FB2024_03 , released June 25, 2024
Allele: Dmel\sdRNAi.N.UAS
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General Information
Symbol
Dmel\sdRNAi.N.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0239883
Feature type
allele
Associated gene
Dmel\sd
Associated Insertion(s)
Carried in Construct
P{UAS-sd.RNAi.N}
Also Known As
UAS-sdRNAi, UAS-Sd-RNAi
Key Links
Transgenic product class
RNAi_reagent
(Zhang et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Zhang et al., 2008)
Mutagen
in vitro construct
(Zhang et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-sd.RNAi.N}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of a fragment encoding sd amino acid residues 36-169.

(Zhang et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
sd
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of sddsRNA.N.Scer\UAS under the control of Scer\GAL4ey.PH (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) results in a small eye phenotype.

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS under the control of Scer\GAL4ey.PH (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) results in a small eye phenotype.

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS under the control of Scer\GAL4hh-Gal4 results in a decrease in size of the posterior compartment of the wing disc.

      (Zhang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS suppresses the overgrowth phenotype seen in wings expressing par-1Scer\UAS.T:Hsap\MYC.cHa under the control of Scer\GAL4Bx-MS1096.

      (Huang et al., 2013)

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with wtsKK101055 in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 suppresses the increase in the number of precursor cells.

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with ykiScer\UAS.cZa in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 suppresses the intestine stem cell proliferation seen upon expression of ykiScer\UAS.cZa alone.

      Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with ykiScer\UAS.cZa in absorptive enterocytes in the adult midgut under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B suppresses the proliferation increase in intestine stem cells seen upon expression of ykiScer\UAS.cZa alone.

      (Ren et al., 2010)

      The enlarged eye phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of either sddsRNA.N.Scer\UAS or sddsRNA.C.Scer\UAS (or both co-expressed together). The ectopic incorporation of BrdU seen in the eye discs of animals expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is also suppressed by simultaneous co-expression of both sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS.

      The eye overgrowth phenotype caused by savSH13 clones is almost completely suppressed by co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS under the control of Scer\GAL4tub.

      The eye overgrowth phenotype caused by hpounspecified clones is almost completely suppressed by co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS under the control of Scer\GAL4tub.

      The eye overgrowth phenotype caused by wtsx1 clones is almost completely suppressed by co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS under the control of Scer\GAL4tub.

      (Zhang et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      sdRNAi.N.UAS
      sddsRNA.N.Scer\UAS
      sddsRNA.N.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (11)