FlyBase Allele Report: Dmel\E(spl)mα-BFM[UAS.cAa]

General Information

Symbol

Dmel\E(spl)mα-BFM^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Apidianakis

FlyBase ID

FBal0101270

Feature type

allele

Associated gene

Dmel\E(spl)mα-BFM

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)mα-BFM.A}

Also Known As

UAS-mα

Key Links

Transgenic product class

cDNA

(Apidianakis et al., 1999)

wild_type

(Apidianakis et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Apidianakis et al., 1999)

wild_type

(Apidianakis et al., 1999)

Mutagen

in vitro construct

(Apidianakis et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-E(spl)mα-BFM.A}

Cytology

Description

The complete E(spl)mα-BFM cDNA is under the control of UASt sequences.

(Apidianakis et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

E(spl)mα-BFM

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^455.2

(Apidianakis et al., 1999)

visible, with Scer\GAL4^ap-md544

(Apidianakis et al., 1999)

visible, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

visible, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

visible, with Scer\GAL4^sca-T3

(Apidianakis et al., 1999)

Phenotype Manifest In

campaniform sensillum, with Scer\GAL4^455.2

(Apidianakis et al., 1999)

campaniform sensillum, with Scer\GAL4^ap-md544

(Apidianakis et al., 1999)

campaniform sensillum, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

campaniform sensillum, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

campaniform sensillum, with Scer\GAL4^sca-T3

(Apidianakis et al., 1999)

dorsal row & sensory mother cell, with Scer\GAL4^ap-md544

(Apidianakis et al., 1999)

dorsocentral bristle, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

eo neuron, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

macrochaeta, with Scer\GAL4^455.2

(Apidianakis et al., 1999)

macrochaeta, with Scer\GAL4^ap-md544

(Apidianakis et al., 1999)

macrochaeta, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

macrochaeta, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

macrochaeta, with Scer\GAL4^sca-T3

(Apidianakis et al., 1999)

macrochaeta & scutum, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

microchaeta, with Scer\GAL4^455.2

(Apidianakis et al., 1999)

microchaeta, with Scer\GAL4^ap-md544

(Apidianakis et al., 1999)

microchaeta, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

microchaeta, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

microchaeta, with Scer\GAL4^sca-T3

(Apidianakis et al., 1999)

microchaeta & scutum, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

scutellar bristle, with Scer\GAL4^455.2

(Apidianakis et al., 1999)

sensory mother cell | ectopic, with Scer\GAL4^bi-omb-Gal4

(Nagel et al., 2000)

thecogen cell, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

tormogen cell, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

trichogen cell, with Scer\GAL4^pnr-MD237

(Apidianakis et al., 1999)

Detailed Description

Statement

Reference

Expression of mα^Scer\UAS.cAa under the control of Scer\GAL4^ey.PB has no effect on either eye or antenna disc development.

(Kumar and Moses, 2001)

Expression of mα^Scer\UAS.cAa when driven by Scer\GAL4^bi-omb-Gal4 in the wing disc leads to an increase in the numbers of sensory organ precursors seen.

(Nagel et al., 2000)

Flies with mα^Scer\UAS.cAa under the control of Scer\GAL4^ptc-559.1, Scer\GAL4^sca-T3, Scer\GAL4^455.2, Scer\GAL4^pnr-MD237 or Scer\GAL4^ap-md544, show duplications of sensillum campaniformium, macrochaetae and microchaetae in their normal locations that are frequently tufted together. No effects are seen in the wing. Flies with mα^Scer\UAS.cAa under the control of Scer\GAL4^pnr-MD237, show multiplication of all chaetae between the two dorsocentral rows. Flies with mα^Scer\UAS.cAa under the control of Scer\GAL4^32B or Scer\GAL4^bi-md653 showed no wing pattern aberrations. mα^Scer\UAS.cAa, when driven by Scer\GAL4^ap-md544, creates clusters of wing margin sensory organ precursors (SOPs) in their normal locations in the dorsal row, where there would only be single SOPs in wild-type. The N/numb mediated asymmetric divisions in the SOP lineage are not affected. When mα^Scer\UAS.cAa is driven by Scer\GAL4^pnr-MD237 the duplications of the SOPs is accompanied by the equivalent duplication of their descendents, i.e the trichogen cell and tormogen cell, and the thecogen cell and its associated eo neuron, equal numbers of each being seen.

(Apidianakis et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has visible phenotype, enhanceable by N^264-40

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has visible phenotype, enhanceable by Df(3R)gro^b32.2

(Apidianakis et al., 1999)

NOT Enhanced by

Statement

Reference

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has visible phenotype, non-enhanceable by N^Ax-1

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has visible phenotype, non-enhanceable by Dp(1;2)N⁺

(Apidianakis et al., 1999)

NOT suppressed by

Statement

Reference

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has visible phenotype, non-suppressible by N^Ax-1

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has visible phenotype, non-suppressible by Dp(1;2)N⁺

(Apidianakis et al., 1999)

NOT Suppressor of

Statement

Reference

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of E(spl)mδ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of E(spl)m8-HLH^UAS.cLa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has scutellar bristle phenotype, enhanceable by N^264-40

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^455.2 has scutellar bristle phenotype, enhanceable by Df(3R)gro^b32.2

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1 is an enhancer of scutellar bristle | ectopic phenotype of E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, E(spl)mα-BFM^UAS.cAa is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of N^264-39

(Lu and Li, 2015)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of scutellar bristle phenotype of E(spl)mδ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of scutellar bristle phenotype of E(spl)m8-HLH^UAS.cLa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)mα-BFM^UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of scutellar bristle phenotype of E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

mα^Scer\UAS.cAa driven by Scer\GAL4^455.2 cannot restore scutellar bristles to E(spl)^Scer\UAS.cLa, HLHmδ^{Scer\UAS.cdCa} or HLHmγ^Scer\UAS.cLa. The presence of N^264-40 enhances the supernumerary scutellar bristle phenotype seen in flies with mα^Scer\UAS.cAa driven by Scer\GAL4^455.2. The presence of N^Ax-1 or Dp(1;2)N⁺ reduces the number of supernumerary scutellar bristles seen in flies with mα^Scer\UAS.cAa driven by Scer\GAL4^455.2, though the number seen is still significantly higher than wild-type. The presence of Df(3R)gro^b32.2 enhances the supernumerary scutellar bristle phenotype seen in flies with mα^Scer\UAS.cAa driven by Scer\GAL4^455.2.

(Apidianakis et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27179

y¹ w^*; P{UAS-E(spl)mα-BFM.A}A17.4

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

E(spl)mα-BFM^Scer\UAS.cAa

E(spl)mα-BFM^UAS.cAa

mα^Scer\UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Apidianakis

(Apidianakis et al., 1999)

Secondary FlyBase IDs

References (7)

Report Sections

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