FlyBase Allele Report: Scer\GAL4[upd1.PU]

General Information

Symbol

Scer\GAL4^upd1.PU

Species

S. cerevisiae

Name

unpaired 1 promoter construct of Unknown

FlyBase ID

FBal0144580

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{upd1-GAL4.U}

Also Known As

Upd-Gal4, updGal4, upd1-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Bai and Montell, 2002)

Progenitor genotype

Carried in construct

P{upd1-GAL4.U}

Cytology

Description

upd1 regulatory sequences control expression of a GAL4 driver.

(FlyBase, 1992-)

Allele components

Component

Use(s)

Regulatory region(s)

upd1

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

gonadal sheath proper primordium

(Le Bras and Van Doren, 2006)

adult stage

hub cell

(Le Bras and Van Doren, 2006)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

hub cell

(Lee et al., 2016)

adult stage | male & spermatogenesis

hub cell

(Fairchild et al., 2016)

Additional Information

Statement

Reference

The upd1 reporter is expressed in the anterior cluster of somatic gonadal precursor cells in the stage 17 embryonic gonad. It is also expressed in the adult hub.

(Le Bras and Van Doren, 2006)

Marker for

hub cell

(Fairchild et al., 2016, Lee et al., 2016)

polar follicle cell

(Chang et al., 2013)

Reflects expression of

upd1

(Lee et al., 2016, Chang et al., 2013, Le Bras and Van Doren, 2006)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | oogenesis, with raskol^HMS03717

(Raza et al., 2019)

abnormal cell adhesion | oogenesis, with shg^HMS00693

(Raza et al., 2019)

abnormal cell migration | adult stage | female, with Burs^JF02260, Scer\GAL4^slbo.2.6

(Anllo and Schüpbach, 2016)

decreased cell number | adult stage, with CG46339^GD11680

(Lim et al., 2015)

decreased cell number | adult stage, with shg^{dNc.UAS.sgGFP}

(Lim et al., 2015)

decreased cell number | oogenesis, with eya^UAS.cBa

(Bai and Montell, 2002)

Phenotype Manifest In

border follicle cell, with BicD^GD11974

(Van De Bor et al., 2011)

border follicle cell, with BicD^KK100487

(Van De Bor et al., 2011)

border follicle cell, with Burs^JF02260, Scer\GAL4^slbo.2.6

(Anllo and Schüpbach, 2016)

border follicle cell, with DCTN1-p150^GD1455

(Van De Bor et al., 2011)

border follicle cell, with egl^KK105186

(Van De Bor et al., 2011)

border follicle cell, with Lis-1^GD1480

(Van De Bor et al., 2011)

border follicle cell, with Rala^S25N.UAS, Scer\GAL4^slbo.2.6

(Ghiglione et al., 2008)

border follicle cell, with Rap1^{N17.UAS.Tag:MYC}, Scer\GAL4^slbo.2.6

(Ghiglione et al., 2008)

border follicle cell, with raskol^HMS03717

(Raza et al., 2019)

border follicle cell, with Scer\GAL4^slbo.2.6, baz^RNAi.Sym.UAS

(Pinheiro and Montell, 2004)

border follicle cell, with Scer\GAL4^slbo.2.6, par-6^RNAi.Sym.UAS

(Pinheiro and Montell, 2004)

border follicle cell, with shg^HMS00693

(Raza et al., 2019, Cai et al., 2014)

border follicle cell, with spas^UAS.EGFP

(Van De Bor et al., 2011)

border follicle cell, with upd1^GD1158

(Van De Bor et al., 2011)

border follicle cell & filopodium | ectopic, with Scer\GAL4^slbo.2.6, par-6^RNAi.Sym.UAS

(Pinheiro and Montell, 2004)

cyst progenitor cell | adult stage, with CG46339^GD11680

(Lim et al., 2015)

cyst progenitor cell | adult stage, with shg^{dNc.UAS.sgGFP}

(Lim et al., 2015)

egg chamber & basement membrane | apical, with eya^UAS.cBa

(Medioni and Noselli, 2005)

egg chamber & basement membrane | apical, with eya^UAS.cBb

(Medioni and Noselli, 2005)

egg chamber | anterior, with emc^EP3620

(Adam and Montell, 2004)

filamentous actin | oogenesis, with upd1^GD1158

(Mallart et al., 2024)

hub cell | adult stage, with CG46339^GD11680

(Lim et al., 2015)

hub cell | adult stage, with shg^{dNc.UAS.sgGFP}

(Lim et al., 2015)

hub cell | progressive, with shv^UAS.Tag:HA

(Lee et al., 2016)

male fusome, with CG46339^UASp.Tag:HA

(Lim et al., 2015)

male germline stem cell | adult stage, with CG46339^GD11680

(Lim et al., 2015)

male germline stem cell | adult stage, with shg^{dNc.UAS.sgGFP}

(Lim et al., 2015)

male germline stem cell | progressive, with shv^UAS.Tag:HA

(Lee et al., 2016)

oocyte, with emc^EP3620

(Adam and Montell, 2004)

oocyte, with upd1^GD1158

(Mallart et al., 2024)

perivitelline space, with upd1^GD1158

(Mallart et al., 2024)

polar follicle cell, with emc^EP3620

(Adam and Montell, 2004)

polar follicle cell, with eya^UAS.cBa

(Medioni and Noselli, 2005, Bai and Montell, 2002)

polar follicle cell, with eya^UAS.cBb

(Medioni and Noselli, 2005)

polar follicle cell | increased number, with emc^EP3620

(Adam and Montell, 2004)

spermatocyte | spermatogenesis, with AGO1^HMS00610

(Bozzetti et al., 2015)

spermatocyte | spermatogenesis, with Fmr1^GD1288

(Bozzetti et al., 2015)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has decreased cell number | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

Suppressor of

Statement

Reference

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

NOT Suppressor of

Statement

Reference

upd1^UAS.cZa, Scer\GAL4^upd1.PU is a non-suppressor of decreased cell number | adult stage phenotype of CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU

(Lim et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^upd1.PU, upd1^GD1158 has oocyte phenotype, suppressible by shg^UAS.cUa/Scer\GAL4^upd1.PU

(Mallart et al., 2024)

Scer\GAL4^upd1.PU, upd1^GD1158 has perivitelline space phenotype, suppressible by shg^UAS.cUa/Scer\GAL4^upd1.PU

(Mallart et al., 2024)

NOT suppressed by

Statement

Reference

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has hub cell | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has male germline stem cell | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU has cyst progenitor cell | adult stage phenotype, non-suppressible by upd1^UAS.cZa, Scer\GAL4^upd1.PU

(Lim et al., 2015)

Suppressor of

Statement

Reference

shg^UAS.cUa/Scer\GAL4^upd1.PU is a suppressor of oocyte phenotype of Scer\GAL4^upd1.PU, upd1^GD1158

(Mallart et al., 2024)

shg^UAS.cUa/Scer\GAL4^upd1.PU is a suppressor of perivitelline space phenotype of Scer\GAL4^upd1.PU, upd1^GD1158

(Mallart et al., 2024)

aub^UAS.cBa/Scer\GAL4^upd1.PU is a suppressor of spermatocyte | spermatogenesis phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor | partially of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor | partially of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.sgGFP/Scer\GAL4^upd1.PU is a suppressor of cell-cell adherens junction & hub cell phenotype of PDZ-GEF³/PDZ-GEF⁴

(Wang et al., 2006)

Rap1^{V12.UAS.Tag:MYC}/Scer\GAL4^upd1.PU is a suppressor of cell-cell adherens junction & hub cell phenotype of PDZ-GEF⁶/PDZ-GEF⁴

(Wang et al., 2006)

NOT Suppressor of

Statement

Reference

aub^UAS.cBa, aub[+], aub^HN2, Scer\GAL4^upd1.PU is a non-suppressor of spermatocyte | spermatogenesis phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a non-suppressor of male fusome phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

upd1^UAS.cZa, Scer\GAL4^upd1.PU is a non-suppressor of hub cell | adult stage phenotype of CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU

(Lim et al., 2015)

upd1^UAS.cZa, Scer\GAL4^upd1.PU is a non-suppressor of male germline stem cell | adult stage phenotype of CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU

(Lim et al., 2015)

upd1^UAS.cZa, Scer\GAL4^upd1.PU is a non-suppressor of cyst progenitor cell | adult stage phenotype of CG46339^{E-A.UASp.Tag:HA}, Df(3R)ED6235/jus^iso7.8, Scer\GAL4^upd1.PU

(Lim et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Fmr1^UAS.cZa/Scer\GAL4^upd1.PU rescues Fmr1^Δ113M/Fmr1[+]

(Bozzetti et al., 2015)

CG46339^UASp.Tag:HA/Scer\GAL4^upd1.PU rescues Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

magu^UAS.Tag:V5/Scer\GAL4^upd1.PU rescues magu^e00439/magu^f02256

(Zheng et al., 2011)

Fails to rescue

shv^UAS.Tag:HA/Scer\GAL4^upd1.PU fails to rescue shv¹

(Lee et al., 2016)

CG46339^{E-A.UASp.Tag:HA}/Scer\GAL4^upd1.PU fails to rescue Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CG46339^{ΔCAT.UASp.Tag:HA}/Scer\GAL4^upd1.PU fails to rescue Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Scer\GAL4^upd.PU

Scer\GAL4^upd1.PU

Scer\GAL4^upd

Name Synonyms

unpaired 1 promoter construct of Unknown

unpaired construct of Unknown

Secondary FlyBase IDs

References (45)

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