FlyBase Allele Report: Scer\GAL4[55B]

General Information

Symbol

Scer\GAL4^55B

Species

S. cerevisiae

Name

FlyBase ID

FBal0279486

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}Eip63F-1^55B

Carried in Construct

Also Known As

55B-GAL4, 55B, 55B GAL4

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Brand, 1997.6.30, Queenan et al., 1997, Brand and Perrimon, 1994)

Progenitor genotype

Associated Insertion(s)

P{GawB}Eip63F-1^55B

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:3,926,447..3,926,447

(Hartman et al., 2015, Wang et al., 2015, Niepielko et al., 2012, Zappia et al., 2011, Skora and Spradling, 2010, Zhang et al., 2009, Furriols et al., 2007, Carneiro et al., 2006, Goentoro et al., 2006, Morris et al., 2006, Pai et al., 2006, Wahlstrom et al., 2006, Acebes et al., 2004, Meignin et al., 2004, Ghiglione et al., 2002, Halfon et al., 2002, Reich and Shilo, 2002, Grammont and Irvine, 2001, Johnson Hamlet and Perkins, 2001, Kiger and Ho, 2001, Suzanne et al., 2001, Yoshida et al., 2001, Araujo and Bier, 2000, Jordan et al., 2000, Bryant et al., 1999, Mantrova et al., 1999, Reich et al., 1999, Sureau and Ferveur, 1999, Mantrova and Hsu, 1998, Sapir et al., 1998, Brand, 1997.6.30, Ferveur et al., 1997, Nakayama et al., 1997, Queenan et al., 1997, Ferveur and Sureau, 1996, Morimoto et al., 1996, Ferveur et al., 1995, Brand and Perrimon, 1994, Brand et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Ferveur et al., 1995)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval salivary gland

(Brand and Perrimon, 1994)

adult stage

adult midgut

(Ferveur et al., 1997)

crop

(Ferveur et al., 1997)

adult stage & oogenesis stage S9

follicle cell

(Brand and Perrimon, 1994)

adult stage oogenesis stage S8 -- S14

follicle cell | anterior

(Queenan et al., 1997)

oogenesis

follicle cell | anterior

(Pai et al., 2006)

centripetal follicle cell

(Furriols et al., 2007)

oogenesis stage S10A

follicle cell

(Goentoro et al., 2006)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

adult head

(Nakayama et al., 1997)

adult thorax

(Nakayama et al., 1997)

Additional Information

Statement

Reference

Drives expression in anterior follicle cells starting at oogenesis stage S8.

(FlyBase Curators, 2017)

ScerGAL4^55B drives expression in anterior follicle cells.

(Pai et al., 2006)

Head, thoracic and abdominal extracts of virgin females were subjected to Western analysis, using β-gal antibodies.

(Nakayama et al., 1997)

During oogenesis, from stage S8, expressed in follicle cells over the anterior portion of the oocyte.

(Queenan et al., 1997)

ScerGAL4 expression occurs in a stripeof follicle cells around the anterior of the oocyte in a stage S9 eggchamber. Embryonic ScerGAL4 expression is limited to the salivary glands.

(Brand and Perrimon, 1994)

Expression in a stripe of follicle cells around the anterior of the oocyte in stage 9 egg chambers.

(Brand and Perrimon, 1994)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult nervous system | male, with tra^UAS.cFa

(Acebes et al., 2004)

dorsal appendage, with Cbl^L.UAS

(Pai et al., 2006)

dorsal appendage, with Egfr^DN.UAS.cBa

(Wahlstrom et al., 2006)

dorsal appendage, with pnt^P1.UAS

(Morimoto et al., 1996)

dorsal appendage, with puc^UAS.cMa

(Suzanne et al., 2001)

dorsal appendage, with spi^s.UAS

(Sapir et al., 1998)

dorsal appendage | ectopic, with btl::Egfr^λ.UAS

(Queenan et al., 1997)

dorsal appendage | ectopic, with Krn^s.UAS.cRa

(Reich and Shilo, 2002)

dorsal appendage | ectopic, with Krn^UAS.cRa

(Reich and Shilo, 2002)

dorsal appendage | ectopic, with rho^UAS.cGa

(Sapir et al., 1998)

dorsal appendage | maternal effect, with Hsap\RAF1^gof.UAS

(Brand and Perrimon, 1994)

dorsal appendage | maternal effect, with Raf^UAS.F179

(Brand and Perrimon, 1994)

egg, with btl::Egfr^λ.UAS

(Queenan et al., 1997)

egg, with Cbl^L.UAS

(Pai et al., 2006)

egg chorion, with Cbl^L.UAS

(Pai et al., 2006)

egg chorion | maternal effect, with Hsap\RAF1^gof.UAS

(Brand and Perrimon, 1994)

egg chorion | maternal effect, with Raf^UAS.F179

(Brand and Perrimon, 1994)

egg operculum | maternal effect | somatic clone, with dpp^UAS.E.EGFP

(Carneiro et al., 2006)

embryo, with csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

embryo | maternal effect, with Hsap\RAF1^gof.UAS

(Brand and Perrimon, 1994)

embryo | maternal effect, with Raf^UAS.F179

(Brand and Perrimon, 1994)

embryonic/first instar larval cuticle | anterior, with btl::Egfr^λ.UAS

(Queenan et al., 1997)

embryonic epidermis, with dpp^UAS.cHa

(Araujo and Bier, 2000)

follicle cell, with awd^RNAi.UAS

(Woolworth et al., 2009)

follicle cell, with awd^UAS.cDa

(Woolworth et al., 2009)

follicle cell, with Rab5^{Q88L.UASp.YFP}

(Woolworth et al., 2009)

follicle cell, with Rab5^{S43N.UASp.YFP}

(Woolworth et al., 2009)

follicle cell, with Rab5^UASp.YFP

(Woolworth et al., 2009)

follicle cell | adult stage, with Ctps^GD4740

(Wang et al., 2015)

follicle cell | maternal effect, with Hsap\RAF1^gof.UAS

(Brand and Perrimon, 1994)

follicle cell | maternal effect, with Raf^UAS.F179

(Brand and Perrimon, 1994)

larval dorsal hair, with rho^UAS.cGa

(Sapir et al., 1998)

larval dorsal hair, with spi^s.UAS

(Sapir et al., 1998)

micropyle, with puc^UAS.cMa

(Suzanne et al., 2001)

ventral denticle belt, with dpp^UAS.cHa

(Araujo and Bier, 2000)

ventral denticle belt, with rho^UAS.cGa

(Sapir et al., 1998)

ventral denticle belt, with spi^s.UAS

(Sapir et al., 1998)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, non-enhanceable by Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B

(Woolworth et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, suppressible | partially by Rab5^{Q88L.UASp.YFP}, Scer\GAL4^55B

(Woolworth et al., 2009)

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, suppressible | partially by Rab5^UASp.YFP, Scer\GAL4^55B

(Woolworth et al., 2009)

Scer\GAL4^55B, rho^UAS.cGa has larval dorsal hair phenotype, suppressible | partially by grk⁶

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has dorsal appendage phenotype, suppressible by S^IIN

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has ventral denticle belt phenotype, suppressible by S^IIN

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has larval dorsal hair phenotype, suppressible by S^IIN

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has dorsal appendage phenotype, suppressible | partially by grk³

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has ventral denticle belt phenotype, suppressible | partially by grk³

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has larval dorsal hair phenotype, suppressible | partially by grk³

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has dorsal appendage phenotype, suppressible | partially by grk⁶

(Sapir et al., 1998)

Scer\GAL4^55B, rho^UAS.cGa has ventral denticle belt phenotype, suppressible | partially by grk⁶

(Sapir et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, non-suppressible by Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B

(Woolworth et al., 2009)

NOT Enhancer of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B is a non-enhancer of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

Suppressor of

Statement

Reference

Rab5^{Q88L.UASp.YFP}, Scer\GAL4^55B is a suppressor | partially of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

Rab5^UASp.YFP, Scer\GAL4^55B is a suppressor | partially of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

Scer\GAL4^55B/Shark^UAS.cFa is a suppressor | partially of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

NOT Suppressor of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B is a non-suppressor of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

1803

w^*; P{GawB}Eip63F-1^55B

Kyoto

106513

w^*; P{GawB}Eip63F-1^55B

Notes on Origin

Discoverer

Brand and Perrimon

(Brand and Perrimon, 1994)

Comments

Activates Ecol\lacZ^Scer\UAS expression in a stripe of follicle cells around the anterior of the oocyte in a stage 9 egg chamber.

(Brand and Perrimon, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^55B

(Woolworth et al., 2009)

Scer\GAL4^55B

Scer\GAL4^noeF

Name Synonyms

Secondary FlyBase IDs

FBal0040467
FBal0047048
FBal0191262

References (43)

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