

Statement

Reference

Delta^DN.UAS, Scer\GAL4^sca-C253, aos^UAS.cHa has visible phenotype

(Culi et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, aos^UAS.cHa has wing vein L4 phenotype, enhanceable by Scer\GAL4^en-e16E/msk^UAS.cLa

(Marenda et al., 2006)

Scer\GAL4^en-e16E, aos^UAS.cHa has wing vein L5 phenotype, enhanceable by Scer\GAL4^en-e16E/msk^UAS.cLa

(Marenda et al., 2006)

Scer\GAL4^en-e16E, aos^UAS.cHa has wing vein phenotype, enhanceable by Scer\GAL4^en-e16E/msk^UAS.cLa

(Marenda et al., 2006)

Scer\GAL4^69B, aos^UAS.cHa has wing vein L4 phenotype, enhanceable by Pi3K68D^UAS.Tag:MYC, Scer\GAL4^69B

(MacDougall et al., 2004)

Scer\GAL4^69B, aos^UAS.cHa has anterior crossvein phenotype, enhanceable by Pi3K68D^UAS.Tag:MYC, Scer\GAL4^69B

(MacDougall et al., 2004)

aos^UAS.cHa has wing vein phenotype, enhanceable by Pi3K68D^UAS.Tag:MYC/Scer\GAL4^69B

(MacDougall et al., 2004)

NOT Enhanced by

Statement

Reference

Scer\GAL4^69B, aos^UAS.cHa has wing vein L4 phenotype, non-enhanceable by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, aos^UAS.cHa has eye phenotype, suppressible | partially by rho-5^KO1

(Zettl et al., 2011)

Scer\GAL4^GMR.PF, aos^UAS.cHa has eye phenotype, suppressible | partially by Edem2^RNAi.UAS, Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Scer\GAL4^hs.2sev, aos^UAS.cHa has tertiary pigment cell phenotype, suppressible by lz^ts1/Scer\GAL4^hs.2sev

(Wildonger et al., 2005)

Scer\GAL4^hs.2sev, aos^UAS.cHa has secondary pigment cell phenotype, suppressible by lz^ts1/Scer\GAL4^hs.2sev

(Wildonger et al., 2005)

Scer\GAL4^69B, aos^UAS.cHa has wing vein L4 phenotype, suppressible by Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B

(MacDougall et al., 2004)

Scer\GAL4^69B, aos^UAS.cHa has anterior crossvein phenotype, suppressible by Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B

(MacDougall et al., 2004)

Scer\GAL4^69B, aos^UAS.cHa has wing vein L4 phenotype, suppressible by Df(3L)vin3/+

(MacDougall et al., 2004)

Scer\GAL4^71B, aos^UAS.cHa has wing vein phenotype, suppressible by Scer\GAL4^71B/vn^UAS.cSa

(Wessells et al., 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, aos^UAS.cHa has oenocyte | increased number phenotype, non-suppressible by rho^UAS.cdCa

(Brodu et al., 2004)

Scer\GAL4^69B, aos^UAS.cHa has wing vein L4 phenotype, non-suppressible by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Suppressor of

Statement

Reference

aos^UAS.cHa is a suppressor of embryonic/larval oenocyte precursor | increased number phenotype of Scer\GAL4^en-e16E, rho^UAS.cdCa

(Brodu et al., 2004)

aos^UAS.cHa is a suppressor of oenocyte primordium phenotype of Scer\GAL4^en-e16E, rho^UAS.cdCa

(Brodu et al., 2004)

aos^UAS.cHa is a suppressor of embryonic/larval oenocyte | increased number phenotype of Scer\GAL4^en-e16E, rho^UAS.cdCa

(Brodu et al., 2004)

aos^UAS.cHa/Scer\GAL4^69B is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^69B, vn^ΔIg.UAS

(Donaldson et al., 2004)

aos^UAS.cHa, Scer\GAL4^sca-C253 is a suppressor of mesothoracic tergum & macrochaeta phenotype of Delta^DN.UAS, Scer\GAL4^sca-C253

(Culi et al., 2001)

Scer\GAL4^71B/aos^UAS.cHa is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^71B, vn^UAS.cSa

(Wessells et al., 1999)

Other

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B, aos^UAS.cHa has posterior crossvein phenotype

(MacDougall et al., 2004)

Delta^DN.UAS, Scer\GAL4^sca-C253, aos^UAS.cHa has mesothoracic tergum & macrochaeta phenotype

(Culi et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

A homozygous rho-5^KO1 background suppresses the rough eye phenotype seen when aos^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of sip3^GD3145 suppresses the rough eye phenotype seen when aos^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Edem2^{dsRNA.Scer\UAS} suppresses the rough eye phenotype seen when aos^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

(Zettl et al., 2011)

Coexpression of argos^Scer\UAS.cHa and msk^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E, enhances the vein loss phenotype of argos to 100% in L4 and 100% in L5.

(Marenda et al., 2006)

Expression of argos^Scer\UAS.cHa, under the control of Scer\GAL4^hs.2sev in temperature-shifted lz^ts1 mutants results in a few small clusters of 2^o/3^o cells, which are not seen in argos^Scer\UAS.cHa Scer\GAL4^hs.2sev single mutants.

(Wildonger et al., 2005)

The decreased numbers of oenocytes per cluster seen in argos^Scer\UAS.cHa; Scer\GAL4^en-e16E is not suppressed by rho^{Scer\UAS.cdCa}; Scer\GAL4^en-e16E embryos.

(Brodu et al., 2004)

Co-expression of argos^Scer\UAS.cHa rescues the extra wing vein phenotype caused be expression of vn^{ΔIg.Scer\UAS} under the control of Scer\GAL4^69B.

(Donaldson et al., 2004)

Co-expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} suppresses the loss of wing vein L4 and the anterior crossvein caused by expression of aos^Scer\UAS.cHa under the control of Scer\GAL4^69B, but the posterior crossvein is lost in the double mutant flies.

Co-expression of Pi3K68D^{Scer\UAS.T:Hsap\MYC} enhances the loss of wing veins caused by expression of aos^Scer\UAS.cHa under the control of Scer\GAL4^69B.

Df(3L)vin3/+ suppresses the loss of wing vein L4 seen in flies expressing aos^Scer\UAS.cHa under the control of Scer\GAL4^69B.

Df(3R)Pi3K92E^A/+ has no effect on the loss of wing vein L4 seen in flies expressing aos^Scer\UAS.cHa under the control of Scer\GAL4^69B.

(MacDougall et al., 2004)

Microchaetae and macrochaetae of the notum caused by expression of Dl^D.Scer\UAS driven by Scer\GAL4^sca-C253 are eliminated by co-expression of argos^Scer\UAS.cHa.

(Culi et al., 2001)

The addition of argos^Scer\UAS.cHa produces an phenotype intermediate of those caused by argos^Scer\UAS.cHa or vn^Scer\UAS.cSa alone (driven by Scer\GAL4^71B) the vein loss phenotype of argos^Scer\UAS.cHa is partially rescued, the extra vein phenotype of vn^Scer\UAS.cSa is rescued.

(Wessells et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

aos^Scer\UAS.cHa

aos^UAS.cHa

argos^Scer\UAS.cHa

argos^UAS.cHa

gil^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Howes

(Howes et al., 1998)

Secondary FlyBase IDs

References (24)

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