FlyBase Allele Report: Dmel\Dref[RNAi.h-h.UAS]

General Information

Symbol

Dmel\Dref^RNAi.h-h.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0197919

Feature type

allele

Associated gene

Dmel\Dref

Associated Insertion(s)

Carried in Construct

P{UAS-Dref.RNAi.h-h}

Also Known As

UAS-DREF-RNAi, UAS-dref-IR

Key Links

Transgenic product class

RNAi_reagent

(Yoshida et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yoshida et al., 2004)

Mutagen

in vitro construct

(Yoshida et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Dref.RNAi.h-h}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of Dref sequences (1kb and 750bp of N-terminal sequences), in head-to-head orientation.

(Yoshida et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dref

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

lethal, with Scer\GAL4³⁷³⁵

(Yoshida et al., 2004)

lethal, with Scer\GAL4^ap-md544

(Kawamori and Yamaguchi, 2011)

lethal, with Scer\GAL4^dpp.blk1

(Yoshida et al., 2004)

lethal, with Scer\GAL4^en-e16E

(Yoshida et al., 2004)

lethal | larval stage, with Scer\GAL4^T80

(Yoshida et al., 2004)

lethal | pupal stage, with Scer\GAL4^T80

(Yoshida et al., 2004)

lethal - all die before end of larval stage, with Scer\GAL4^32B

(Yoshida et al., 2004)

lethal - all die before end of larval stage, with Scer\GAL4^Act5C.PHb

(Yoshida et al., 2004)

lethal - all die before end of larval stage, with Scer\GAL4^Cg.PA

(Yoshida et al., 2004)

lethal - all die before end of pupal stage, with Scer\GAL4^Act5C.PI

(Yoshida et al., 2004)

melanotic mass phenotype | larval stage, with Scer\GAL4^Cg.PA

(Yoshida et al., 2004)

some die during larval stage, with Scer\GAL4^32B

(Yoshida et al., 2004)

some die during larval stage, with Scer\GAL4^Act5C.PHb

(Yoshida et al., 2004)

some die during larval stage, with Scer\GAL4^Cg.PA

(Yoshida et al., 2004)

some die during pupal stage, with Scer\GAL4^Act5C.PI

(Yoshida et al., 2004)

visible, with Scer\GAL4^en-e16E

(Yoshida et al., 2004)

visible, with Scer\GAL4^GMR.PS

(Yoshida et al., 2004)

Phenotype Manifest In

anterior dorsocentral bristle, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

anterior scutellar bristle, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

external sensory organ precursor cell, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

eye, with Scer\GAL4^GMR.PS

(Yoshida et al., 2004)

macrochaeta, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

melanotic mass, with Scer\GAL4^Cg.PA

(Yoshida et al., 2004)

microchaeta, with Scer\GAL4^ap-md544

(Kawamori and Yamaguchi, 2011)

nucleolus, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

nucleus, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

posterior dorsocentral bristle, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

posterior notopleural bristle, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

posterior scutellar bristle, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

presutural bristle, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

tormogen cell, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

trichogen cell, with Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

wing, with Scer\GAL4^en-e16E

(Yoshida et al., 2004)

wing margin bristle | somatic clone, with Scer\GAL4^Act5C.PI

(Kawamori and Yamaguchi, 2011)

wing vein L5, with Scer\GAL4^en-e16E

(Yoshida et al., 2004)

Detailed Description

Statement

Reference

Expression of one copy of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) using Scer\GAL4^sca-109-68 results in thorax macrochaetes becoming thin and short compared to controls. Bristles in the anterior and posterior scutellar, anterior and posterior dorsocentral, posterior notopleural and presutural areas are most strongly affected.

Expression of two copies of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) using Scer\GAL4^sca-109-68 results in deletion of almost all thorax macrochaetes. However, P{lArB}neur^A101-positive posterior scutellar cells are detected under the notum in about 60% of cases, indicating that bristle cell lineages may still exist.

Expression of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) using Scer\GAL4^sca-109-68 results in death of most flies. Expression of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}10) using Scer\GAL4^sca-109-68 results in almost all thorax microchaetes becoming short and thin.

Expression of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) in clones induced with Scer\GAL4^{Scer\FRT.Act5C} results in the stout mechanosensory bristles of the anterior wing margin becoming short and thin.

Sensory organ precursors of the thoracic bristles are specified normally when Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) is expressed using Scer\GAL4^sca-109-68 in wing discs.

Expression of Dref^{dsRNA.h-h.Scer\UAS} using Scer\GAL4^ac.13 does not affect thoracic bristle development.

In most cases, cell differentiation within the posterior scutellar sensory organ precursor (SOP) lineage occurs correctly when two copies of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) are expressed using Scer\GAL4^sca-109-68. However, the onset of timing of the first and second asymmetric divisions of the SOP are delayed.

The nuclear size of the posterior scutellar (PSC) shaft and socket cell are smaller in flies expressing 2 copies of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) using Scer\GAL4^sca-109-68 than controls: at 30 hours after puparium formation, the shaft cell nucleus is reduced in size by half, while the socket cell nucleus is reduced by 70%. Rates of BrDU incorporation in the PSC shaft cell, and possibly the socket cell, are lower than controls.

Over-expression of two copies of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) using Scer\GAL4^sca-109-68 decreases the nuclear and nucleolar size of the posterior scutellar shaft cells.

(Kawamori and Yamaguchi, 2011)

Expression of Dref^{dsRNA.h-h.Scer\UAS} under the control of Scer\GAL4^GMR.PS results in a rough eye phenotype.

Expression of Dref^{dsRNA.h-h.Scer\UAS} under the control of Scer\GAL4^en-e16E can result in lethality, or can result in a notched wing phenotype, depending on the P{UAS-Dref.IR.h-h} insertion line used. When P{UAS-Dref.IR.h-h}10 is expressed under the control of Scer\GAL4^en-e16E, the wings are notched and there is a loss of wing vein L5 close to the distal margin.

Expression of Dref^{dsRNA.h-h.Scer\UAS} under the control of one of Scer\GAL4^ey.PH, Scer\GAL4³¹, Scer\GAL4^48Y, Scer\GAL4^twi.PG, Scer\GAL4^e22c, Scer\GAL4^prd.RG1, Scer\GAL4^Dll-md23, Scer\GAL4^arm.PS, Scer\GAL4^Hml.PG, Scer\GAL4^bs-1348, Scer\GAL4^OK107, Scer\GAL4^rho.NEE, Scer\GAL4^wg.PM or Scer\GAL4^elav.PLu results in no detectable phenotype in the eye or wing.

Expression of Dref^{dsRNA.h-h.Scer\UAS} under the control of Scer\GAL4^Cg.PA results in melanotic tumours in larvae and the animals die at the larval stage.

(Yoshida et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Dref^RNAi.h-h.UAS, Scer\GAL4^sca-109-68 has posterior scutellar bristle phenotype, enhanceable by Df(2R)173/+

(Kawamori and Yamaguchi, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The thin and short posterior scutellar bristles seen when one copy of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15) is expressed using Scer\GAL4^sca-109-68 become even shorter in a Df(2R)173/+ background (in which the mus209 locus is deleted).

(Kawamori and Yamaguchi, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dref^RNAi.h-h.UAS is rescued by Dref^UAS.cSa/Scer\GAL4^sca-109-68

(Kawamori and Yamaguchi, 2011)

Comments

Co-expression of Dref^Scer\UAS.cSa effectively rescues the bristle phenotypes resulting from Scer\GAL4^sca-109-68-mediated expression of Dref^{dsRNA.h-h.Scer\UAS} (insertion P{UAS-Dref.IR.h-h}15).

(Kawamori and Yamaguchi, 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dref^RNAi.h-h.UAS

Dref^{dsRNA.h-h.Scer\UAS}

Dref^{dsRNA.h-h.UAS}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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