FlyBase Allele Report: Dmel\Krn[s.UAS.cRa]

General Information

Symbol

Dmel\Krn^s.UAS.cRa

Species

D. melanogaster

Name

FlyBase ID

FBal0144768

Feature type

allele

Associated gene

Dmel\Krn

Associated Insertion(s)

Carried in Construct

P{UAS-Krn.s.R}

Also Known As

UAS-sKrn

Key Links

Transgenic product class

stop_gained

(Reich and Shilo, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Reich and Shilo, 2002)

Mutagen

in vitro construct

(Reich and Shilo, 2002)

Progenitor genotype

Carried in construct

P{UAS-Krn.s.R}

Cytology

Description

A predicted secreted form of Krn (a stop codon has been introduced at amino acid residue 113) is expressed under the control of UASt regulatory sequences.

(Reich and Shilo, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Krn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | third instar larval stage, with Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP0001

(Jiang et al., 2011)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP7397

(Jiang et al., 2011)

lethal, with Scer\GAL4^69B

(Reich and Shilo, 2002)

partially lethal - majority die, with Scer\GAL4^Bx-MS1096

(Reich and Shilo, 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Krn^{s.Scer\UAS.cRa} in the midgut, under the control of Scer\GAL4^esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.

Expression of Krn^{s.Scer\UAS.cRa} in mature enterocytes, under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B results in the generation of many new midgut cells, including enterocyte-like cells.

(Jiang et al., 2011)

Induction of Krn^{s.Scer\UAS.cRa} by Scer\GAL4^esg-NP7397 results in extensive overproliferation of adult midgut progenitor cells, while limiting their dispersal, thus reducing the number of AMP clusters in wandering third instar larval midguts.

(Jiang and Edgar, 2009)

Rare Krn^{s.Scer\UAS.cRa}; Scer\GAL4^Bx-MS1096 adults have short bloated stumps for wings. Krn^{s.Scer\UAS.cRa}; Scer\GAL4^55B flies lay eggs with ectopic dorsal appendages. The cuticles of Krn^{s.Scer\UAS.cRa}; Scer\GAL4^69B embryos have denticle belts which are wider, extend more dorsally and are more square edged than wild-type. These cuticles lack head structures, and have a phenotype indicative of defective germ-band retraction.

(Reich and Shilo, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Krn^s.UAS.cRa, Scer\GAL4^NP7397 has increased occurrence of cell division | adult stage phenotype, non-suppressible by dome^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Jiang et al., 2011)

Krn^s.UAS.cRa, Scer\GAL4^NP7397 has increased occurrence of cell division | adult stage phenotype, non-suppressible by Stat92E^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Jiang et al., 2011)

Suppressor of

Statement

Reference

Krn^s.UAS.cRa, Scer\GAL4^NP0001 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP0001, upd1^UAS.cUa

(Jiang et al., 2011)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Krn^s.UAS.cRa, Scer\GAL4^NP7397, Scer\GAL80^ts.αTub84B has intestinal stem cell phenotype, non-suppressible by dome^RNAi.UAS.cUa, Scer\GAL4^NP7397, Scer\GAL80^ts.αTub84B

(Jiang et al., 2011)

Krn^s.UAS.cRa, Scer\GAL4^NP7397, Scer\GAL80^ts.αTub84B has intestinal stem cell phenotype, non-suppressible by Stat92E^RNAi.UAS.cUa, Scer\GAL4^NP7397, Scer\GAL80^ts.αTub84B

(Jiang et al., 2011)

Suppressor of

Statement

Reference

Krn^s.UAS.cRa, Scer\GAL4^NP0001 is a suppressor of intestinal stem cell | adult stage phenotype of Scer\GAL4^NP0001, upd1^UAS.cUa

(Jiang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A Stat92E^{dsRNA.Scer\UAS.cUa} or dome^{dsRNA.Scer\UAS.cUa} background does not affect the stimulatory effect of Krn^{s.Scer\UAS.cRa} expression (under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B) on intestinal stem cell proliferation.

(Jiang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Krn^{s.Scer\UAS.cRa}

Krn^s.UAS.cRa

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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