FlyBase Allele Report: Dmel\mira[αTub67C.mGFP6]

General Information

Symbol

Dmel\mira^{αTub67C.mGFP6}

Species

D. melanogaster

Name

FlyBase ID

FBal0197531

Feature type

allele

Associated gene

Dmel\mira

Associated Insertion(s)

Carried in Construct

P{tub-mira.GFP}

Also Known As

mira-GFP

Key Links

Transgenic product class

wild_type

(Irion et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Irion et al., 2006)

Mutagen

in vitro construct

(Irion et al., 2006)

Progenitor genotype

Carried in construct

P{tub-mira.GFP}

Cytology

Description

An αTub67C promoter drives expression of the entire mira coding region, tagged with mGFP6.

(Irion et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

αTub67C

Encoded product / tool

mira

Tagged with

mGFP6

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | homeotic

(Irion et al., 2006)

Phenotype Manifest In

embryonic abdomen | ectopic

(Irion et al., 2006)

embryonic head

(Irion et al., 2006)

embryonic thorax

(Irion et al., 2006)

primordial germ cell | ectopic

(Irion et al., 2006)

Detailed Description

Statement

Reference

Expression of mira^{αTub67C.T:Avic\GFP-m6} results in embryos that have an anterior abdomen in place of the head and thorax with 20-75% penetrance. These embryos also have ectopic anterior pole cells.

(Irion et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mira^{αTub67C.mGFP6} has visible | homeotic phenotype, suppressible by stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau²

(Irion et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^1J3-3/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^2C2-3/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^2A2-4/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^2C3-1/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^3C2-2/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^3F1-1/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^x3I2-1/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^2F2-2/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^2B2-8/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot³/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)2[+]/Su(Mir)2^1C2-2

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)2[+]/Su(Mir)2^2H3-1

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)2[+]/Su(Mir)2^1E3-5

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)2^1J3-4b/Su(Mir)2[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)3^1H2-1a/Su(Mir)3[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)3[+]/Su(Mir)3^1A2-1

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)3^1G3-1a/Su(Mir)3[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by capu[+]/capu^3G3-1

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible | partially by Df(2L)ed1/+

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by capu^EY12344/capu[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible | partially by spir[+]/spir¹

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible | partially by spir[+]/spir^2F

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)1-1A2-2[+]/Su(Mir)1-1A2-2^1A2-2

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)1E2-4^1E2-4/Su(Mir)1E2-4[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)1A4-1[+]/Su(Mir)1A4-1^1A4-1

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)3D1-1[+]/Su(Mir)3D1-1^3D1-1

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)2A2-1[+]/Su(Mir)2A2-1^2A2-1

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)3C2-1^3C2-1/Su(Mir)3C2-1[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)2E2-3[+]/Su(Mir)2E2-3^2E2-3

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)1E3-2^1E3-2/Su(Mir)1E3-2[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)2C2-1^2C2-1/Su(Mir)2C2-1[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)1F2-3[+]/Su(Mir)1F2-3^1F2-3

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by Su(Mir)3-1A2-2[+]/Su(Mir)3-1A2-2^1A2-2

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by osk[+]/osk^A87

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot^1E2-5/shot[+]

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by shot[+]/shot^1J3-2

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic abdomen | ectopic phenotype, suppressible by stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau²

(Irion et al., 2006)

mira^{αTub67C.mGFP6} has embryonic head phenotype, suppressible by stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau²

(Irion et al., 2006)

mira^{αTub67C.mGFP6} has embryonic thorax phenotype, suppressible by stau^{ΔdRBD5.αTub67C.Tag:MYC}/stau²

(Irion et al., 2006)

NOT suppressed by

Statement

Reference

mira^{αTub67C.mGFP6} has embryonic head phenotype, non-suppressible by capu[+]/capu²

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, non-suppressible by capu^UASp.cDa.GFP/Scer\GAL4^{VP16.nanos.UTR}

(Chang et al., 2011)

mira^{αTub67C.mGFP6} has embryonic head phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP

(Chang et al., 2011)

Other

Statement

Reference

mira^{αTub67C.mGFP6}, stau^{ΔdRBD5.αTub67C.Tag:MYC}, stau² has embryonic abdomen phenotype

(Irion et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is almost completely suppressed (99.2%) if the females are also heterozygous for osk^A87. Although these embryos form normal heads, only 0.6% of them hatch.

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is suppressed if the females are also heterozygous for one of shot^1E2-5, shot^1J3-2, shot^1J3-3, shot^2C2-3, shot^2A2-4, shot^2C3-1, shot^3C2-2, shot^3F1-1, shot^x3I2-1, shot^2F2-2, shot^2B2-8, shot³, Su(Mir)2^1C2-2, Su(Mir)2^2H3-1, Su(Mir)2^1E3-5, Su(Mir)2^1J3-4b, Su(Mir)3^1H2-1a, Su(Mir)3^1A2-1, Su(Mir)3^1G3-1a, capu^3G3-1, Df(2L)ed1, capu^EY12344, spir¹, spir^2F, Su(Mir)1-1A2-2^1A2-2, Su(Mir)1E2-4^1E2-4, Su(Mir)1A4-1^1A4-1, Su(Mir)3D1-1^3D1-1, Su(Mir)2A2-1^2A2-1, Su(Mir)3C2-1^3C2-1, Su(Mir)2E2-3^2E2-3, Su(Mir)1E3-2^1E3-2, Su(Mir)2C2-1^2C2-1, Su(Mir)1F2-3^1F2-3 or Su(Mir)3-1A2-2^1A2-2.

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is not suppressed if the females are also heterozygous for capu².

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is not suppressed if the females are also expressing either capu^{Scer\UAS.P\T.cDa.T:Avic\GFP} or capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Chang et al., 2011)

Embryos that express both mira^{αTub67C.T:Avic\GFP-m6} and stau^{ΔdRBD5.αTub67C.T:Hsap\MYC} in a stau² background show the stau^{ΔdRBD5.αTub67C.T:Hsap\MYC} posterior group phenotype in which embryos develop a normal head and thorax, but lack the abdomen.

(Irion et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mira^{αTub67C.T:Avic\GFP-m6}

mira^{αTub67C.mGFP6}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close