FlyBase Allele Report: Dmel\Tak1[UAS.cTa]

General Information

Symbol

Dmel\Tak1^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Takatsu

FlyBase ID

FBal0117635

Feature type

allele

Associated gene

Dmel\Tak1

Associated Insertion(s)

Carried in Construct

P{UAS-Tak1}

Also Known As

UAS-DTak1, tak1, UAS-dTak

Key Links

Transgenic product class

cDNA

(Takatsu et al., 2000)

wild_type

(Takatsu et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Takatsu et al., 2000)

wild_type

(Takatsu et al., 2000)

Mutagen

in vitro construct

(Takatsu et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Tak1}

Cytology

Description

The full length Tak1 cDNA is expressed under the control of UASt sequences.

(Takatsu et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tak1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle row 1, with Scer\GAL4^prd.RG1

(McEwen et al., 2000)

eye, with Scer\GAL4^GMR.PF

(Shin et al., 2013, Mallik and Lakhotia, 2009, Tiwari and Roy, 2009, Takatsu et al., 2000)

eye disc, with Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

neuroblast | larval stage, with Scer\GAL4^wor.PA

(Kuang et al., 2014)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

pupa, with Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

ventral denticle belt, with Scer\GAL4^prd.RG1

(McEwen et al., 2000)

Detailed Description

Statement

Reference

Expression of Tak1^Scer\UAS.cTa under the control of Scer\GAL4^wor.PA induces neuroblast loss in larval brains.

(Kuang et al., 2014)

Overexpression of Tak1^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF, induces autophagy in developing eye discs. This results in a lack of eye pigmentation and a rough, impaired appearance compared with control flies.

(Shin et al., 2013)

Scer\GAL4^GMR.PF-driven expression of Tak1^Scer\UAS.cTa results in a non-pigmented and small eye and lethality of 45% of differentiated pupae.

(Mallik and Lakhotia, 2009)

Expression of Tak1^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF results in a small eye phenotype.

(Tiwari and Roy, 2009)

When expression is driven by Scer\GAL4^prd.RG1, anterior rows of denticles are lost in even numbered segments.

(McEwen et al., 2000)

most Tak1^Scer\UAS.cTa,Scer\GAL4^GMR.PF animals die at pupal stages when grown at 25^oC. They exhibit a small eye phenotype. The number of apoptotic cells increases dramatically in regions posterior to the morphogenetic furrow in the developing eye. Sequential recruitment of the photoreceptor cells is disrupted in mutant eye discs. Early photoreceptor induction appears to occur normally but later photoreceptor induction is delayed or disrupted and photoreceptor clustering shows an abnormal and diffuse pattern.

(Takatsu et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has increased cell death phenotype, enhanceable by S6k^KQ.UAS, Scer\GAL4^GMR.PF

(Shin et al., 2013)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has lethal | pupal stage phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has lethal | pupal stage phenotype, non-enhanceable by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act.PU, Tak1^UAS.cTa has neoplasia | somatic clone | cell autonomous | third instar larval stage phenotype, suppressible | cell non-autonomous by Tak1¹

(Chabu and Xu, 2014)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has increased cell death phenotype, suppressible by S6k^UAS.cMa, Scer\GAL4^GMR.PF

(Shin et al., 2013)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Shin et al., 2013)

Scer\GAL4^how-24B, Tak1^UAS.cTa has increased cell death | embryonic stage phenotype, suppressible by Scer\GAL4^how-24B/bsk^DN.UAS.cUa

(Yang and Su, 2011)

Scer\GAL4^how-24B, Tak1^UAS.cTa has increased cell death | embryonic stage phenotype, suppressible by p53^DN.UAS.cUa/Scer\GAL4^how-24B

(Yang and Su, 2011)

Scer\GAL4^how-24B, Tak1^UAS.cTa has increased cell death | embryonic stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^how-24B

(Yang and Su, 2011)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has lethal | pupal stage phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Tak1^UAS.cTa has visible phenotype, suppressible by BacA\p35^GMR.PH/Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has visible phenotype, suppressible by Df(3L)H99

(Takatsu et al., 2000)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has visible phenotype, suppressible by bsk²

(Takatsu et al., 2000)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has visible phenotype, suppressible by hep¹

(Takatsu et al., 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^wor.PA, Tak1^UAS.cTa has abnormal neuroanatomy | larval stage phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^wor.PA

(Kuang et al., 2014)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has visible phenotype, non-suppressible by Dsor1^LH110

(Takatsu et al., 2000)

Other

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^GMR.PF, Tak1^UAS.cTa has lethal - all die before end of pupal stage phenotype

(Shin et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, enhanceable by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, enhanceable by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act.PU, Tak1^UAS.cTa has eye disc | somatic clone | cell autonomous | third instar larval stage phenotype, suppressible | cell non-autonomous by Tak1¹

(Chabu and Xu, 2014)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, suppressible by hep¹

(Takatsu et al., 2000)

Tak1^UAS.cTa has eye phenotype, suppressible by BacA\p35^GMR.PH/Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, suppressible by Df(3L)H99

(Takatsu et al., 2000)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, suppressible by bsk²

(Takatsu et al., 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^wor.PA, Tak1^UAS.cTa has neuroblast | larval stage phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^wor.PA

(Kuang et al., 2014)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Shin et al., 2013)

Scer\GAL4^GMR.PF, Tak1^UAS.cTa has eye phenotype, non-suppressible by Dsor1^LH110

(Takatsu et al., 2000)

Enhancer of

Statement

Reference

Tak1^UAS.cTa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Rab11^N124I.UAS, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Tak1^UAS.cTa/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Tak1^UAS.cTa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Tak1^UAS.cTa/Scer\GAL4^GMR.PF is an enhancer of eye photoreceptor cell phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^LE/Tak1^UAS.cTa is a suppressor of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

Scer\GAL4^prd.RG1/Tak1^UAS.cTa is a suppressor of embryonic epidermis | ventral phenotype of arm³

(McEwen et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The number of autolysosomes, indicating the level of autophagy, is increased upon co-expression of S6k^KQ.Scer\UAS and Tak1^Scer\UAS.cTa in the developing eye disc, under the control of Scer\GAL4^GMR.PF, compared to expression of either transgene alone.

The number of autolysosomes, indicating the level of autophagy, is decreased upon co-expression of S6k^Scer\UAS.cMa and Tak1^Scer\UAS.cTa in the developing eye disc, under the control of Scer\GAL4^GMR.PF, compared to expression of Tak1^Scer\UAS.cTa alone.

(Shin et al., 2013)

Co-expression of P{Sym-UAS-Hsrω} suppresses the eye phenotype of Scer\GAL4^GMR.PF, Tak1^Scer\UAS.cTa, and also substantially reduces the mortality of differentiated pupae.

Coexpression of Hsrω^EP93D or Hsrω^EP3037 enhances the Scer\GAL4^GMR.PF, Tak1^Scer\UAS.cTa eye phenotype, though pupal lethality is not increased.

(Mallik and Lakhotia, 2009)

The reduced eye phenotype caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Tak1^Scer\UAS.cTa.

(Tiwari and Roy, 2009)

Coexpression of Tak1^Scer\UAS.cTa, with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, an increased eye roughness and reduced eye size compared to expression of just Traf1^EP578.

(Cha et al., 2003)

The addition of BacA\p35^GMR.PH to Tak1^Scer\UAS.cTa,Scer\GAL4^GMR.PF flies suppresses the cell death phenotype in the eye, but has no effect on the abnormal photoreceptor clustering seen in the eye disc. The addition of hep¹/Y to Tak1^Scer\UAS.cTa,Scer\GAL4^GMR.PF flies the reduced eye size phenotype is rescued almost to wild-type. The addition of bsk²/+ to Tak1^Scer\UAS.cTa,Scer\GAL4^GMR.PF flies the reduced eye size phenotype is rescued almost to wild-type.

(Takatsu et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa does not suppress the neuroblast loss seen in larval brains expressing Tak1^Scer\UAS.cTa under the control of Scer\GAL4^wor.PA.

(Kuang et al., 2014)

Despite suppression of apoptosis through expression of BacA\p35^Scer\UAS.cHa, a rough, impaired adult eye phenotype is found in BacA\p35^Scer\UAS.cHa/Scer\GAL4^GMR.PF; Tak1^Scer\UAS.cTa flies, indicating that the Tak1-induced impaired eye phenotype is mainly due to autophagy.

(Shin et al., 2013)

Complementation and Rescue Data

Partially rescues

Tak1^UAS.cTa/Scer\GAL4^hs.PB partially rescues Tak1¹

(Vidal et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tak1^Scer\UAS.cTa

Tak1^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Takatsu

(Takatsu et al., 2000)

Secondary FlyBase IDs

References (15)

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