FlyBase Allele Report: Dmel\sip3[GD3145]

General Information

Symbol

Dmel\sip3^GD3145

Species

D. melanogaster

Name

FlyBase ID

FBal0210589

Feature type

allele

Associated gene

Dmel\sip3

Associated Insertion(s)

Carried in Construct

P{GD3145}

Also Known As

Hrd1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3145}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3145

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Tsou et al., 2015)

exacerbates inclusion body myopathy with Paget disease of bone and frontotemporal dementia

modeled by TER94^A229E.UAS

(Liang et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

(Wang et al., 2014)

midgut | adult stage, with Scer\GAL4^NP0001

(Wang et al., 2014)

midgut | adult stage, with Scer\GAL4^NP5130

(Wang et al., 2014)

midgut | adult stage, with Scer\GAL4^NP5130, Scer\GAL80^Su(H).GBE

(Wang et al., 2014)

midgut | adult stage, with Scer\GAL4^Su(H).GBE

(Wang et al., 2014)

midgut | adult stage | somatic clone, with Scer\GAL4^Act.PU

(Wang et al., 2014)

midgut | adult stage | somatic clone, with Scer\GAL4^unspecified

(Wang et al., 2014)

Detailed Description

Statement

Reference

The expression of sip3^GD3145 under the combined control of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU and Dicer-2 (for efficient RNAi) does not lead to any obvious eye morphology defects, as compared to controls.

(Huang et al., 2018)

The temporally regulated expression (by Gal80[ts]) of sip3^GD3145, for 5 days under the control of Scer\GAL4^NP0001, or for 6 days under the control of Scer\GAL4^NP5130, under the combined control of Scer\GAL4^NP5130 and Scer\GAL80^Su(H).GBE, or under the control of Scer\GAL4^Su(H).GBE, results in a strong increase in the proportion of mitotic cells in adult midguts, as compared to controls; ; expression for 6 days under the control of Scer\GAL4^NP5130 also results in an increase in the proportion of intestinal stem cells, assessed by immmuno-staining for Delta, in adult midguts as compared to controls.clonal expression in the adult midgut also leads to a strong increase in the average clone cell number at 6 days (under the control of Scer\GAL4^Act.PU) or at 4 and 7 days (under the control of Scer\GAL4^unspecified) after clone induction, as compared to control clones.

(Wang et al., 2014)

Adults expressing sip3^GD3145 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3145

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sip3^GD3145, Scer\GAL4^GMR.long is an enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

sip3^GD3145/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, TER94^A229E.UAS

(Liang et al., 2014)

Suppressor of

Statement

Reference

sip3^GD3145/Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA

(Zettl et al., 2011)

sip3^GD3145/Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, aos^UAS.cHa

(Zettl et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

sip3^GD3145, Scer\GAL4^GMR.long is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Tsou et al., 2015)

sip3^GD3145/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, TER94^A229E.UAS

(Liang et al., 2014)

sip3^GD3145/Scer\GAL4^GMR.PF is an enhancer of photoreceptor phenotype of Scer\GAL4^GMR.PF, TER94^A229E.UAS

(Liang et al., 2014)

Suppressor of

Statement

Reference

sip3^GD3145/Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA

(Zettl et al., 2011)

NOT Suppressor of

Statement

Reference

sip3^GD3145, sip3^UAS.Tag:MYC, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

Scer\GAL4^ey.PU, sip3^GD3145/EMC10^KK104001, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

Scer\GAL4^ey.PU, Pngl^KK101641/sip3^GD3145, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

Ube4A^KK101530, sip3^GD3145, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

asrij^KK108914, sip3^GD3145, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

Scer\GAL4^ey.PU, sip3^GD3145/CG2126^GD3969, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

hiro^KK101387, sip3^GD3145, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

Scer\GAL4^ey.PU, CG6766^GD5521/sip3^GD3145, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR

(Huang et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormal eye morphology resulting from the expression of ninaE^G69D.GMR under the combined control of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU is not suppressed by the co-expression of sip3^{Scer\UAS.T:Hsap\MYC} and sip3^GD3145 (together with Dicer-2, for efficient RNAi).

(Huang et al., 2018)

Expression of sip3^GD3145 enhances the rough eye phenotype and underlying photoreceptor degeneration seen when TER94^{A229E.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of sip3^GD3145 does not reverse the suppressive effect of Der-1^Scer\UAS.cLa on the rough eye and photoreceptor degeneration phenotypes seen when TER94^{R188Q.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

(Liang et al., 2014)

Expression of sip3^GD3145 suppresses the rough eye phenotype seen when sty^{Scer\UAS.N.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of sip3^GD3145 suppresses the rough eye phenotype seen when aos^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

(Zettl et al., 2011)

Xenogenetic Interactions

Statement

Reference

Co-expression of sip3^GD3145 enhances eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies. Co-expression of sip3^GD3145 does not suppress the protective role of expression of Hsap\ATXN3^{Scer\UAS.WT.T:Zzzz\His6} in suppression of eye degeneration in Scer\GAL4^GMR.long>Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies.

(Tsou et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v6870

w¹¹¹⁸; P{GD3145}v6870

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3145

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hrd1^RNAi

(Wang et al., 2015)

sip3^GD3145

Name Synonyms

Secondary FlyBase IDs

References (16)

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