FlyBase Allele Report: Dmel\Lrp4[KK101962]

General Information

Symbol

Dmel\Lrp4^KK101962

Species

D. melanogaster

Name

FlyBase ID

FBal0231581

Feature type

allele

Associated gene

Dmel\Lrp4

Associated Insertion(s)

Carried in Construct

P{KK101962}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101962}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101962

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^Mz19

(Mosca et al., 2017)

abnormal neuroanatomy | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

abnormal smell perception | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Phenotype Manifest In

adult antennal lobe projection neuron, with Scer\GAL4^Mz19

(Mosca et al., 2017)

adult olfactory receptor neuron, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

presynaptic active zone | adult stage, with Scer\GAL4^Mz19

(Mosca et al., 2017)

presynaptic active zone | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

synapse | adult stage, with Scer\GAL4^Mz19

(Mosca et al., 2017)

synapse | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Detailed Description

Statement

Reference

Expression of Lrp4^KK101962 results in the decreased synapse number (assessed by counting active zone marker puncta) in the VA1v olfactory receptor neurons (with the Scer\GAL4^peb-GAL4 driver) or excitatory projection neurons terminating in the lateral horn (with Scer\GAL4^Mz19) but not in Gad1-positive inhibitory neurons (with the Scer\GAL4^Gad1.3.098 driver) or in the inhibitory projection neurons terminating in the lateral horn (with Scer\GAL4^Mz699), while the neurite volume remains unaffected compared to controls in all cases.

Scer\GAL4^peb-GAL4-driven expression of Lrp4^KK101962 also results in a complete loss of olfactory attraction of adult flies to an attractive odorant (apple cider vinegar) in a trap assay. This lack of olfactory attraction behavior is not due to general defects in locomotion.

(Mosca et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lrp4^KK101962, Scer\GAL4^peb-GAL4 has abnormal smell perception | adult stage phenotype, suppressible | partially by GABA-B-R2^RNAi.UAS, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

NOT suppressed by

Statement

Reference

Lrp4^KK101962, Scer\GAL4^peb-GAL4 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by GABA-B-R2^RNAi.UAS, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Lrp4^KK101962, Scer\GAL4^peb-GAL4 has adult olfactory receptor neuron phenotype, non-suppressible by GABA-B-R2^RNAi.UAS, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Lrp4^KK101962, Scer\GAL4^peb-GAL4 has synapse | adult stage phenotype, non-suppressible by GABA-B-R2^RNAi.UAS, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Lrp4^KK101962, Scer\GAL4^peb-GAL4 has presynaptic active zone | adult stage phenotype, non-suppressible by GABA-B-R2^RNAi.UAS, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of olfactory attraction behavior in response to an attractive odorant (apple cider vinegar) in adult flies expressing Lrp4^KK101962 under the control of Scer\GAL4^peb-GAL4 is ameliorated by co-expression of GABA-B-R2^{dsRNA.Scer\UAS} while the decreased active zone number in olfactory receptor neurons is not suppressed.

(Mosca et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v108629

P{KK101962}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG8909^KK101962

Lrp4^KK101962

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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