FB2024_03 , released June 25, 2024
Allele: Dmel\mir-276aUAS.pLUC
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General Information
Symbol
Dmel\mir-276aUAS.pLUC
Species
D. melanogaster
Name
FlyBase ID
FBal0277366
Feature type
allele
Associated gene
Dmel\mir-276a
Associated Insertion(s)
Carried in Construct
P{UAS-LUC-mir-276a.T}
Also Known As
UAS-mir-276a
Key Links
Transgenic product class
wild_type
(Bejarano et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bejarano et al., 2012)
Mutagen
in vitro construct
(Bejarano et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-LUC-mir-276a.T}
Cytology
Description

UAS regulatory sequences drive expression of Ppyr\LUC followed by a genomic fragment containing the mir-276a pre-miRNA hairpin.

(Bejarano et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mir-276a
Tagged with
pLUC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      individuals expressing mir-276aUAS.pLUC under the control of Scer\GAL4lncRNA:CR46006-0168-G4 show decreased day-time sleep, with decreased sleep episode duration.

      individuals expressing mir-276aUAS.pLUC under the control Scer\GAL4tim.PE show decreased day-time and night-time sleep.

      individuals expressing mir-276aUAS.pLUC under the control of Scer\GAL4Ilp2.PR or Scer\GAL4Mef2.247 show decreased day-time sleep.

      (Zhang et al., 2021)

      Expression of mir-276aScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4P2.4.Pdf or Scer\GAL4tim.PE results in arrhythmic circadian activity in 50% and 82% of adult flies, respectively. Expression limited to adulthood (by using the Scer\GAL4Pdf.Switch1a driver and RU486-supplemented diet to activate the expression in adult flies only) also leads to severe behavioral arrhythmicity, with ~70% of flies arrhythmic. Circadian free-running period in the Scer\GAL4tim.PE>mir-276aScer\UAS.T:Ppyr\LUC flies that remain (at least partially) rhythmic is lower than in controls, but most likely just due to inaccuracy of the measurements caused by the decreased rhythmicity as with the other drivers the difference is either insignificant or very weak.

      (Chen and Rosbash, 2016)

      Expression of mir-276aScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4sd-SG29.1 results in a weak wing notching phenotype.

      (Bejarano et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The severe behavioral arrhythmicity (~70% of flies display arrhythmic circadian activity) observed in flies upon adult-onset expression of mir-276aScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4Pdf.Switch1a (used with RU486-supplemented diet to control the time of expression) is partially rescued by co-expression of timScer\UAS.cYa.

      (Chen and Rosbash, 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mir-276aScer\UAS.T:Ppyr\LUC
      mir-276aUAS.pLUC
      Name Synonyms
      Secondary FlyBase IDs
        References (5)