FlyBase Allele Report: Dmel\N[UAS.cUa]

General Information

Symbol

Dmel\N^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0215932

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.U}

Also Known As

UAS-N, UAS Notch, UAS-Notch

Key Links

Transgenic product class

wild_type

(Hayward et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hayward et al., 2005)

Mutagen

in vitro construct

(Hayward et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-N.U}

Cytology

Description

UAS regulatory sequences drive expression of full-length N.

(Hayward et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

short lived, with Scer\GAL4^elav.PU, Scer\GAL80^ple.PK

(Imai et al., 2015)

short lived, with Scer\GAL4^ple.PU

(Imai et al., 2015)

Phenotype Manifest In

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

dopaminergic PPM1 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

dopaminergic PPM2 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

dopaminergic PPM3 neuron | adult stage, with Scer\GAL4^ple.PU

(Imai et al., 2015)

Detailed Description

Statement

Reference

Expression of N^Scer\UAS.cUa under the control of Scer\GAL4^ple.PU as well as the Scer\GAL4^elav.PU driver in combination with Scer\GAL80^ple.PK (all together with tub-Gal80[ts] to limit the time of expression to adulthood) results in significantly decreased adult lifespan and, with the Scer\GAL4^ple.PU driver, loss of dopaminergic neurons from the PPL1, PPM1/2 and PPM3 clusters in the adult brain.

(Imai et al., 2015)

N^l1N-ts1 animals raised at the restrictive temperature from 12 hours after puparium formation onwards and expressing N^Scer\UAS.cUa under the control of Scer\GAL4^{neur-GAL4-A101} (to restore N activity selectively in the sensory organs) show normal development of the majority of mechanosensory organs of the leg, and each of these correctly induces a single proximal bract.

(Peng et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

N^UAS.cUa, Scer\GAL4^bbg-C96 is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^bbg-C96, Yif1^GD302

(Kandasamy et al., 2021)

N^UAS.cUa, Scer\GAL4^bbg-C96 is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^bbg-C96, Yif1^GD302

(Kandasamy et al., 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

N^UAS.cUa/Scer\GAL4^hh-Gal4 is an enhancer of phenotype of l(2)gd1^d7

(Schneider et al., 2013)

Suppressor of

Statement

Reference

N^UAS.cUa, Scer\GAL4^bbg-C96 is a suppressor | partially of wing margin phenotype of Scer\GAL4^bbg-C96, Yif1^GD302

(Kandasamy et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^hh-Gal4-mediated expression of N^Scer\UAS.cUa enhances the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

(Schneider et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

N^UAS.cUa/Scer\GAL4^repo-M1B rescues N^l1N-ts1

(Kuzina et al., 2011)

N^UAS.cUa/Scer\GAL4^htl.PM rescues N^l1N-ts1

(Kuzina et al., 2011)

Scer\GAL4^15J2/N^UAS.cUa rescues N^l1N-ts1

(Kuzina et al., 2011)

Partially rescues

N^UAS.cUa/Scer\GAL4^da.G32 partially rescues Df(1)N-54l9

(Lake et al., 2009)

Scer\GAL4^bbg-C96/N^UAS.cUa partially rescues Df(1)N-54l9/+

(Lake et al., 2009)

Comments

Expression of N^Scer\UAS.cUa under the control of Scer\GAL4^repo-M1B efficiently rescues the early axon phenotype of N^l1N-ts1 and restores restores the continuity of the neuronal meshwork.

Expression of N^Scer\UAS.cUa under the control of Scer\GAL4^htl.PM efficiently rescues the early axon phenotype of N^l1N-ts1.

Expression of N^Scer\UAS.cUa under the control of Scer\GAL4^15J2 efficiently rescues the early axon phenotype of N^l1N-ts1.

(Kuzina et al., 2011)

Scer\GAL4^da.G32-mediated expression of N^Scer\UAS.cUa significantly rescues the neurogenic phenotype of Df(1)N-54l9 embryos, and suppresses the notched wing phenotype of Df(1)N-54l9/+ flies.

(Lake et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

N^Scer\UAS.cUa

N^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (13)

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