FB2024_03 , released June 25, 2024
Allele: Dmel\AktUAS.Tag:HA,Tag:MyrPalm(Lck)
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General Information
Symbol
Dmel\AktUAS.Tag:HA,Tag:MyrPalm(Lck)
Species
D. melanogaster
Name
FlyBase ID
FBal0137673
Feature type
allele
Associated gene
Dmel\Akt
Associated Insertion(s)
Carried in Construct
P{UAS-myr-Akt.S}
Also Known As
UAS-myr-Akt, UAS-myrAkt
Key Links
Transgenic product class
wild_type
(Stocker et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Stocker et al., 2002)
Mutagen
in vitro construct
(Stocker et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-myr-Akt.S}
Cytology
Description

UAS regulatory sequences drive expression of Akt1 which is tagged at the N-terminal end with Tag:MyrPalm(Lck) (myristoylation-palmitoylation motif) and is also tagged with Tag:HA. The resulting Akt1 protein is membrane-anchored.

(Stocker et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Akt
Tagged with
Tag:HA
Tag:MyrPalm(Lck)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larval oenocyte clones expressing Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm-Lck under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C display significantly increased size of oenocytes, as compared to controls.

      (Cinnamon et al., 2016)

      Overexpression of Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1 in fat body cell clones under the control of Scer\GAL4αTub84B.PP leads to a fat body cell overgrowth phenotype.

      (Tiefenböck et al., 2010)

      Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1 with Scer\GAL4GMR.PF induces overgrowth in the eye.

      (Wittwer et al., 2005)

      Flies expressing Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1 under the control of Scer\GAL4GMR.PF have larger eyes than normal due to enlarged ommatidia.

      (Stocker et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      NOT Suppressor of
      Statement
      Reference

      AktUAS.Tag:HA,Tag:MyrPalm(Lck), Scer\GAL4en.PU is a non-suppressor of visible phenotype of PtpmegUAS.cLa, Scer\GAL4en.PU

      (Li et al., 2015)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm-Lck does not suppress the wing vein loss seen when PtpmegScer\UAS.cLa is expressed under the control of Scer\GAL4en.PU.

      (Li et al., 2015)

      The effect of Scer\GAL4αTub84B.PP>Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1 overexpression on cell growth in fat body cell clones is not suppressed by srlKG08646.

      (Tiefenböck et al., 2010)

      Overgrowth in the eye due to Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1 with Scer\GAL4GMR.PF is not suppressed by B4Scer\UAS.cSa.

      (Wittwer et al., 2005)

      The enlarged ommatidia phenotype caused by expression of Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1 under the control of Scer\GAL4GMR.PF is still present in a chicounspecified background.

      (Stocker et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm-Lck
      Akt1Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1
      Akt1UAS.Tag:HA,Tag:MyrPalm(Lck)
      AktACT
      (Otsuki and Brand, 2018)
      AktUAS.Tag:HA,Tag:MyrPalm(Lck)
      Name Synonyms
      Secondary FlyBase IDs
        References (12)