FlyBase Allele Report: Dmel\tim[UAS.cYa]

General Information

Symbol

Dmel\tim^UAS.cYa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Yang

FlyBase ID

FBal0121508

Feature type

allele

Associated gene

Dmel\tim

Associated Insertion(s)

Carried in Construct

P{UAS-tim.Y}

Also Known As

UAS-tim

Key Links

Transgenic product class

wild_type

(Yang and Sehgal, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yang and Sehgal, 2001)

Mutagen

in vitro construct

(Yang and Sehgal, 2001)

Progenitor genotype

Carried in construct

P{UAS-tim.Y}

Cytology

Description

Expression of tim is governed by UAS regulatory sequences.

(Yang and Sehgal, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tim

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult heart, with Scer\GAL4^Hand.PU

(Tang et al., 2024)

Detailed Description

Statement

Reference

Overexpression of tim^Scer\UAS.cYa in PDF neurons under the control of Scer\GAL4^P2.4.Pdf does not eliminate circadian rhythms completely, but leads to an increased incidence of arrhythmia. However, no change in fat body triglycerides is observed.

(Diangelo et al., 2011)

Flies expressing tim^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE in a Scer\GAL80^Pdf.PS background show rhythmic locomotor activity under constant light conditions, in contrast to wild-type flies, which are uniformly arrhythmic under these conditions.

(Stoleru et al., 2007)

When tim^Scer\UAS.cYa is driven by Scer\GAL4^Mai-60, eclosion rhythms are disrupted. Although peaks are present in the eclosion profile, a rhythm and gating are absent. Adult locomotor activity remains rhythmic as in wild-type. When tim^Scer\UAS.cYa is driven by Scer\GAL4^elav-C155 the molecular clock in the lateral neurons is disrupted, and eclosion remains arrhythmic.

(Myers et al., 2003)

When expression is driven in a tim⁺ background by Scer\GAL4^elav-C155, Scer\GAL4^Act5C.PI or Scer\GAL4^tim.PE arrythmicity occurs. Two copies of P{UAS-tim.Y} give a stronger effect than one. The effect is not evident with Scer\GAL4^P2.4.Pdf.

(Yang and Sehgal, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

tim^UAS.cYa/Scer\GAL4^Pdf.Switch1a is a suppressor | partially of abnormal locomotor rhythm | adult stage | RU486 conditional phenotype of Scer\GAL4^Pdf.Switch1a, mir-276a^UAS.pLUC

(Chen and Rosbash, 2016)

tim^UAS.cYa/Scer\GAL4^P2.4.Pdf is a suppressor of abnormal circadian rhythm phenotype of Scer\GAL4^P2.4.Pdf, per^UAS.cYa

(Beckwith et al., 2013)

NOT Suppressor of

Statement

Reference

tim^UAS.cYa/Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal locomotor rhythm phenotype of tyf^ΔG14151

(Lim et al., 2011)

tim^UAS.cYa/Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal locomotor rhythm phenotype of tyf^e00614

(Lim et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The severe behavioral arrhythmicity (~70% of flies display arrhythmic circadian activity) observed in flies upon adult-onset expression of mir-276a^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^Pdf.Switch1a (used with RU486-supplemented diet to control the time of expression) is partially rescued by co-expression of tim^Scer\UAS.cYa.

(Chen and Rosbash, 2016)

Co-expression of per^Scer\UAS.cYa and tim^Scer\UAS.cYa under the control of Scer\GAL4^P2.4.Pdf results in highly rhythmic flies, with periods close to 24 hours.

(Beckwith et al., 2013)

The weakened rhythmicity seen in tyf^ΔG14151 and tyf^e00614 flies under constant darkness conditions is not suppressed by expression of tim^Scer\UAS.cYa under the control of Scer\GAL4^P2.4.Pdf.

(Lim et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

tim^UAS.cYa/Scer\GAL4^elav-C155 partially rescues tim⁰¹

(Myers et al., 2003)

tim^UAS.cYa/Scer\GAL4^elav-C155 partially rescues tim⁰¹

(Yang and Sehgal, 2001)

Comments

tim^Scer\UAS.cYa driven by Scer\GAL4^elav-C155 does not rescue eclosion rhythms, as it does locomotor rhythms in adults.

(Myers et al., 2003)

The degree of rescue of the tim⁰¹ locomotor rhythm defects depends to some extent on the site of insertion of the rescuing P{UAS-tim.Y} transgene and is maximally ~70% and minimally 3.1%. One line, P{UAS-tim.Y}3-1 fails to rescue.

(Yang and Sehgal, 2001)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80686

w^*; P{UAS-tim.Y}2-1/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tim^Scer\UAS.cYa

tim^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Yang

(Yang and Sehgal, 2001)

Secondary FlyBase IDs

References (10)

Report Sections

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