FlyBase Allele Report: Dmel\tws[UAS.cBa]

General Information

Symbol

Dmel\tws^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Bajpai

FlyBase ID

FBal0158539

Feature type

allele

Associated gene

Dmel\tws

Associated Insertion(s)

Carried in Construct

P{UAS-tws.B}

Also Known As

UAS-Tws

Key Links

Transgenic product class

cDNA

(Bajpai et al., 2004)

wild_type

(Bajpai et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Bajpai et al., 2004)

wild_type

(Bajpai et al., 2004)

Mutagen

in vitro construct

(Bajpai et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-tws.B}

Cytology

Description

Expression of full length tws cDNA (LD12394) is under the control of UAS sequences.

(Bajpai et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tws

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autosomal dominant cerebellar ataxia

CEA

(Wang et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of autosomal dominant cerebellar ataxia

is ameliorated by Sod2^UAS.cMa

(Wang et al., 2011)

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Yeh et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^elav.PU

(Wang et al., 2011)

increased cell death | embryonic stage, with Scer\GAL4^Act5C.PU

(Wang et al., 2011)

increased cell death | embryonic stage, with Scer\GAL4^elav.PU

(Wang et al., 2011)

short lived | heat sensitive, with Scer\GAL4^elav.PU

(Wang et al., 2011)

Phenotype Manifest In

adult brain | progressive, with Scer\GAL4^elav.PU

(Wang et al., 2011)

mitochondrion, with Scer\GAL4^elav.PU

(Wang et al., 2011)

mitochondrion, with Scer\GAL4^en.PU

(Wang et al., 2011)

wing margin bristle | ectopic, with Scer\GAL4^hs.PB, tws⁶⁰

(Bajpai et al., 2004)

Detailed Description

Statement

Reference

Expression of tws^Scer\UAS.cBa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^Act5C.PU results in a large number of apoptotic cells in embryos.

Expression of tws^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU reduces the eclosion rate to approximately 40%.

Flies expressing tws^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU at 29[o]C show a severe reduction in lifespan compared to controls.

Newly eclosed adults expressing tws^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU at 29[o]C do not show abnormalities in the brain. However, 7 day old flies show vacuolisation of both cortex and neuropil.

Mitochondrial size is reduced and mitochondrial density is increased in the axons of the larval ventral ganglia in animals expressing tws^Scer\UAS.cBa under the control of Scer\GAL4^en.PU. The mitochondrial reticulum is disintegrated into multiple punctiform organelles.

Mitochondria of photoreceptor neurons are considerably smaller than controls in flies expressing tws^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU. Scission of mitochondria is seen. Internal cristae of disintegrated mitochondria are less condensed than normal and some are circled by autophagosome-like organelles.

Mitochondrial membrane potential is significantly reduced in the heads of flies expressing tws^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU.

Flies expressing tws^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU at 29[o]C and treated with 20mM paraquat show reduced climbing ability compared to control flies.

(Wang et al., 2011)

tws^Scer\UAS.cBa; Scer\GAL4^hs.PB; tws⁶⁰/tws⁶⁰ animals have scattered ectopic bristles on their wing blades.

(Bajpai et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, tws^UAS.cBa has short lived | heat sensitive phenotype, suppressible by Scer\GAL4^elav.PU/Sod2^UAS.cMa

(Wang et al., 2011)

Suppressor of

Statement

Reference

tws^UAS.cBa, Scer\GAL4^ppk.PG is a suppressor of abnormal neuroanatomy | larval stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^ppk.PG

(Yeh et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

tws^UAS.cBa, Scer\GAL4^Eq is a suppressor of macrochaeta phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^Eq

(Yeh et al., 2010)

tws^UAS.cBa, Scer\GAL4^Eq is a suppressor of sensory organ cell phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^Eq

(Yeh et al., 2010)

tws^UAS.cBa, Scer\GAL4^ppk.PG is a suppressor of neurite phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^ppk.PG

(Yeh et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Sod2^Scer\UAS.cMa extends the lifespan of flies expressing tws^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU at 29[o]C.

(Wang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of tws^Scer\UAS.cBa mitigates the Hsap\MAPT^Scer\UAS.cWa-induced bristle loss phenotype (with both under the control of Scer\GAL4^Eq1). Bristle and sensory cell numbers increase concurrently when tws^Scer\UAS.cBa is co-expressed with Hsap\MAPT^Scer\UAS.cWa.

Expression of tws^Scer\UAS.cBa in larval da neurons (under the control of Scer\GAL4^ppk.PG) suppresses the aggregate formation and dysmorphology of neurites found upon expression of Hsap\MAPT^Scer\UAS.cWa.

(Yeh et al., 2010)

Complementation and Rescue Data

Partially rescues

tws^UAS.cBa/Scer\GAL4^hs.PB partially rescues tws⁶⁰

(Bajpai et al., 2004)

tws^UAS.cBa/Scer\GAL4^en-e16E partially rescues tws^j11C8

(Bajpai et al., 2004)

Fails to rescue

tws^UAS.cBa/Scer\GAL4^hs.PB fails to rescue tws^j11C8

(Bajpai et al., 2004)

Comments

tws^Scer\UAS.cBa; Scer\GAL4^hs.PB rescues survival of tws⁶⁰ to the pharate adult stage, with no obvious morphological defects apart from scattered ectopic bristles on the wing blades. The larval/pupal lethality of tws^j11C8 homozygotes is not rescued by tws^Scer\UAS.cBa; Scer\GAL4^hs.PB, but is partially rescued by tws^Scer\UAS.cBa; Scer\GAL4^en-e16E.

(Bajpai et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tws^Scer\UAS.cBa

tws^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Bajpai

(Bajpai et al., 2004)

Secondary FlyBase IDs

References (3)

Report Sections

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