FlyBase Allele Report: Dmel\hh[GD6242]

General Information

Symbol

Dmel\hh^GD6242

Species

D. melanogaster

Name

FlyBase ID

FBal0209689

Feature type

allele

Associated gene

Dmel\hh

Associated Insertion(s)

Carried in Construct

P{GD6242}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6242}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6242

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^NP2222

(Dong et al., 2021)

abnormal neuroanatomy | larval stage, with Scer\GAL4^repo

(Dong et al., 2021)

decreased cell number | larval stage, with Scer\GAL4^NP2222

(Dong et al., 2021)

decreased occurrence of cell division | larval stage, with Scer\GAL4^NP2222

(Dong et al., 2021)

decreased occurrence of cell division | larval stage, with Scer\GAL4^repo

(Dong et al., 2021)

decreased size | larval stage, with Scer\GAL4^NP2222

(Dong et al., 2021)

decreased size | larval stage, with Scer\GAL4^repo

(Dong et al., 2021)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

brain | larval stage, with Scer\GAL4^NP2222

(Dong et al., 2021)

brain | larval stage, with Scer\GAL4^repo

(Dong et al., 2021)

CNS glial cell | decreased number | larval stage, with Scer\GAL4^NP2222

(Dong et al., 2021)

CNS glial cell | larval stage, with Scer\GAL4^repo

(Dong et al., 2021)

neuroblast | larval stage, with Scer\GAL4^NP2222

(Dong et al., 2021)

neuroblast | larval stage, with Scer\GAL4^repo

(Dong et al., 2021)

Detailed Description

Statement

Reference

The expression of hh^GD6242 under the control of Scer\GAL4^repo (together with Dicer-2 for efficient RNAi) leads to a decrease in CNS volume, with significant disruption of cortex glial chambers; neuroblasts show a decrease in proliferation.

Expression under the control of Scer\GAL4^NP2222 (together with Dicer-2 for efficient RNAi) leads to a decrease in CNS volume, with decreased cortex glial membrane volume and decreased glial cell number; neuroblasts show a decrease in proliferation.

(Dong et al., 2021)

Adults expressing hh^GD6242 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development