FlyBase Allele Report: Dmel\PolG1[KK100485]

Expression of tam^KK100485 under the control of Scer\GAL4^{bam.T:Hsim\VP16} prevents the elimination of mitochondrial nucleoids (containing the mtDNA) in developing spermatids.

(Yu et al., 2017)

Expression of tam^KK100485 under the control of Scer\GAL4^Act5C.PU results in reduced complex I activity and ATP levels compared with controls. Flies have a significantly shortened lifespan compared to controls.

Flies expressing tam^KK100485 under the control of Scer\GAL4^tub.PU (limited to the adult stages using Scer\GAL80^ts.αTub84B) have a significantly shortened lifespan compared to controls.

Flies expressing tam^KK100485 in cholinergic neurons under the control of Scer\GAL4^Cha.7.4 (co-expressed with Dcr-2^Scer\UAS.cDa) show an age-related decline in climbing performance similar to controls. Flies do not exhibit any differences in spontaneous activity, speed of walking or total distance traveled. No neuron loss is seen in the brain of these flies.

Flies expressing tam^KK100485 in dopaminergic neurons under the control of Scer\GAL4^ple.PF (co-expressed with Dcr-2^Scer\UAS.cDa) show a significant and progressive age-related decline in climbing performance when compared with controls. These flies have a normal lifespan but show decreased activity and, when moving, they walk at a slower speed and cover less distance compared with controls. Expression under the control of Scer\GAL4^ple.PF results in a significant and age-related decrease in dopaminergic neurons. PPL1 and PPM1/2 dopaminergic neuron clusters show progressive loss of neurons from 31 to 70 days, whereas PPM3 and PAL clusters show significant early loss( at 31 days) but no significant difference is seen with age.

When compared with age-matched controls, flies expressing tam^KK100485 in serotonergic neurons under the control of Scer\GAL4^Trh.PB (co-expressed with Dcr-2^Scer\UAS.cDa) do not show decreased climbing performance. A small but significantly significant reduction in activity is seen in aged flies. Serotonergic neuron loss is seen only in aged flies. No neuron loss is seen at 5 days, but significant loss is present by 31 days.

A significant loss of dopaminergic neurons, but not serotonergic neurons, is seen in 60 day old flies expressing tam^KK100485 under the control of Scer\GAL4^elav.PU (co-expressed with Dcr-2^Scer\UAS.cDa). Cell loss affects all dopaminergic clusters except PAL.

(Humphrey et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PolG1^KK100485, Scer\GAL4^Act5C.PU has partially lethal - majority die phenotype, suppressible | partially by park^UAS.cHa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during larval stage phenotype, suppressible | partially by park^UAS.cHa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during pupal stage phenotype, suppressible | partially by park^UAS.cHa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has partially lethal - majority die phenotype, suppressible | partially by Drp1^UAS.cDa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during larval stage phenotype, suppressible | partially by Drp1^UAS.cDa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during pupal stage phenotype, suppressible | partially by Drp1^UAS.cDa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has partially lethal - majority die phenotype, suppressible | partially by dhd^UAS.cUa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during larval stage phenotype, suppressible | partially by dhd^UAS.cUa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during pupal stage phenotype, suppressible | partially by dhd^UAS.cUa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has partially lethal - majority die phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during larval stage phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during pupal stage phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has abnormal neuroanatomy phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has partially lethal - majority die phenotype, suppressible | partially by Scer\NDI1^UAS.cSa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during larval stage phenotype, suppressible | partially by Scer\NDI1^UAS.cSa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during pupal stage phenotype, suppressible | partially by Scer\NDI1^UAS.cSa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

NOT suppressed by

Statement

Reference

PolG1^KK100485, Scer\GAL4^da.PU has increased mortality during development phenotype, non-suppressible by Cint\AOX^mut.UAS, Scer\GAL4^da.PU

(Rodrigues et al., 2018)

PolG1^KK100485, Scer\GAL4^da.PU has increased mortality during development phenotype, non-suppressible by Cint\AOX^wt.UAS, Scer\GAL4^da.PU

(Rodrigues et al., 2018)

PolG1^KK100485, Scer\GAL4^da.PU has increased mortality during development phenotype, non-suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^da.PU

(Rodrigues et al., 2018)

PolG1^KK100485, Scer\GAL4^Act5C.PU has partially lethal - majority die phenotype, non-suppressible by ple^UAS.cFa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during larval stage phenotype, non-suppressible by ple^UAS.cFa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^Act5C.PU has some die during pupal stage phenotype, non-suppressible by ple^UAS.cFa, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPL1 neuron phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPL2ab neuron phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPL2c neuron phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPM1 neuron phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPM2 neuron phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPM3 neuron phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PAL neuron phenotype, suppressible by Cint\AOX^UAS.cFa, Scer\GAL4^ple.PF

(Humphrey et al., 2012)

NOT suppressed by

Statement

Reference

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPL1 neuron phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPL2ab neuron phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPL2c neuron phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPM1 neuron phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPM2 neuron phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PPM3 neuron phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

PolG1^KK100485, Scer\GAL4^ple.PF has dopaminergic PAL neuron phenotype, non-suppressible by Scer\GAL4^ple.PF/Scer\NDI1^UAS.cSa

(Humphrey et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of park^Scer\UAS.cHa partially suppresses the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU.

Expression of Drp1^Scer\UAS.cDa partially suppresses the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU.

Expression of dhd^Scer\UAS.cUa partially suppresses the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU.

Expression of ple^Scer\UAS.cFa does not suppress the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU.

(Humphrey et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Scer\NDI1^Scer\UAS.cSa partially suppresses the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU.

Expression of Zzzz\AOX^Scer\UAS.cFa completely suppresses the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU. The reduction in total ATP content is also suppressed.

Expression of Zzzz\AOX^Scer\UAS.cFa completely suppresses the dopaminergic neuron loss seen when tam^KK100485 is expressed under the control of Scer\GAL4^ple.PF.

(Humphrey et al., 2012)

Complementation and Rescue Data

Rescued by

PolG1^KK100485 is rescued by PolG1^{UASp.RR.Tag:FLAG}/Scer\GAL4^VP16.bam

(Yu et al., 2017)

PolG1^KK100485 is rescued by PolG1^{D263A.UASp.RR.Tag:FLAG}/Scer\GAL4^VP16.bam

(Yu et al., 2017)

Partially rescued by

PolG1^KK100485 is partially rescued by PolG1^{E813V.UASp.RR.Tag:FLAG}/Scer\GAL4^VP16.bam

(Yu et al., 2017)

Comments

The failure to eliminate mitochondrial nucleoid and mtDNA during spermatid development characteristic for males expressing tam^KK100485 under the control of Scer\GAL4^{bam.T:Hsim\VP16} can be fully rescued by co-expression of either tam^{UASp.RR.Tag:FLAG} or tam^{D263A.UASp.RR.Tag:FLAG} and is partially rescued by co-expression of tam^{E813V.UASp.RR.Tag:FLAG} but the spermatids still retain some nucleoids during late individualization stage.

(Yu et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106955

P{KK100485}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PolG1^KK100485

tam^KK100485

Name Synonyms

Secondary FlyBase IDs

References (6)

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