FlyBase Allele Report: Dmel\E(spl)mγ-HLH[UAS.cLa]

General Information

Symbol

Dmel\E(spl)mγ-HLH^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ligoxygakis

FlyBase ID

FBal0093290

Feature type

allele

Associated gene

Dmel\E(spl)mγ-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)mγ-HLH.L}

Also Known As

UAS-mγ, UAS-E(spl)mγ

Key Links

Transgenic product class

wild_type

(Ligoxygakis et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ligoxygakis et al., 1998)

Mutagen

in vitro construct

(Ligoxygakis et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-E(spl)mγ-HLH.L}

Cytology

Description

UAS regulatory sequences drive expression of E(spl)mγ-HLH.

(Ligoxygakis et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)mγ-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of central nervous system cancer

CEA

(Zacharioudaki et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GMR9D11

(Janssens et al., 2014)

lethal - all die during embryonic stage, with Scer\GAL4^elav-C155

(Engel and Rand, 2014)

visible, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^455.2

(Apidianakis et al., 1999, Ligoxygakis et al., 1999)

visible, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^sca-537.4

(Nagel et al., 2000)

Phenotype Manifest In

chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4^sca-109-68

(zur Lage and Jarman, 1999)

dorsal row & sensory mother cell, with Scer\GAL4^ap-md544

(Apidianakis et al., 1999)

macrochaeta, with Scer\GAL4^sca-537.4

(Nagel et al., 2000)

scutellar bristle, with Scer\GAL4^455.2

(Apidianakis et al., 1999, Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

type II neuroblast, with Scer\GAL4^GMR9D11

(Janssens et al., 2014)

wing margin bristle, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

wing vein, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

Detailed Description

Statement

Reference

Expression of E(spl)mγ-HLH^Scer\UAS.cLa in the somatic musculature, under the control of Scer\GAL4^Mef2.PU does not affect SNa branching.

Neuronal expression of E(spl)mγ-HLH^Scer\UAS.cLa, under the control of Scer\GAL4^elav-C155 results in lethality in the early embryo.

(Engel and Rand, 2014)

Expression of dpn^Scer\UAS.cWa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) results in supernumerary type II neuroblasts in larval brains. No supernumerary neuroblasts are seen when dpn^Scer\UAS.cWa is expressed in ase[+] immature INPs using P{GMR9D11-GAL4}attP2.

(Janssens et al., 2014)

Expression of HLHmγ^Scer\UAS.cLa under the control of Scer\GAL4^ey.PB has no effect on either eye or antenna disc development.

(Kumar and Moses, 2001)

When HLHmγ^Scer\UAS.cLa is driven by Scer\GAL4^sca-537.4 bristle los is observed on the notum.

(Nagel et al., 2000)

HLHmγ^Scer\UAS.cLa, when driven by Scer\GAL4^ap-md544, causes the loss of the dorsal row of wing margin sensory organ precursors. HLHmγ^Scer\UAS.cLa, when driven by Scer\GAL4^455.2, causes the loss of scutellar bristles.

(Apidianakis et al., 1999)

Flies expressing HLHmγ^Scer\UAS.cLa under the control of Scer\GAL4^32B show severe loss of wing veins. Loss of notum microchaetae and wing margin bristles is seen. Flies expressing HLHmγ^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544 show complete loss of notum microchaetae and macrochaetae, except at the very anterior edge of the notum. Flies expressing HLHmγ^Scer\UAS.cLa under the control of Scer\GAL4^455.2 show severe loss of scutellar bristles. Flies expressing HLHmγ^Scer\UAS.cLa under the control of Scer\GAL4^h-1J3 show loss of notum macrochaetae.

(Ligoxygakis et al., 1999)

Formation of sensory organ precursors (SOPs) of the femoral chordotonal sense organ is suppressed in leg discs in which HLHmγ^Scer\UAS.cLa is expressed under the control of Scer\GAL4^sca-109-68.

(zur Lage and Jarman, 1999)

Flies expressing one copy of HLHmγ^Scer\UAS.cLa under the control of one copy of Scer\GAL4^h-H10 are phenotypically wild-type.

(Ligoxygakis et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11 has abnormal neuroanatomy phenotype, suppressible by erm^UAS.Tag:HA, Scer\GAL4^GMR9D11

(Janssens et al., 2014)

NOT suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^ptc-559.1 has visible phenotype, non-suppressible by E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11 is an enhancer of abnormal neuroanatomy phenotype of Df(2L)erm²/erm¹, erm^Tag:FLAG

(Janssens et al., 2014)

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^32B is an enhancer of visible | recessive | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11 is a suppressor of abnormal neuroanatomy phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^32B is a suppressor of visible | recessive | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Other

Statement

Reference

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11, erm^zf.VP16.UAS has abnormal neuroanatomy phenotype

(Janssens et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11 has type II neuroblast phenotype, suppressible by erm^UAS.Tag:HA, Scer\GAL4^GMR9D11

(Janssens et al., 2014)

NOT suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^ptc-559.1 has scutellar bristle phenotype, non-suppressible by E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11 is an enhancer of type II neuroblast | increased number phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^GMR9D11 is an enhancer of type II neuroblast | increased number phenotype of Df(2L)erm²/erm¹, erm^Tag:FLAG

(Janssens et al., 2014)

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^32B is an enhancer of wing | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^pnr-MD237 is a suppressor of chaeta | ectopic phenotype of Scer\GAL4^pnr-MD237, sc^UAS.cPa

(Giagtzoglou et al., 2003)

E(spl)mγ-HLH^UAS.cLa/Scer\GAL4^32B is a suppressor of wing | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Other

Statement

Reference

E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11, erm^zf.VP16.UAS has type II neuroblast | increased number phenotype

(Janssens et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of E(spl)mγ-HLH^Scer\UAS.cLa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) strongly enhances the supernumerary neuroblast phenotype seen in brat^DG19310/brat¹¹ mutant larval brains. A milder enhancement is seen when E(spl)mγ-HLH^Scer\UAS.cLa is expressed using an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2).

Expression of E(spl)mγ-HLH^Scer\UAS.cLa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of erm^{Scer\UAS.T:Ivir\HA1} suppresses the supernumerary type II neuroblasts phenotype seen in larval brains when E(spl)mγ-HLH^Scer\UAS.cLa is expressed under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2).

In contrast to when either transgene is expressed alone, co-expression of erm^{zf.Scer\UAS.T:Hsim\VP16} and E(spl)mγ-HLH^Scer\UAS.cLa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) results in supernumerary type II neuroblasts in the larval brain.

(Janssens et al., 2014)

Co-expression of HLHmγ^Scer\UAS.cLa and sc^Scer\UAS.cPa under the control of Scer\GAL4^pnr-MD237 results in adult flies with a bald stripe in the centre of the thorax (the formation of ectopic chaetae caused by expression of sc^Scer\UAS.cPa under the control of Scer\GAL4^pnr-MD237 is suppressed in these flies).

(Giagtzoglou et al., 2003)

HLHmγ^Scer\UAS.cLa expressed under the control of Scer\GAL4^32B restores the distal wing margin and adjacent wing blade tissue that is missing in Df(1)N-54l9 heterozygotes. Expression of HLHmγ^Scer\UAS.cLa under the control of Scer\GAL4^32B enhances the scalloped wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the early third larval instar stage (72-84 hours after egg laying). Expression of HLHmγ^Scer\UAS.cLa under the control of Scer\GAL4^32B suppresses the nicked wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the mid third larval instar stage (96-108 hours after egg laying).

(Ligoxygakis et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The relative ability of UAS constructs of the E(spl) complex to induce wing vein deletion when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHmβ^{Scer\UAS.cdCa} = HLHmγ^Scer\UAS.cLa > E(spl)^Scer\UAS.cNa = HLHm7^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum microchaetae when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} >> HLHmγ^Scer\UAS.cLa = HLHmβ^{Scer\UAS.cdCa} = HLHm3^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of wing margin bristles when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHm7^{Scer\UAS.cdCa} > E(spl)^Scer\UAS.cNa > HLHmγ^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum macrochaetae when expressed under the control of Scer\GAL4^h-1J3 is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa} > HLHmγ^Scer\UAS.cLa = HLHm3^Scer\UAS.cLa.

(Ligoxygakis et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(spl)mγ-HLH^Scer\UAS.cLa

E(spl)mγ-HLH^UAS.cLa

HLHmγ^Scer\UAS.cLa

HLHmγ^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ligoxygakis

(Ligoxygakis et al., 1998)

Secondary FlyBase IDs

References (12)

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