FB2024_03 , released June 25, 2024
Allele: Dmel\Rap1V12.UAS.Tag:MYC
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General Information
Symbol
Dmel\Rap1V12.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0152266
Feature type
allele
Associated gene
Dmel\Rap1
Associated Insertion(s)
Carried in Construct
P{UAS-Rap1.V12}
Also Known As
UAS-rap1V12
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Boettner et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-Rap1.V12}
Cytology
Description

UASt regulatory sequences drive expression of a constitutively active form of Rap1 that is tagged between the 2nd and 3rd amino acid of Rap1 with Tag:MYC.

(Boettner et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rap1
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of RV12.Scer\UAS.T:Hsap\MYC in macrophages, under the control of Scer\GAL4srp, results in a significant increase in the size of cellular protrusions from these macrophages, a change in macrophage shape, and the formation of a dense set of cells along the dorsal edge of the epidermis.

      (Huelsmann et al., 2006)

      Animals expressing RV12.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1 do not hatch. 90% of the embryos have their cuticle closed dorsally but show a head involution defect and 10% have the cuticle partially open.

      (Boettner et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4ptc-559.1/Rap1V12.UAS.Tag:MYC is a non-suppressor of lethal | embryonic stage phenotype of cno2

      (Boettner et al., 2003)
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      Statement
      Reference

      Rap1V12.UAS.Tag:MYC/Scer\GAL4upd1.PU is a suppressor of cell-cell adherens junction & hub cell phenotype of PDZ-GEF6/PDZ-GEF4

      (Wang et al., 2006)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4ptc-559.1/Rap1V12.UAS.Tag:MYC is a non-suppressor of embryo | dorsal closure stage phenotype of cno2

      (Boettner et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The macrophage phenotype of Scer\GAL4srp>RV12.Scer\UAS.T:Hsap\MYC embryos is suppressed in a mys11 background; these macrophages show no enlargement of protrusions, or cell shape change.

      (Huelsmann et al., 2006)

      RV12.Scer\UAS.T:Hsap\MYC; Scer\GAL4upd rescues the formation of adherens junctions between male germline stem cells and hub cells in the germinal proliferation center of Gef264/Gef266 animals.

      (Wang et al., 2006)

      The dorsal closure defects seen in cno2 embryos are not rescued by co-expression of expressing RV12.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1.

      (Boettner et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      RV12.Scer\UAS.T:Hsap\MYC
      Rap1V12.Scer\UAS.T:Hsap\MYC
      Rap1V12.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (9)