FlyBase Allele Report: Dmel\SERCA[Kum170]

General Information

Symbol

Dmel\SERCA^Kum170

Species

D. melanogaster

Name

FlyBase ID

FBal0184441

Feature type

allele

Associated gene

Dmel\SERCA

Associated Insertion(s)

Carried in Construct

Also Known As

Kum¹⁷⁰, CaP60A^Kum170, Ca-P60A^Kum170

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Sanyal et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: E442K.

(Sanyal et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,931,225..23,931,225 [-]

Nucleotide change:

G23931225A

Amino acid change:

Reported amino acid change:

E442K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Sanyal et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response | dominant

(Abraham and Wolf, 2013)

abnormal neuroanatomy | dominant | third instar larval stage

(Wong et al., 2014)

abnormal neurophysiology | heat sensitive

(Sanyal et al., 2005, Hoeffer et al., 2003)

abnormal neurophysiology | larval stage

(Freeman et al., 2010)

decreased cell size | somatic clone

(Bi et al., 2014)

decreased rate of movement | larval stage | recessive

(Sanyal et al., 2005)

lethal (with Df(2R)BSC601)

(Kahsai et al., 2016)

lethal (with SERCA^Kum295)

(Sanyal et al., 2005)

lethal (with SERCA^KumP52)

(Sanyal et al., 2005)

lethal | recessive

(Sanyal et al., 2005)

paralytic | adult stage | dominant | heat sensitive

(Sanyal et al., 2005)

paralytic | dominant

(Abraham and Wolf, 2013)

paralytic | dominant | larval stage | heat sensitive

(Sanyal et al., 2005)

paralytic | heat sensitive

(Balasubramanian and Srinivasan, 2020, Hoeffer et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

SERCA^Kum170 heterozygotes do not show flight defects.

(Balasubramanian and Srinivasan, 2020)

Ca-P60A^Kum170 mutant fat body cell clones have fewer and smaller lipid droplets than adjacent control cells. In addition, the mutant cells are smaller than the surrounding wild type cells. The salivary glands in Ca-P60A^Kum170 mutants are similar in size to wild type.

(Bi et al., 2014)

Unlike in wild type, all heterozygous Ca-P60A^Kum170 mutant flies subjected to 7 or 10 minute heat shocks develop paralysis. 20% of flies exposed to a five minute heat shock undergo paralysis. The flies that develop paralysis do not recover normal function until 72 hours after shock. Heat rate is reduced and is inversely proportional to the length of the heat shock. End diastolic dimension is increased but there is no effect on fractional shortening.

(Abraham and Wolf, 2013)

Synapse strength (neurotransmitter release per action potential) is increased compared to wild type at muscle 6/7 neuromuscular junctions in mutant larvae.

(Freeman et al., 2010)

Larval neurons heterozygous for Ca-P60A^Kum170 exhibit elevated levels of basal cytosolic Ca[2+].

(Venkiteswaran and Hasan, 2009)

After depolarisation with 70mM KCl, neurons cultured from Ca-P60A^Kum170/+ larvae return to basal intracellular Ca[2+] levels is slower than in neurons cultured from wild-type larvae.

(Banerjee et al., 2006)

Heterozygous adults paralyse within 3 minutes between 39^o and 40^oC. Heterozygous larvae also paralyse at 40^oC within 5 minutes and the paralysed larvae appear shortened due to severe contraction of the body wall muscles. Most homozygous embryos develop normally and hatch into very sluggish first instar larvae. Ca-P60A^Kum170 larvae exposed to 40^oC for 15 minutes show an increased peak of presynaptic Ca²⁺ levels at the neuromuscular junction in response to a 4 minute burst of 30Hz nerve stimulation compared to wild-type animals and a rapid return to baseline levels of Ca²⁺ after cessation of stimulation (in contrast to wild-type animals where the levels of presynaptic Ca²⁺ remain significantly elevated 2 minutes after the cessation of stimulation). Third instar Ca-P60A^Kum170 larvae exposed to 40^oC show a significant reduction in evoked but not spontaneous release at the neuromuscular junction. Spontaneous activity in the dorsal longitudinal muscles (DLMs) of Ca-P60A^Kum170 adults at the permissive temperature is similar to that seen in wild-type adults. However, after a 5 minute exposure to 40^oC, Ca-P60A^Kum170 DLMs show characteristic broad multipeaked action potentials which progress eventually to spike failure (this phenotype is not seen in wild-type adults exposed to 40^oC). Ca-P60A^Kum170 DLM fibres that have not been exposed to 40^oC show frequent action potential failures at a stimulation frequency of 30Hz (in contrast to wild-type fibres which do not show failures in response to stimulation). Ca-P60A^Kum170 DLM fibres that have been exposed to 40^oC show failures at a stimulation frequency of 10Hz. At a given frequency of stimulation, failures are seen earlier in heated Ca-P60A^Kum170 animals than in unheated Ca-P60A^Kum170 animals. Voltage gated calcium currents in muscles are dramatically and chronically reduced in heated Ca-P60A^Kum170 animals, while unheated Ca-P60A^Kum170 animals have calcium currents that are comparable to wild type.

(Sanyal et al., 2005)

When Ca-P60A^Kum170 mutants are exposed to 40⁰C for 4 minutes they show paralysis for up to 48 hours. During this time, the flies show uncoordinated twitches, muscle contraction, and infrequent but intense bouts of seizure-like behavior followed by a return to paralysis. At 20⁰C, Ca-P60A^Kum170 mutants show wild-type levels of DLM activity. However, after 4 minutes of exposure to non-permissive temperatures, DLM of these mutants show infrequent bursts of spontaneous activity.

(Hoeffer et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

SERCA^Kum170 has abnormal heat stress response | dominant phenotype, suppressible by RyR¹⁶/RyR[+]

(Abraham and Wolf, 2013)

SERCA^Kum170, comt⁶ has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^futsch-C380/Sh^{EKO.UAS.GFP(GL)}

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neurophysiology | larval stage phenotype, suppressible by Scer\GAL4^futsch-C380/Sh^{EKO.UAS.GFP(GL)}

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^unspecified/Ras85D^N17.UAS

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neurophysiology | larval stage phenotype, suppressible by Scer\GAL4^unspecified/Ras85D^N17.UAS

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^unspecified/Raf^DN.UAS.cUa

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neurophysiology | larval stage phenotype, suppressible by Scer\GAL4^unspecified/Raf^DN.UAS.cUa

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^unspecified/kay^Fbz.UAS

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neurophysiology | larval stage phenotype, suppressible by Scer\GAL4^unspecified/kay^Fbz.UAS

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neuroanatomy | larval stage phenotype, suppressible by CrebB^UAS.b.cYa/Scer\GAL4^unspecified

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal neurophysiology | larval stage phenotype, suppressible by CrebB^UAS.b.cYa/Scer\GAL4^unspecified

(Freeman et al., 2010)

NOT suppressed by

Statement

Reference

Itpr^wc361/Itpr^ug3, Orai[+]/Orai^k11505, SERCA[+]/SERCA^Kum170 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3, Orai^k11505, SERCA^Kum170 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

SERCA^Kum170 has paralytic | dominant phenotype, non-suppressible by RyR¹⁶/RyR[+]

(Abraham and Wolf, 2013)

Suppressor of

Statement

Reference

SERCA[+]/SERCA^Kum170 is a suppressor | partially of abnormal flight phenotype of Itpr^wc703/Itpr^ug3, NUCB1^c01508, Orai^k11505

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170 is a suppressor | partially of abnormal flight phenotype of Itpr^wc361/Itpr^ug3, NUCB1^c01508, Orai^k11505

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170 is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], NUCB1^c01508, SERCA^Kum170, NUCB1[+] is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508 is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], Orai[+], Orai^k11505, SERCA^Kum170 is a suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170 is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508 is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], NUCB1^c01508, SERCA^Kum170, NUCB1[+] is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], Orai[+], Orai^k11505, SERCA^Kum170 is a suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of flightless | partially phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of flightless | partially phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of abnormal neurophysiology phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of abnormal neurophysiology phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of lethal | cold sensitive phenotype of Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of abnormal neurophysiology phenotype of Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of flightless | partially phenotype of Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2006)

NOT Suppressor of

Statement

Reference

SERCA[+], NUCB1^c01508, SERCA^Kum170, NUCB1[+] is a non-suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508 is a non-suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], NUCB1^NIG.32190R, Scer\GAL4^Ddc.PL, SERCA^Kum170 is a non-suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170 is a non-suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], NUCB1^c01508, SERCA^Kum170, NUCB1[+] is a non-suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170, NUCB1^c01508/NUCB1^c01508 is a non-suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], NUCB1^NIG.32190R, Scer\GAL4^Ddc.PL, SERCA^Kum170 is a non-suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+]/SERCA^Kum170 is a non-suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Other

Statement

Reference

Itpr^wc361/Itpr^ug3, SERCA[+]/SERCA^Kum170 has viable phenotype

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3, SERCA^Kum170 has viable phenotype

(Balasubramanian and Srinivasan, 2020)

SERCA^Kum170, comt⁶ has abnormal neuroanatomy | larval stage phenotype

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has abnormal locomotor behavior | adult stage phenotype

(Freeman et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

SERCA^Kum170 has adult heart phenotype, suppressible by RyR¹⁶/RyR[+]

(Abraham and Wolf, 2013)

SERCA^Kum170, comt⁶ has synapse phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/NFAT^EP1508

(Freeman et al., 2011)

SERCA^Kum170, comt⁶ has synapse phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/NFAT^EP19579

(Freeman et al., 2011)

SERCA^Kum170, comt⁶ has neuromuscular junction | larval stage phenotype, suppressible by Scer\GAL4^futsch-C380/Sh^{EKO.UAS.GFP(GL)}

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has neuromuscular junction | larval stage phenotype, suppressible by Scer\GAL4^unspecified/Ras85D^N17.UAS

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has neuromuscular junction | larval stage phenotype, suppressible by Scer\GAL4^unspecified/Raf^DN.UAS.cUa

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has neuromuscular junction | larval stage phenotype, suppressible by Scer\GAL4^unspecified/kay^Fbz.UAS

(Freeman et al., 2010)

SERCA^Kum170, comt⁶ has neuromuscular junction | larval stage phenotype, suppressible by CrebB^UAS.b.cYa/Scer\GAL4^unspecified

(Freeman et al., 2010)

NOT Enhancer of

Statement

Reference

SERCA^Kum170 is a non-enhancer of dorsal longitudinal indirect flight muscle cell phenotype of comt⁶

(Hoeffer et al., 2003)

Suppressor of

Statement

Reference

Ca-P60A[+]/SERCA^Kum170 is a suppressor of wing phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of wing phenotype of Gα49B[+]/Gαq^221c, Itpr^wc703/Itpr^wc361, Plc21C^k31911/Plc21C[+]

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of wing phenotype of Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2006)

Ca-P60A[+]/SERCA^Kum170 is a suppressor of dorsal longitudinal indirect flight muscle cell phenotype of Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2006)

Other

Statement

Reference

SERCA^Kum170, comt⁶ has synapse phenotype

(Freeman et al., 2011)

SERCA^Kum170, comt⁶ has NMJ bouton | larval stage phenotype

(Freeman et al., 2010)

SERCA^Kum170, comt⁶, para^ts1 has dorsal longitudinal indirect flight muscle cell | heat sensitive phenotype

(Hoeffer et al., 2003)

SERCA^Kum170, comt⁶, para^ts1 has dorsal longitudinal indirect flight muscle cell phenotype

(Hoeffer et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

SERCA^Kum170, Orai^k11505 double heterozygotes do not show flight defects.

(Balasubramanian and Srinivasan, 2020)

RyR¹⁶ partially suppresses the phenotypes seen in heterozygous Ca-P60A^Kum170 mutant flies in response to heat shock. The paralysis is still present but the increase in heart rate and defect in cardiac function are both suppressed. End diastolic dimensions and fractional shortening are also unchanged compared to Ca-P60A^Kum170 alone.

Expression of two copies of Ca-P60A^wt.tinC significantly rescues the cardiac function phenotype seen in wupA^hdp-2 mutant flies. Heart rate is still reduced.

(Abraham and Wolf, 2013)

comt⁶ Ca-P60A^Kum170 double mutants exhibit increased synapse growth and transmitter release.

Scer\GAL4^OK6-driven motor neuron-limited expression of NFAT^EP19579 or NFAT^EP1508 completely abolishes increased synapse growth and transmitter release observed in comt⁶ Ca-P60A^Kum170 double mutants.

(Freeman et al., 2011)

comt⁶ Ca-P60A^Kum170 double mutant flies show increased locomotor activity compared to wild-type controls.

The average number of synaptic boutons at the muscle 6/7 neuromuscular junction is increased in comt⁶ Ca-P60A^Kum170 larvae compared to controls. In addition, synapse strength (neurotransmitter release per action potential) is increased compared to wild type, as is seen in each single mutant.

Expression of Sh^{EKO.Scer\UAS.T:Avic\GFP-GL} under the control of Scer\GAL4^futsch-C380 suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in comt⁶ Ca-P60A^Kum170 larvae.

Expression of either Ras85D^N17.Scer\UAS or phl^{DN.Scer\UAS.cUa} in motor neurons under the control of Scer\GAL4^unspecified suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in comt⁶ Ca-P60A^Kum170 larvae.

Expression of either kay^Fbz.Scer\UAS or CrebB-17A^{Scer\UAS.b.cYa} in motor neurons under the control of Scer\GAL4^unspecified suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in comt⁶ Ca-P60A^Kum170 larvae.

(Freeman et al., 2010)

Larval neurons heterozygous for Ca-P60A^Kum170 in a Itp-r83A^ka1091/Itp-r83A^ug3 background exhibit elevated levels of basal cytosolic Ca[2+].

Ca-P60A^Kum170/olf186-F^EY01467; Itp-r83A^ka1091/Itp-r83A^ug3 flies exhibit normal wings and normal levels of spontaneous electrical activity in DLM recordings. Flight ability is restored in a significant number of these triple mutant flies. Ca-P60A^Kum170/+; Itp-r83A^ka1091/Itp-r83A^ug3 mutants. Over 60% of olf186-F^k11505/Ca-P60A^Kum170; Itp-r83A^ka1091/Itp-r83A^ug3 adults and approximately 50% of olf186-F^EY01467/Ca-P60A^Kum170; Itp-r83A^ka1091/Itp-r83A^ug3 adults pass as 'fliers' in the cylinder drop test assay. Air-puff delivery elicited sustainable rhythmic flight patterns similar to wild-type in a high proportion of these flies.

The presence of Ca-P60A^Kum170 has no effect on Ca[2+] release through the InsP[[3]]R on carbachol stimulation in Itp-r83A^ka1091/Itp-r83A^ug3 mutants.

The presence of olf186-F^EY01467 in olf186-F^EY01467/Ca-P60A^Kum170; Itp-r83A^ka1091/Itp-r83A^ug3 organisms restored carbachol-stimulated Ca[2+] release to wild-type levels.

The presence of a single copy of Ca-P60A^Kum170 delays Ca[2+] sequestration after carbachol-stimulated release, and leads to greater perdurance of the Ca[2+] peak; this effect of Ca-P60A^Kum170 is also present in cells derived from olf186-F^EY01467/Ca-P60A^Kum170;Itp-r83A^ka1091/Itp-r83A^ug3 organisms.

Ca-P60A^Kum170 has a dominant effect and reduces levels of store Ca[2+] in all genotypes tested, including Ca-P60A^Kum170/+; Itp-r83A^ka1091/Itp-r83A^ug3 and Ca-P60A^Kum170/olf186-F^EY01467; Itp-r83A^ka1091/Itp-r83A^ug3. Concurrent with the lower store, SOCE is greatly elevated in Ca-P60A^Kum170 heterozygotes. Significantly , SOCE is normal in neurons derived from Ca-P60A^Kum170/olf186-F^EY01467; Itp-r83A^ka1091/Itp-r83A^ug3, as compared with single and double mutants.

(Venkiteswaran and Hasan, 2009)

The abnormal wing posture phenotype of Itp-r83A^ka1091/Itp-r83A^ug3 animals raised at 25'C is suppressed by Ca-P60A^Kum170/+. Lethality due to Itp-r83A^ka1091/Itp-r83A^ug3 in animals raised at 17'C is also suppressed by Ca-P60A^Kum170/+. The resulting animals have the same abnormal wing posture phenotype as Itp-r83A^ka1091/Itp-r83A^ug3 animals raised at 25'C.

Itp-r83A^ka1091/Itp-r83A^ug3 does not suppress the slow return to basal [Ca[2+]]i levels seen in neurons cultured from Ca-P60A^Kum170/+ larvae following depolarisation with 70mM KCl.

The reduced viability, abnormal wing posture, flight behaviour defects and increased spontaneous firing at NMJs seen in Itp-r83A^wc703/Itp-r83A^wc361; Gα49B^221c/+ animals, and more severely in Itp-r83A^wc703/Itp-r83A^wc361; Gα49B^221c/Plc21C^k31911 animals, are largely suppressed by Ca-P60A^Kum170/+.

(Banerjee et al., 2006)

comt⁶; Ca-P60A^Kum170 double mutants show an increase in strong, spontaneous DLM activity in response to restrictive temperatures that is similar to comt⁶ single mutants. comt⁶ para^ts1; Ca-P60A^Kum170 triple mutants do not show spontaneous DLM firing activity at 33⁰C like comt⁶ and Ca-P60A^Kum170 single mutants, but these triple mutants do show an increase of activity when shifted to 40⁰C, the restrictive temperature for para^ts1 mutants.

(Hoeffer et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

SERCA^KumP52

(Sanyal et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26700

SERCA^Kum170/CyO

Notes on Origin

Discoverer

Selected as: a dominant mutation that results in paralysis of adults within 3 minutes at 40^oC.

(Sanyal et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

170

(Sanyal et al., 2006)

Ca-P60A^Kum170

(Freeman et al., 2011, Banerjee et al., 2006)

Ca-P60A^Kum170ts

(Venkiteswaran and Hasan, 2009)

Ca-P60A^Kum<up>170</up>

(Freeman et al., 2010)

CaP60A^Kum170

(Sanyal et al., 2006, Sanyal et al., 2005)

CaP60A^kum170

(Abraham and Wolf, 2013)

Kum¹⁷⁰

(Balasubramanian and Srinivasan, 2020, Wong et al., 2014, Venkiteswaran and Hasan, 2009, Banerjee et al., 2006, Sanyal et al., 2005)

SERCA^Kum170

SERCA^kum170

(Suisse and Treisman, 2019)

dSERCA^Kum170

(Bi et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (14)

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