FlyBase Allele Report: Dmel\Ctps[GD4740]

General Information

Symbol

Dmel\Ctps^GD4740

Species

D. melanogaster

Name

FlyBase ID

FBal0205229

Feature type

allele

Associated gene

Dmel\Ctps

Associated Insertion(s)

Carried in Construct

P{GD4740}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4740}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4740

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | adult stage, with Scer\GAL4^55B

(Wang et al., 2015)

abnormal endomitotic cell cycle | adult stage, with Scer\GAL4^CY2

(Wang et al., 2015)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval lymph gland, with Scer\GAL4^HCH.Hand, Scer\GAL4^Hml.Δ

(Mondal et al., 2014)

embryonic/larval lymph gland, with Scer\GAL4^Hml.Δ

(Mondal et al., 2014)

follicle cell | adult stage, with Scer\GAL4^55B

(Wang et al., 2015)

follicle cell | adult stage, with Scer\GAL4^CY2

(Wang et al., 2015)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

nucleus | adult stage, with Scer\GAL4^CY2

(Wang et al., 2015)

Detailed Description

Statement

Reference

The expression of CTPsyn^GD4740 under the control of Scer\GAL4^c564 has no obvious affect on the inflammatory response to Gram-positive bacterial infection or under basal conditions, as compared to controls.

(Kumar et al., 2016)

Expression of CTPsyn^GD4740 RNAi (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4^55B or Scer\GAL4^CY2 results in significant decrease in the proportion of follicle cells undergoing DNA replication replication (measured by BrU incorporation) in stage 9 egg chambers compared to controls. Expression under the Scer\GAL4^CY2 driver also leads to reduced nuclear size in stage 10A egg chambers compared to controls.

(Wang et al., 2015)

Expression via "HHLT-gal4" or Scer\GAL4^Hml.Δ results in loss of progenitor cells in the primary lobes of the larval lymph gland. ("HHLT-gal4" is a combination of Scer\GAL4^HCH.Hand and Scer\GAL4^Hml.Δ that express in early lymph gland blood cells (hemocytes) and circulating and sessile blood cells, respectively, and incorporates Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} to maintain expression in the lymph gland after Scer\GAL4^HCH.Hand is down-regulated.)

(Mondal et al., 2014)

No wing phenotypes are evident when CTPsyn^GD4740 is expressed under the control of Scer\GAL4^Bx-MS1096.

(Shukla et al., 2014)

Knockdown of CTPsyn, through expression of CTPsyn^GD4740, under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}, does not impede clonal growth.

(Willoughby et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls (depending on the insertion line used).

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in mild defects in the shape of ganglion mother cells in the larval brain (depending on the insertion line used).

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in an increase in the average size of ganglion mother cells in the larval brain compared to controls (depending on the insertion line used).

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in mild defects in the shape of neuroblasts in the larval brain (depending on the insertion line used).

(Neumüller et al., 2011)

Adults expressing CTPsyn^GD4740 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4740

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ctps^GD4740, Scer\GAL4^CY2 has abnormal endomitotic cell cycle | adult stage phenotype, enhanceable by Cbl^F165/Cbl[+]

(Wang et al., 2015)

Enhancer of

Statement

Reference

Ctps^GD4740, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

Suppressor of

Statement

Reference

Ctps^GD4740, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of neoplasia phenotype of Ras85D^V12.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, scrib¹

(Willoughby et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ctps^GD4740, Scer\GAL4^CY2 has follicle cell | adult stage phenotype, enhanceable by Cbl^F165/Cbl[+]

(Wang et al., 2015)

Ctps^GD4740, Scer\GAL4^CY2 has nucleus | adult stage phenotype, enhanceable by Cbl^F165/Cbl[+]

(Wang et al., 2015)

Enhancer of

Statement

Reference

Ctps^GD4740, Scer\GAL4^Bx-MS1096 is an enhancer of wing hinge phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

Other

Statement

Reference

Cbl^F165, Ctps^GD4740, Scer\GAL4^CY2 has egg phenotype

(Wang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The decrease in percentage of follicle cells undergoing DNA replication (measured by BrdU incorporation) observed in stage 9 egg chambers as well as the decreased follicle cell nuclear size in stage 10A egg chamber expressing CTPsyn^GD4740 under the control of Scer\GAL4^CY2 is further decreased by combination with Cbl^F165 in heterozygous state.

About a fifth of eggs laid by Cbl^F165 heterozygous females expressing CTPsyn^GD4740 under the control of Scer\GAL4^CY2 has a defective collapsed phenotype that is not observed in eggs laid by Cbl^F165/+ flies.

(Wang et al., 2015)

Expression of CTPsyn^GD4740 enhances the wing hinge phenotype seen when hng1^KK103642 is expressed under the control of Scer\GAL4^Bx-MS1096.

(Shukla et al., 2014)

Knockdown of CTPsyn, through expression of CTPsyn^GD4740, induces a strong, significant reduction in tumor growth in Ras85D^V12.Scer\UAS-scrib¹ mutant larval MARCM clones.

(Willoughby et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v12759