FB2024_03 , released June 25, 2024
Allele: Dmel\SREBP189
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General Information
Symbol
Dmel\SREBP189
Species
D. melanogaster
Name
FlyBase ID
FBal0241326
Feature type
allele
Associated gene
Dmel\SREBP
Associated Insertion(s)
Carried in Construct
Also Known As
dSREBP189, dSREP189, SREBPΔ189
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Kunte et al., 2006)
Progenitor genotype
P{SUPor-P}Gyc76CKG03723
(Kunte et al., 2006)
Caused by aberration
Df(3L)HLH106189
Cytology
Description

Imprecise excision of the progenitor insertion has resulted in a deletion which affects both Gyc76C and HLH106 (neighbouring genes). The deletion extends 697bp into the HLH106 open reading frame, up to amino acid residue 233 in exon 3 (the next in-frame start codon is at amino acid residue 265). The deletion also removes the predicted first exon of Gyc76C.

(Kunte et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:19,793,069..19,795,606 [+]
Comment:

Deletion extends from site of P{SUPor-P}Gyc76CKG03723 insertion within Gyc76C to amino acid 233 of SREBP. The deletion removes 697 nucleotides of the SREBP ORF and the first noncoding exon of Gyc76C.

(Kunte et al., 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  heart conduction disease
      modeled by eas2
      (Lim et al., 2011)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      HLH106189 mutants survive extremely poorly on unsupplemented medium, and their survival is much improved on medium supplemented with fatty acids.

      (Amarneh et al., 2009)

      In HLH106189 mutant flies, lethality is reduced by supplementing food with fatty acids.

      (Matthews et al., 2009)

      Homozygous larvae show a profound defects in growth during the second larval instar.

      HLH10652/HLH106189 first instar larvae contain significantly less total fatty acid than wild-type controls, but the relative abundance of various fatty acid species does not differ significantly from that of controls.

      The survival of homozygotes to adulthood is markedly improved in the presence of soy lipids (lecithin) in the food medium, and increases with increasing concentration of soy lipid (up to 9%). The rescued adults are indistinguishable in mass from their heterozygous siblings and are morphologically normal.

      (Kunte et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      HLH106[+]/SREBP189, SREBPD386A/SREBPD386A is an enhancer of lethal | larval stage phenotype of S2PKG08356/S2P1

      (Amarneh et al., 2009)

      SREBPD386A, SREBPD386A, SREBP189, SREBP189 is an enhancer of lethal | larval stage phenotype of S2PKG08356/S2P1

      (Amarneh et al., 2009)
      Suppressor of
      Statement
      Reference

      SREBP[+]/SREBP189 is a suppressor of decreased cell number | adult stage | progressive phenotype of mir-210Δ130

      (Lyu et al., 2021)
      NOT Suppressor of
      Statement
      Reference

      SREBP[+]/SREBP189 is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage | progressive | conditional phenotype of Pectomb593

      (Tsai et al., 2019)

      HLH106[+]/SREBP189, SREBPD386A/SREBPD386A is a non-suppressor of viable | conditional phenotype of S2PKG08356/S2P1

      (Amarneh et al., 2009)

      SREBPD386A, SREBPD386A, SREBP189, SREBP189 is a non-suppressor of viable | conditional phenotype of S2PKG08356/S2P1

      (Amarneh et al., 2009)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      SREBP[+]/SREBP189 is a suppressor of eye photoreceptor cell | adult stage | decreased number phenotype of mir-210Δ130

      (Lyu et al., 2021)

      SREBP[+]/SREBP189 is a suppressor of retina | adult stage phenotype of mir-210Δ130

      (Lyu et al., 2021)

      SREBP[+]/SREBP189 is a suppressor of lipid droplet | increased number | adult stage phenotype of mir-210Δ130

      (Lyu et al., 2021)

      SREBP[+]/SREBP189 is a suppressor of rhabdomere | adult stage | decreased number phenotype of mir-210Δ130

      (Lyu et al., 2021)
      NOT Suppressor of
      Statement
      Reference

      SREBP[+]/SREBP189 is a non-suppressor of axon | somatic clone | adult stage | progressive | conditional phenotype of Pectomb593

      (Tsai et al., 2019)

      SREBP[+]/SREBP189 is a non-suppressor of eye photoreceptor cell | somatic clone | adult stage | progressive | conditional phenotype of Pectomb593

      (Tsai et al., 2019)

      SREBP[+]/SREBP189 is a non-suppressor of optic cartridge | somatic clone | adult stage | progressive | conditional phenotype of Pectomb593

      (Tsai et al., 2019)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A SREBP189 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in sicilyE mutants.

      A SREBP189 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in Aats-metFB mutants.

      A SREBP189 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in MarfB mutants.

      (Liu et al., 2015)

      On unsupplemented medium, S2PKG08356/S2P1 mutants in the presence of two copies of HLH106D386A and carrying HLH106189/+, survive at less than 25% of the expected rate. When these animals are homozygous for HLH106189, survival at only 10% of the expected rate is observed. Survival is restored by supplementing the culture medium with free fatty acids.

      (Amarneh et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      SREBP189 is rescued by Scer\GAL4OK376/SREBPUAS.cKa

      (Matthews et al., 2009)

      SREBP189 is rescued by Scer\GAL4OK376/SREBPNP-FL.UAS

      (Matthews et al., 2009)

      SREBP189 is rescued by SREBPUAS.cKa/Scer\GAL4Switch1.106

      (Kunte et al., 2006)

      SREBP189 is rescued by Scer\GAL4OK376/SREBPUAS.cKa

      (Kunte et al., 2006)

      SREBP189 is rescued by SREBPGAL4::VP16/SREBPUAS.cKa

      (Kunte et al., 2006)

      SREBP189 is rescued by SREBP+t8.7

      (Kunte et al., 2006)
      Partially rescued by

      SREBP189 is partially rescued by Scer\GAL4DcG/SREBPUAS.cKa

      (Kunte et al., 2006)
      Not rescued by

      SREBP189 is not rescued by Scer\GAL4c179/SREBPUAS.cKa

      (Kunte et al., 2006)
      Comments

      Expression of HLH106Scer\UAS.cKa under the control of Scer\GAL4OK376 in a HLH106189 mutant background rescues lethality the majority of animals.

      Expression of HLH106NP-FL.Scer\UAS under the control of Scer\GAL4OK376 in a HLH106189 mutant background rescues lethality in approximately 50% of animals.

      (Matthews et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (5)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      HLH106189
      SREBP Δ189
      (Tsai et al., 2019)
      SREBP189
      (Luo et al., 2020)
      SREBPΔ189
      (Lyu et al., 2021, Sieber and Spradling, 2015)
      Srebp189
      (Mariano et al., 2023)
      dSREBP189
      (Lim et al., 2011, Matthews et al., 2010, Kunte et al., 2006)
      dSREP189
      (Amarneh et al., 2009, Matthews et al., 2009)
      srebp189
      (Meltzer et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (13)