FlyBase Allele Report: Dmel\spen[poc361]

General Information

Symbol

Dmel\spen^poc361

Species

D. melanogaster

Name

FlyBase ID

FBal0101141

Feature type

allele

Associated gene

Dmel\spen

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | somatic clone

(Mace and Tugores, 2004)

lethal | recessive

(Wiellette et al., 1999)

visible | somatic clone

(Mace and Tugores, 2004)

Phenotype Manifest In

abdominal lateral pentascolopidial chordotonal organ lch5 | germline clone

(Kuang et al., 2000)

crossvein | somatic clone

(Mace and Tugores, 2004)

dental sclerite

(Wiellette et al., 1999)

dorsal bridge

(Wiellette et al., 1999)

embryonic/larval somatic muscle cell | germline clone

(Kuang et al., 2000)

embryonic epidermis

(Mace et al., 2005)

embryonic head

(Wiellette et al., 1999)

embryonic mesothoracic segment & sclerite | ectopic

(Wiellette et al., 1999)

embryonic metathoracic segment & sclerite | ectopic

(Wiellette et al., 1999)

epipharyngeal sclerite

(Wiellette et al., 1999)

hypostomal sclerite

(Wiellette et al., 1999)

larval longitudinal connective | germline clone

(Kuang et al., 2000)

larval ventral nerve cord commissure | germline clone

(Kuang et al., 2000)

lateral process of cephalopharyngeal skeleton

(Wiellette et al., 1999)

macrochaeta | somatic clone

(Mace and Tugores, 2004)

median tooth

(Wiellette et al., 1999)

microchaeta | somatic clone

(Mace and Tugores, 2004)

motor neuron | embryonic stage | germline clone

(Kuang et al., 2000)

mouth hook

(Wiellette et al., 1999)

presumptive embryonic/larval central nervous system | germline clone

(Kuang et al., 2000)

presumptive embryonic/larval peripheral nervous system

(Mace and Tugores, 2004)

presumptive embryonic/larval peripheral nervous system | germline clone

(Kuang et al., 2000)

sclerite & embryonic abdominal segment | ectopic

(Wiellette et al., 1999)

ventral midline of embryo | germline clone

(Kuang et al., 2000)

wing hair | somatic clone

(Mace and Tugores, 2004)

wing margin bristle | somatic clone

(Mace and Tugores, 2004)

wing vein | ectopic | somatic clone

(Mace and Tugores, 2004)

wing vein | somatic clone

(Mace and Tugores, 2004)

Detailed Description

Statement

Reference

Mutant embryos develop ectopic sclerites, which often look like the cuticular scar tissue that often surrounds the healed wound generated by a sterile needle in late-stage wild-type embryos.

Mutant stage 17 embryos have failures in epidermal integrity; dye injected into the perivitelline space penetrates the body cavity in the mutant embryos.

(Mace et al., 2005)

Generation of spen^poc361 mitotic clones in the wing imaginal disc, using the FLP/FRT technique, causes a wing vein phenotype in the adult. This includes loss of vein material, which is mostly in the distal part of the wing, ectopic formation of material around the veins, thickening of existing veins and slight mis-localization of both longitudinal and cross-veins. Wing hair polarity is also disrupted and bristles are misplaced at the wing margin. Generation of spen^poc361 mitotic clones in the anterior compartment of the wing disc, using the FLP/FRT technique, affects both the micro and macrochaetae on the notum of adults. Macrochaetae are lost in some parts of the notum, gained in others and mislocalized. Duplicated trichogens and thormogens are not observed, indicating that cell fate specification is not affected. In both spen^poc361 maternal and zygotic embryos, the neurons of the peripheral nervous system show an abnormal distribution and morphology, and can be either fewer or greater in number than in wild type.

(Mace and Tugores, 2004)

Mutant embryos derived from females containing homozygous spen^poc361 germline clones crossed to spen³/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. The number of lateral chordotonal organs in each abdominal hemisegment varies from 0 to 6, and is typically 4 (wild-type number is 5). Clusters containing the normal number are often disorganised. Midline development is defective. Commissures are missing or poorly separated in stage 15 embryos. All of the longitudinal axons are disrupted in stage 16 embryos and axons inappropriately cross the midline. The development of all motor axons pathways is defective; motor axons exit the central nervous system, pick the correct pathways, but fail to innervate their muscle targets. Muscle fibres are missing or disorganised.

(Kuang et al., 2000)

Hemizygotes show loss of anterior H piece and ectopic sclerites develop in the middle of T2 and T3. These ectopic sclerites do not form in the fields of the denticle belts. When the maternal contribution is reduced the phenotype is more marked, with the posterior mouth hooks, median tooth, hypostomal, ectostomal, and epistomal sclerites, dorsal bridge and lateralgraten all either missing or disrupted. The trunk region of the embryos appears to be largely unaffected. Denticle belts and filzkorper show slightly reduced pigmentation.

(Wiellette et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

spen^poc361 has visible | somatic clone phenotype, enhanceable by ck^unspecified

(Mace and Tugores, 2004)

Enhancer of

Statement

Reference

spen^poc361 is an enhancer of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Suppressor of

Statement

Reference

spen[+]/spen^poc361 is a suppressor of visible phenotype of CycE^JP

(Brumby et al., 2004)

spen^poc361 is a suppressor of visible phenotype of CycE^AR95, dap^GMR.PdN

(Staehling-Hampton et al., 1999)

spen^poc361 is a suppressor of visible phenotype of Hsap\CDKN1A^GMR.PH

(Staehling-Hampton et al., 1999)

spen^poc361 is a suppressor of decreased occurrence of cell division | larval stage phenotype of Hsap\CDKN1A^GMR.PH

(Staehling-Hampton et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

spen^poc361 has crossvein | somatic clone phenotype, enhanceable by ck^unspecified

(Mace and Tugores, 2004)

spen^poc361 has wing hair | somatic clone phenotype, enhanceable by ck^unspecified

(Mace and Tugores, 2004)

spen^poc361 has wing margin bristle | somatic clone phenotype, enhanceable by ck^unspecified

(Mace and Tugores, 2004)

spen^poc361 has wing vein | ectopic | somatic clone phenotype, enhanceable by ck^unspecified

(Mace and Tugores, 2004)

spen^poc361 has wing vein | somatic clone phenotype, enhanceable by ck^unspecified

(Mace and Tugores, 2004)

Suppressed by

Statement

Reference

spen^poc361 has phenotype, suppressible by Antp^hs.PHTA

(Wiellette et al., 1999)

spen^poc361 has embryonic mesothoracic segment & sclerite | ectopic phenotype, suppressible by tsh^UAS.cGa/Scer\GAL4^69B

(Wiellette et al., 1999)

spen^poc361 has embryonic metathoracic segment & sclerite | ectopic phenotype, suppressible by tsh^UAS.cGa/Scer\GAL4^69B

(Wiellette et al., 1999)

Enhancer of

Statement

Reference

spen^poc361 is an enhancer of eye phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Suppressor of

Statement

Reference

spen[+]/spen^poc361 is a suppressor of eye phenotype of CycE^JP

(Brumby et al., 2004)

spen^poc361 is a suppressor of eye phenotype of CycE^AR95, dap^GMR.PdN

(Staehling-Hampton et al., 1999)

spen^poc361 is a suppressor of ommatidium phenotype of CycE^AR95, dap^GMR.PdN

(Staehling-Hampton et al., 1999)

spen^poc361 is a suppressor of eye phenotype of Hsap\CDKN1A^GMR.PH

(Staehling-Hampton et al., 1999)

spen^poc361 is a suppressor of ommatidium phenotype of Hsap\CDKN1A^GMR.PH

(Staehling-Hampton et al., 1999)

spen^poc361 is a suppressor of eye disc phenotype of Hsap\CDKN1A^GMR.PH

(Staehling-Hampton et al., 1999)

Other

Statement

Reference

Dp^GMR.PD, E2f1^GMR.PD, spen^poc361 has interommatidial bristle phenotype

(Staehling-Hampton et al., 1999)

Dp^GMR.PD, E2f1^GMR.PD, spen^poc361 has eye disc phenotype

(Staehling-Hampton et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The additional presence of a ck^unspecified mutation in mitotic spen^poc361 clones, generated in the wing disc, enhances the wing phenotype in adults. There is a greater excess of vein material in the double mutants, more misplacement of longitudinal and cross veins, and more misalignment and tufting of the thick trichomes at the wing margin. These clones are non-autonomous as they can affect vein morphology on the dorsal side of the wing when the clones are ventral and vice versa.

(Mace and Tugores, 2004)

Enhances the rough eye phenotype of E2f^GMR.PD Dp^GMR.PD flies; the eyes are rougher and have more bristles. The second wave of S phases is abnormal in eye discs of these animals.

(Staehling-Hampton et al., 1999)

Xenogenetic Interactions

Statement

Reference

Flies expressing Hsap\CDKN1A^GMR.PH have rough eyes. This phenotype is suppressed by spen^poc361. The second mitotic wave is absent in eye imaginal discs expressing Hsap\CDKN1A^GMR.PH. This wave is partially restored in the presence of one copy of spen^poc361.

(Staehling-Hampton et al., 1999)

Complementation and Rescue Data

Fails to complement

spen^ksh-1

(Staehling-Hampton et al., 1999)

spen^ksh-2

(Staehling-Hampton et al., 1999)

spen^ksh-3

(Staehling-Hampton et al., 1999)

spen^ksh-4

(Staehling-Hampton et al., 1999)

spen^pjc-15