Expression of nos^{αTub84B.3'UTR.UAS} under the control of Scer\GAL4^tj-NP1624 leads to lethality; if the expression is only during adulthood (by the additional control of Gal80[ts] and temperature shift from 18[o]C to 29[o]C), it results in egg chambers with supernumerary germ cells or oocytes.

Animals expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^ppk.PG display severe dendritic arborization defects (loss of terminal branches, decreased total branch length) in class IV dendritic arborizing neurons at third instar larval stage and a significant delay in dendritic pruning of these neurons at the pupal stage.

Over-expression of nos^{αTub84B.3'UTR.Scer\UAS} driven by Scer\GAL4^RapGAP1-OK6 results in boutons with irregular rounded shapes and a slight increase in bouton numbers at the third instar larval neuromuscular junction (NMJ), compared to controls; expression driven by Scer\GAL4^how-24B or Scer\GAL4^C57 produces dramatic changes in NMJ morphology, with abnormal clustering of boutons (not possible to count). Expression driven by Scer\GAL4^RapGAP1-OK6 also results in a significant decrease in the number of active zones in type 1s (but not 1b) boutons. Over-expression of nos^{αTub84B.3'UTR.Scer\UAS} driven by Scer\GAL4^Mhc.PU results in a significant increase in GluRIIA immunoreactivity.

Expression of nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4²²¹ in class I dendritic arborization neurons of third instar larvae does not dramatically change dendrite morphology compared to controls. Expression of nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^109(2)80 results in a dramatic change in the dendrite morphology of class III dendritic arborization neurons of third instar larvae; the number of high-order dendritic branches is significantly reduced while the morphology of the major branches is not affected. Expression of nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4⁴⁷⁷ results in a dramatic change in the dendrite morphology of class IV dendritic arborization neurons of third instar larvae; the number of high-order dendritic branches is significantly reduced while the morphology of the major branches is not affected. Expression of nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^8-123 results in a reduction in the amount of high-order dendritic branches in class IV dendritic arborization neurons of third instar larvae.

When expression is driven by Scer\GAL4^OK107, nos^{αTub84B.3'UTR.Scer\UAS} shows a mushroom body Class III phenotype; axonal growth and guidance defects.

100% of flies expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF have rough eyes. These flies have black spots. 100% of flies expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^hs.2sev have rough eyes. Flies expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^elav.PLu show 82% lethality. Flies expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4¹⁴⁰⁷ show 68% lethality. 26% of flies expressing nos^{αTub84B.3'UTR.Scer\UAS} under the control of Scer\GAL4^hs.2sev show an "adolescent" phenotype; the wing expansion and body cuticle hardening that normally occurs shortly after eclosion fails to occur. The flies are fertile, but show reduced viability. A number of other Scer\GAL4 lines produce this "adolescent" phenotype in combination with nos^{αTub84B.3'UTR.Scer\UAS}, they are: Scer\GAL4^sca-109-68 (74% penetrance), Scer\GAL4^HD23A (85% penetrance), Scer\GAL4^HD44A (90% penetrance), Scer\GAL4^KF42A (38% penetrance) and Scer\GAL4^Eh.2.4 (70% penetrance). Flies carrying nos^{αTub84B.3'UTR.Scer\UAS}, Scer\GAL4^sca-109-68 and Scer\GAL80^αTub84B.PL do not show the adolescent phenotype. Flies carrying nos^{αTub84B.3'UTR.Scer\UAS}, Scer\GAL4^sca-109-68 and Scer\GAL80^Eh.2.4 do show the adolescent phenotype.

Scer\GAL4^sca-109-68, nanos^{αTub84B.3'UTR.UAS} has wing phenotype, non-suppressible by pum⁶⁸⁰

Scer\GAL4^sca-109-68, nanos^{αTub84B.3'UTR.UAS} has adult cuticle phenotype, non-suppressible by pum⁶⁸⁰