FlyBase Allele Report: Dmel\ple[UAS.cFa]

General Information

Symbol

Dmel\ple^UAS.cFa

Species

D. melanogaster

Name

FlyBase ID

FBal0249083

Feature type

allele

Associated gene

Dmel\ple

Associated Insertion(s)

Carried in Construct

P{UAS-DTHg}

Also Known As

UAS-DTHg, DTHg

Key Links

Transgenic product class

genomic_DNA

(Cichewicz et al., 2017)

wild_type

(Cichewicz et al., 2017, Friggi-Grelin et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Cichewicz et al., 2017)

wild_type

(Cichewicz et al., 2017, Friggi-Grelin et al., 2003)

Mutagen

in vitro construct

(Friggi-Grelin et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-DTHg}

Cytology

Description

In the absence of a GAL4 driver, the P{UAS-DTHg} construct shows complete rescue of flies that lack ple function in the central nervous system. This is most likely due to expression driven by intragenic transcriptional regulatory elements (the ple sequence in this construct is a genomic fragment including introns).

(Cichewicz et al., 2017)

UASt regulatory sequences drive expression of the whole transcribed region of ple.

(Friggi-Grelin et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

ple

UASt

binary expression system - regulatory region

Encoded product / tool

ple

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal phototaxis, with Scer\GAL4^Ddc.PL, Scer\GAL4^ple.PF, ple²

(Riemensperger et al., 2011)

abnormal visual behavior, with Scer\GAL4^Ddc.PL, Scer\GAL4^ple.PF, ple²

(Riemensperger et al., 2011)

chemical resistant, with Scer\GAL4^ple.PF

(Cassar et al., 2015)

chemical resistant, with Scer\GAL4^Trhn.PB

(Cassar et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of ple^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF results in increased resistance of flies to paraquat intoxication. Increased survival rates are seen at 24 hours after ingestion and 2 hours after direct application to the ventral nerve cord (VNC) of decapitated flies.

Expression of ple^Scer\UAS.cFa in serotonergic neurons under the control of Scer\GAL4^Trh.PB results in increased resistance of flies to paraquat intoxication. Increased survival rates are seen at 24 hours after ingestion and 2 hours after direct application to the ventral nerve cord (VNC) of decapitated flies.

(Cassar et al., 2015)

Flies expressing ple^Scer\UAS.cFa in a ple² background (under the control of both Scer\GAL4^ple.PF and Scer\GAL4^Ddc.PL) display slightly less accurate visual orientation than wild-type. Similarly, using the detour paradigm, both ple^Scer\UAS.cFa; ple² and ple^FS.Scer\UAS; ple² flies demonstrate a roughly wild-type spatial orientation memory.

ple² flies expressing either ple^Scer\UAS.cFa under the control of both Scer\GAL4^ple.PF and Scer\GAL4^Ddc.PL exhibit normal adult life expectancy.

Flies expressing ple^Scer\UAS.cFa in a ple² background (under the control of both Scer\GAL4^ple.PF and Scer\GAL4^Ddc.PL) demonstrate associative memory similar to wild-type, indicating normal olfactory learning.

Flies expressing ple^Scer\UAS.cFa in a ple² background (under the control of both Scer\GAL4^ple.PF and Scer\GAL4^Ddc.PL) demonstrate partial rescue of ple² phototactic behavior.

(Riemensperger et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ple^UAS.cFa, Scer\GAL4^ple.TH-D is a suppressor of abnormal flight phenotype of Itpr^NIG.1063R, Scer\GAL4^ple.TH-D

(Pathak et al., 2015)

NOT Suppressor of

Statement

Reference

ple^UAS.cFa, Scer\GAL4^Act5C.PU is a non-suppressor of partially lethal - majority die phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

ple^UAS.cFa, Scer\GAL4^Act5C.PU is a non-suppressor of some die during larval stage phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

ple^UAS.cFa, Scer\GAL4^Act5C.PU is a non-suppressor of some die during pupal stage phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ple^Scer\UAS.cFa does not suppress the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU.

(Humphrey et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ple.PF, ple^UAS.cFa, Scer\GAL4^Ddc.PL rescues ple²

(Riemensperger et al., 2011)

Scer\GAL4^ple.PF/ple^UAS.cFa rescues ple²

(Friggi-Grelin et al., 2003)

Comments

Expression of ple^Scer\UAS.cFa under the control of both Scer\GAL4^ple.PF and Scer\GAL4^Ddc.PL fully rescues (to the adult stage) the embryonic lethality normally found in ple² mutants.

(Riemensperger et al., 2011)

Expression of ple^Scer\UAS.cFa in DA cells under the control of Scer\GAL4^ple.PF leads to full pupal rescue of ple².

(Friggi-Grelin et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ple^Scer\UAS.cFa

ple^UAS.cFa

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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