FlyBase Allele Report: Scer\NDI1[UAS.cSa]

General Information

Symbol

Scer\NDI1^UAS.cSa

Species

S. cerevisiae

Name

Saccharomyces cerevisiae UAS construct a of Sanz

FlyBase ID

FBal0319555

Feature type

allele

Associated gene

Scer\NDI1

Associated Insertion(s)

Carried in Construct

P{UAS-NDI1.S}

Also Known As

UAS-Ndi1

Transgenic product class

wild_type

(Sanz et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sanz et al., 2010)

Mutagen

in vitro construct

(Sanz et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-NDI1.S}

Cytology

Description

UAS regulatory sequences drive expression of the Scer\NDI1 coding region.

(Sanz et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Scer\NDI1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile, with Scer\GAL4^da.G32

(Sanz et al., 2010)

long lived, with Scer\GAL4^da.G32

(Sanz et al., 2010)

long lived | calorie restriction conditional, with Scer\GAL4^da.G32

(Sanz et al., 2010)

long lived | chemical conditional, with Scer\GAL4^da.G32

(Sanz et al., 2010)

long lived | nutrition conditional, with Scer\GAL4^da.G32

(Sanz et al., 2010)

viable, with Scer\GAL4^da.G32

(Sanz et al., 2010)

Phenotype Manifest In

mitochondrion | adult stage | progressive, with Scer\GAL4^da.G32

(Sanz et al., 2010)

Detailed Description

Statement

Reference

No dopaminergic neuron loss is observed when Scer\NDI1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^ple.PF.

(Humphrey et al., 2012)

Flies with expression of Scer\NDI1^Scer\UAS.cSa driven by Scer\GAL4^da.G32 are viable and fertile, with no morphological, behavioral or reproductive abnormalities. Scer\GAL4^da.G32>Scer\NDI1^Scer\UAS.cSa flies show significant resistance to rotenone or paraquat but not antimycin, compared to controls; the range of drug concentration where resistance is seen is narrow and males appear more resistant than females. Scer\GAL4^da.G32>Scer\NDI1^Scer\UAS.cSa flies have a significantly longer lifespan than controls; this is more pronounced in males than females. Scer\GAL4^da.G32>Scer\NDI1^Scer\UAS.cSa flies have significantly longer lifespans than controls when raised on rich medium or under dietary restriction conditions. Isolated mitochondria from aged Scer\GAL4^da.G32>Scer\NDI1^Scer\UAS.cSa flies (in contrast to young flies) show increases in substrate oxidation and decreases in mitochondrial ROS production, compared to controls.

(Sanz et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\NDI1^UAS.cSa, Scer\GAL4^elav-C155 is an enhancer of short lived | male phenotype of COX5B^GD6659, Scer\GAL4^elav-C155

(Kemppainen et al., 2014)

Scer\NDI1^UAS.cSa, Scer\GAL4^elav.PLu is an enhancer of short lived phenotype of COX4^KK107510, Scer\GAL4^elav.PLu

(Kemppainen et al., 2014)

Suppressor of

Statement

Reference

Scer\NDI1^UAS.cSa, Scer\GAL4^Act5C.PU is a suppressor | partially of partially lethal - majority die phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Scer\NDI1^UAS.cSa, Scer\GAL4^Act5C.PU is a suppressor | partially of some die during larval stage phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Scer\NDI1^UAS.cSa, Scer\GAL4^Act5C.PU is a suppressor | partially of some die during pupal stage phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Scer\NDI1^UAS.cSa, Scer\GAL4^da.G32 is a suppressor of lethal phenotype of ND-19^GD16787, Scer\GAL4^da.G32

(Sanz et al., 2010)

Scer\NDI1^UAS.cSa, Scer\GAL4^da.G32 is a suppressor of lethal phenotype of ND-39^GD5150, Scer\GAL4^da.G32

(Sanz et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\NDI1^UAS.cSa, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during larval stage phenotype of COX4^KK107510, Scer\GAL4^da.PU

(Kemppainen et al., 2014)

Scer\NDI1^UAS.cSa, Scer\GAL4^da.PU is a non-suppressor of abnormal developmental rate | larval stage phenotype of COX4^KK107510, Scer\GAL4^da.PU

(Kemppainen et al., 2014)

Scer\NDI1^UAS.cSa, Scer\GAL4^elav-C155 is a non-suppressor of partially lethal - majority die phenotype of COX4^KK107510, Scer\GAL4^elav-C155

(Kemppainen et al., 2014)

Scer\NDI1^UAS.cSa, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during P-stage phenotype of COX7A^KK110870, Scer\GAL4^da.PU

(Kemppainen et al., 2014)

Scer\GAL4^da.PU/Scer\NDI1^UAS.cSa is a non-suppressor of bang sensitive phenotype of levy¹

(Kemppainen et al., 2014)

Scer\NDI1^UAS.cSa, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during larval stage phenotype of COX5B^GD6659, Scer\GAL4^da.PU

(Kemppainen et al., 2014)

Scer\NDI1^UAS.cSa, Scer\GAL4^elav-C155 is a non-suppressor of partially lethal - majority die phenotype of COX5B^GD6659, Scer\GAL4^elav-C155

(Kemppainen et al., 2014)

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of abnormal neuroanatomy phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Other

Statement

Reference

Scer\GAL4^da.G32, Scer\NDI1^UAS.cSa, tko[+]/tko^25t has partially lethal phenotype

(Kemppainen et al., 2014)

Scer\GAL4^da.G32, Scer\NDI1^UAS.cSa, tko^25t has partially lethal - majority die phenotype

(Kemppainen et al., 2014)

Cint\AOX^UAS.cFa, Scer\GAL4^da.G32, Scer\NDI1^UAS.cSa, tko[+]/tko^25t has partially lethal phenotype

(Kemppainen et al., 2014)

Cint\AOX^UAS.cFa, Scer\GAL4^da.G32, Scer\NDI1^UAS.cSa, tko^25t has lethal - all die before end of P-stage phenotype

(Kemppainen et al., 2014)

COX5B^GD6659, Scer\GAL4^elav-C155, Scer\NDI1^UAS.cSa has short lived | female phenotype

(Kemppainen et al., 2014)

ND-39^GD5150, Scer\GAL4^da.PU, Scer\NDI1^UAS.cSa has bang sensitive phenotype

(Kemppainen et al., 2014)

ND-19^GD16787, Scer\GAL4^da.PU, Scer\NDI1^UAS.cSa has bang sensitive phenotype

(Kemppainen et al., 2014)

Cint\AOX^UAS.cFa, Scer\GAL4^da.G32, Scer\NDI1^UAS.cSa has partially lethal - majority die phenotype

(Sanz et al., 2010)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of dopaminergic PPL1 neuron phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of dopaminergic PPL2ab neuron phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of dopaminergic PPL2c neuron phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of dopaminergic PPM1 neuron phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of dopaminergic PPM2 neuron phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of dopaminergic PPM3 neuron phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa is a non-suppressor of dopaminergic PAL neuron phenotype of PolG1^KK100485, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Scer\NDI1^Scer\UAS.cSa under the control of Scer\GAL4^da.G32 in tko^25t/tko^25t mutants results in nearly complete lethality, and in tko^25t/+ mutants results in partial lethality.

Co-expression of Zzzz\AOX^Scer\UAS.cFa and Scer\NDI1^Scer\UAS.cSa under the control of Scer\GAL4^da.G32 in tko^25t/tko^25t mutants results in complete lethality, and in tko^25t/+ mutants results in partial lethality.

(Kemppainen et al., 2014)

Co-expression of Scer\NDI1^Scer\UAS.cSa does not rescue lethality in flies in flies with expression of COX4^KK107510, COX5B^GD6659 or COX7A^KK110870 driven by Scer\GAL4^da.PU. Co-expression of Scer\NDI1^Scer\UAS.cSa leads to a shortened lifespan in females and further enhances the shortened lifespan in males with expression of COX5B^GD6659 driven by Scer\GAL4^elav-C155. Co-expression of Scer\NDI1^Scer\UAS.cSa enhances the shortened lifespan in males with expression of COX4^KK107510 driven by Scer\GAL4^elav.PLu. Expression of Scer\NDI1^Scer\UAS.cSa driven by Scer\GAL4^da.PU does not significantly suppress bang sensitivity in levy¹ flies. Flies with expression of ND-39^GD5150 or ND-19^GD16787, along with Scer\NDI1^Scer\UAS.cSa, driven by Scer\GAL4^da.PU show significantly increased recovery time (bang sensitivity) compared to controls.

(Kemppainen et al., 2014)

Co-expression of Zzzz\AOX^Scer\UAS.cFa leads to lethality (less than 2% survivors) in flies with expression of Scer\NDI1^Scer\UAS.cSa driven by Scer\GAL4^da.G32.

Co-expression of Scer\NDI1^Scer\UAS.cSa suppresses lethality in flies with expression of ND-19^GD16787 or ND-39^GD5150 driven by Scer\GAL4^da.G32.

(Sanz et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93878

w¹¹¹⁸; P{UAS-NDI1.S}A46

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\NDI1^Scer\UAS.cSa

Scer\NDI1^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sanz

Secondary FlyBase IDs

References (6)

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