UASt regulatory sequences drive expression of an inverted repeat.
At 25[o]C, expression of ND-39GD5150 driven by Scer\GAL4da.G32 is lethal; at 21[o]C it is not longer lethal.
Expression of ND-39GD5150 driven by Scer\GAL4da.PU leads to developmental delay in male and female flies.
Adults expressing ND-39GD5150 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late larval lethality.
Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.
Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 0% or 10-20% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 40-50% or 70-80% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 20-30% or 60-70% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.
ND-39GD5150, Scer\GAL4da.G32 has lethal | temperature conditional phenotype, suppressible | partially by Cint\AOXUAS.cFa, Scer\GAL4da.G32
ND-39GD5150, Scer\GAL4da.G32 has lethal phenotype, suppressible by Scer\NDI1UAS.cSa, Scer\GAL4da.G32
ND-39GD5150, Scer\GAL4da.PU, Scer\NDI1UAS.cSa has bang sensitive phenotype
Co-expression of Cint\AOXScer\UAS.cFa does not suppress lethality seen with expression of ND-39GD5150 driven by Scer\GAL4da.G32 at 25[o]C; at 21[o]C it significantly increases the number of progeny eclosing.
Flies with expression of ND-39GD5150 and Scer\NDI1Scer\UAS.cSa driven by Scer\GAL4da.PU show significantly increased recovery time (bang sensitivity) compared to controls.
Co-expression of Scer\NDI1Scer\UAS.cSa partially suppresses lethality in flies with expression of ND-39GD5150 driven by Scer\GAL4da.G32.