FlyBase Allele Report: Dmel\ND-19[GD16787]

General Information

Symbol

Dmel\ND-19^GD16787

Species

D. melanogaster

Name

FlyBase ID

FBal0204033

Feature type

allele

Associated gene

Dmel\ND-19

Associated Insertion(s)

Carried in Construct

P{GD16787}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16787}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16787

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^da.PU

(Kemppainen et al., 2014)

lethal, with Scer\GAL4^da.G32

(Sanz et al., 2010)

lethal | temperature conditional, with Scer\GAL4^da.G32

(Gospodaryov et al., 2014)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mitochondrion, with Scer\GAL4^ple.PF

(Esposito et al., 2013)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

At 25[o]C, expression of ND-19^GD16787 driven by Scer\GAL4^da.G32 is lethal; at 21[o]C there are a tiny number of escapers.

(Gospodaryov et al., 2014)

Expression of ND-19^GD16787 driven by Scer\GAL4^da.PU leads to developmental delay in male and female flies.

(Kemppainen et al., 2014)

Mitochondrial preparations from animals expressing NDUFA8^GD16787 under the control of Scer\GAL4^da.G32 produce higher levels of O[[2]][-] (superoxide) than wild-type mitochondria.

Expression of NDUFA8^GD16787 under the control of Scer\GAL4^ple.PF in dopaminergic neurons results in mitochondria forming aggregates.

(Esposito et al., 2013)

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 30-40% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16787

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ND-19^GD16787, Scer\GAL4^da.G32 has lethal | temperature conditional phenotype, suppressible | partially by Cint\AOX^UAS.cFa, Scer\GAL4^da.G32

(Gospodaryov et al., 2014)

ND-19^GD16787, Scer\GAL4^da.G32 has lethal phenotype, suppressible by Scer\NDI1^UAS.cSa, Scer\GAL4^da.G32

(Sanz et al., 2010)

Other

Statement

Reference

ND-19^GD16787, Scer\GAL4^da.PU, Scer\NDI1^UAS.cSa has bang sensitive phenotype

(Kemppainen et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

ND-19^GD16787, Scer\GAL4^ple.PF has mitochondrion phenotype, suppressible | partially by mAcon1¹/Acon[+]

(Esposito et al., 2013)

ND-19^GD16787 has mitochondrion phenotype, suppressible by Scer\GAL4^ple.PF/Fer3HCH^UAS.cMa

(Esposito et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Acon¹/+ partially suppresses the defects in mitochondria morphology seen in the dopaminergic neurons of animals NDUFA8^GD16787 under the control of Scer\GAL4^ple.PF.

Co-expression of Fer3HCH^Scer\UAS.cMa partially suppresses the defects in mitochondria morphology seen in the dopaminergic neurons of animals NDUFA8^GD16787 under the control of Scer\GAL4^ple.PF.

(Esposito et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of Cint\AOX^Scer\UAS.cFa does not suppress lethality seen with expression of ND-19^GD16787 driven by Scer\GAL4^da.G32 at 25[o]C; at 21[o]C it significantly increases the number of progeny eclosing.

(Gospodaryov et al., 2014)

Flies with expression of ND-19^GD16787 and Scer\NDI1^Scer\UAS.cSa driven by Scer\GAL4^da.PU show significantly increased recovery time (bang sensitivity) compared to controls.

(Kemppainen et al., 2014)

Co-expression of Scer\NDI1^Scer\UAS.cSa partially suppresses lethality in flies with expression of ND-19^GD16787 driven by Scer\GAL4^da.G32.

(Sanz et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v46799

w¹¹¹⁸; P{GD16787}v46799/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16787

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG3683^GD16787

ND-19^GD16787

NDUFA8^GD16787

NDUFA8^RNAi

(Esposito et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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