Mutation in sequenced strain: deletion (-19, coding/intron).
Rh61 adults exhibit antennal free fluctuations with a diminished power and an altered frequency tuning compared to controls. When stimulated with pure tones, the displacement response of mutant antennae lack the characteristic nonlinear intensity scaling compared to controls. Also the amplitude and sensitivity of compound action potentials responses are reduced in mutants compared to controls. Johnston's organ function of the mutants remain uncompromised by nutrient carotenoid depletion for six generations.
Rh52 homozygous mutant mid and late third instar (96 and 120 hr AEL, respectively) larvae show impaired temperature selection and unlike controls display no strong preference for cooler temperatures (18[o]C) in a temperature gradient. This effect does not appear to be due to developmental delay as the mutants develop with similar timing as controls, they also do not show any gross defects in the morphology of TrpA1 neurons, 18[o]C versus 28[o]C thermotaxis or crawling speed.
Mutant larvae show significantly decreased green-light avoidance compared to controls.
Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. The displacement response of the antenna over a range of sound particle velocities is linearised, indicating loss of mechanical amplification.
Photophobicity is unaffected in Rh61 mutants.
Rh61 mutant class IV dendritic arborization neurons do not exhibit any defects in light response.
Rh61, cryb, norpAP41 has abnormal locomotor rhythm phenotype, enhanceable by Rh52
Rh61, cryb has abnormal locomotor rhythm phenotype, enhanceable by Rh52
Rh61 has abnormal thermotaxis | third instar larval stage phenotype, non-enhanceable by Rh52
Rh61 has abnormal behavior | larval stage phenotype, non-suppressible by Ggt-1Δ1523
Rh61 is an enhancer of abnormal locomotor rhythm phenotype of cryb, norpAP41
Rh52/Rh61 is an enhancer of abnormal locomotor rhythm phenotype of cryb, norpAP41
Rh61 is an enhancer of abnormal locomotor rhythm phenotype of Rh52, cryb, norpAP41
Rh61 is an enhancer of abnormal locomotor rhythm phenotype of Rh52, cryb
Rh52/Rh61 is an enhancer of abnormal locomotor rhythm phenotype of cryb
Rh61 is an enhancer of photoperiod response variant phenotype of ninaE17
Rh61 is an enhancer of photoperiod response variant phenotype of cryb, ninaE17
Rh61 is a non-enhancer of abnormal thermotaxis | third instar larval stage phenotype of Rh52
Rh61 is a non-enhancer of abnormal locomotor rhythm phenotype of cryb
Rh61 is a non-enhancer of abnormal phototaxis phenotype of Rh52
Rh61 is a non-enhancer of photoperiod response variant phenotype of cryb
Rh52, Rh52, Rh61, Rh61 is a suppressor of abnormal circadian rhythm | adult stage phenotype of cry01
Rh61 is a non-suppressor of abnormal phototaxis phenotype of Rh52
Rh52, Rh61 has abnormal neurophysiology | adult stage phenotype
Rh52, Rh61 has abnormal auditory perception | adult stage phenotype
Rh52, Rh61 has abnormal neuroanatomy | third instar larval stage phenotype
Rh52, Rh61, sev1 has abnormal optomotor response phenotype
Rh52, Rh61 has dendrite | third instar larval stage phenotype
Rh52, Rh61 has LNv neuron | third instar larval stage phenotype
Rh61, pros17 has photoreceptor cell R7 phenotype
Rh61, pros17 has photoreceptor cell R8 | ectopic phenotype
The reduced green-light avoidance seen in Rh61 larvae is not altered if the larvae are also mutant for Ggt-1Δ1523.
Following a 6 hour delay in their light-dark cycle, cryb Rh61 flies show a similar delay in resynchronizing their evening activity peak as cryb flies. Triple mutant cryb Rh52 Rh61 flies resynchronize over a longer period than either the cryb single or double mutants.
Following a 6 hour delay in their light-dark cycle, cryb norpA39 flies show a longer delay in resynchronizing their evening activity peak (5-7 days) compared to cryb flies. The addition of Rh61 to this background extends the time required for resynchronization to beyond 7 days. The addition of both Rh52 and Rh61 to the cryb norpA39 background results in minimal (if any) tendency to resynchronize circadian behaviour.
Following a 6 hour advancement in their light-dark cycle, cryb norpA39 flies show a longer delay in resynchronizing their circadian behaviour (several days) compared to either single mutant. Introducing Rh61 to this background interferes more strongly with resynchronization, while the quadruple cryb norpA39 Rh52 Rh61 mutant shows the strongest phenotype.
A Rh61 mutant background does not affect the ability of cryb mutants to adapt to a new phase of green light (472-603nm) and Y-FL (475-724nm).
The retrainment of ninaE17 Rh61 cryb to 6 hour delayed green light (472-603nm) and Y-FL (475-724nm) is significantly slower than that of ninaE17 cryb mutants. Indeed some flies do not adapt to the new photoperiod within 7 days.
ninaE17 Rh53 Rh61 cryb and ninaE17 Rh54 Rh61 cryb quadruple mutants are not retrained by green light (472-603nm) and Y-FL (475-724nm).
Rh61 is rescued by Scer\GAL4TrpA1-AB-G4/Scer\GAL4TrpA1-CD-G4/Rh6UAS.cVa
Rh61/Rh6G is rescued by Scer\GAL4Rh6-G/Rh6UAS.cVa
Rh61 is partially rescued by Scer\GAL4TrpA1-AB-G4/Rh6UAS.cVa
Rh61 is not rescued by Scer\GAL4TrpA1-CD-G4/Rh6UAS.cVa
Expression of Rh6Scer.UAS.cVa in under the control of Scer\GAL4Rh6-G in the Rh61/Rh6G transheterozygote larvae (using the intrinsic Gal4 driver in Rh6G) rescues the impaired thermotactic behavior characteristic for Rh61 mutants. The thermal preferences defects of Rh61 mutants are also rescued by expression of Rh6Scer.UAS.cVa under the combined control of Scer\GAL4TrpA1-AB-G4 and Scer\GAL4TrpA1-CD-G4, the rescue effect is weaker when only the Scer\GAL4TrpA1-AB-G4 driver is used and non-detectable with just the Scer\GAL4TrpA1-CD-G4.
Mutation found during genome annotation of the strain used in the genome sequencing project.