FlyBase Allele Report: Dmel\SREBP[UAS.cKa]

General Information

Symbol

Dmel\SREBP^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kunte

FlyBase ID

FBal0241323

Feature type

allele

Associated gene

Dmel\SREBP

Associated Insertion(s)

Carried in Construct

P{UAS-SREBP.K}

Key Links

Transgenic product class

cDNA

(Kunte et al., 2006)

wild_type

(Kunte et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kunte et al., 2006)

wild_type

(Kunte et al., 2006)

Mutagen

in vitro construct

(Kunte et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-SREBP.K}

Cytology

Description

UASt regulatory sequences drive expression of a full length SREBP cDNA.

(Kunte et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

SREBP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult optic lobe glial cell | cell non-autonomous, with Scer\GAL4^nSyb.PS

(Liu et al., 2017)

lipid droplet | adult stage | cell non-autonomous, with Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Detailed Description

Statement

Reference

Neuronal expression of SREBP^Scer\UAS.cKa (under the control of Scer\GAL4^elav.PU) leads to glial cell specific lipid droplet accumulation. Neuronal expression of SREBP^Scer\UAS.cKa alone does not cause neurodegeneration upon aging and lipid droplets are retained in aged animals. Lipid peroxidation is unchanged in these animals, compared to controls.

Glial expression of SREBP^Scer\UAS.cKa (under the control of Scer\GAL4^54C) does not lead to lipid droplet accumulation.

(Liu et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Sln^GD1940, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Sln^GD1940, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Pdha1^GD12103, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Pdha1^GD12103, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Pdha1^KK101856, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Pdha1^KK101856, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Sln^KK104306, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Sln^KK104306, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by out^GD3448, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by out^GD3448, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by out^KK104187, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by out^KK104187, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Ldh^HMS00039, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

SREBP^UAS.cKa, Scer\GAL4^nSyb.PS has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Ldh^HMS00039, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The accumulation of lipid droplets in the retinal glia of adult flies expressing SREBP^UAS.cKa under the control of Scer\GAL4^nSyb.PS is significantly suppressed by co-expression of any of the following: Sln^GD1940, Sln^KK104306, out^GD3448, out^KK104187, Ldh^HMS00039, Pdha^GD12103 or Pdha^KK101856.

(Liu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^OK376/SREBP^UAS.cKa rescues SREBP¹⁸⁹

(Matthews et al., 2009)

SREBP^UAS.cKa/Scer\GAL4^Switch1.106 rescues SREBP¹⁸⁹

(Kunte et al., 2006)

Scer\GAL4^OK376/SREBP^UAS.cKa rescues SREBP¹⁸⁹

(Kunte et al., 2006)

SREBP^GAL4::VP16/SREBP^UAS.cKa rescues SREBP¹⁸⁹

(Kunte et al., 2006)

Partially rescues

Scer\GAL4^DcG/SREBP^UAS.cKa partially rescues SREBP¹⁸⁹

(Kunte et al., 2006)

Fails to rescue

Scer\GAL4^c179/SREBP^UAS.cKa fails to rescue SREBP¹⁸⁹

(Kunte et al., 2006)

Comments

Expression of HLH106^Scer\UAS.cKa under the control of Scer\GAL4^OK376 in a HLH106¹⁸⁹ mutant background rescues lethality the majority of animals.

(Matthews et al., 2009)

Expression of HLH106^Scer\UAS.cKa under the control of Scer\GAL4^Switch1.106 in the presence of RU486 allows HLH106¹⁸⁹ homozygotes to survive to pupariation and emerge as adults. The rescued adults survive at least two weeks in the absence of RU486.

(Kunte et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38396

w^*; P{UAS-SREBP.K}2/CyO; SREBP¹⁸⁹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

HLH106^Scer\UAS.cKa

SREBP^Scer\UAS.cKa

SREBP^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kunte

Secondary FlyBase IDs

References (4)

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