FlyBase Allele Report: Dmel\SREBP[GAL4::VP16]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\SREBP^GAL4::VP16

General Information

Symbol

Dmel\SREBP^GAL4::VP16

Species

D. melanogaster

Name

FlyBase ID

FBal0241325

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{GAL4-dSREBPg.K}

Also Known As

P{GAL4-dSREBPg}, SREBP-Gal4, Gal4-SREBP

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Kunte et al., 2006)

Progenitor genotype

(Kunte et al., 2006)

Carried in construct

P{GAL4-dSREBPg.K}

Cytology

Description

bHLH domain of SREBP has been replaced by sequence encoding a GAL4::VP16 driver in the context of a genomic fragment that is expressed under the control of the native SREBP promoter. The protein produced is subject to the same physiologically-regulated proteolytic processing as wild-type SREBP. Thus, this construct acts as a reporter to follow SREBP protein cleavage in vivo.

(Kunte et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

genetically encoded sensor

Tagged with

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell size | larval stage, with Axn¹²⁷, Axn^HMJ23888

(Zhang et al., 2017)

visible | larval stage, with Axn¹²⁷, Axn^HMJ23888

(Zhang et al., 2017)

Phenotype Manifest In

larval abdomen, with Axn¹²⁷, Axn^HMJ23888

(Zhang et al., 2017)

larval fat cell, with Axn¹²⁷, Axn^HMJ23888

(Zhang et al., 2017)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor | partially of abnormal cell size | larval stage phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/pan^JF02306 is a suppressor of visible | larval stage phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/lgs^GL00619 is a suppressor of visible | larval stage phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor of visible | larval stage phenotype of Axn¹²⁷

(Zhang et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

SREBP^GAL4::VP16/pan^JF02306 is a suppressor of larval abdomen phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/lgs^GL00619 is a suppressor of larval abdomen phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor of larval abdomen phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor | partially of larval fat cell phenotype of Axn¹²⁷

(Zhang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

SREBP^GAL4::VP16/Axn^EY10228 rescues Axn^EY10228/Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/SREBP^UAS.cKa rescues SREBP¹⁸⁹

(Kunte et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

38395

y¹ w^*; P{GAL4-dSREBPg.K}A39; SREBP¹⁸⁹/TM6B, Tb¹

38394

y¹ w^*; wg^Sp-1/CyO; P{GAL4-dSREBPg.K}A45/TM6B, Tb⁺

39612

w^*; Df(2R)SCAP^Δ910/CyO, P{ActGFP}JMR1; P{GAL4-dSREBPg.K}B31, P{UAS-GFP.U}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

HLH106^{T:Scer\GAL4,T:Hsim\VP16}

SREBP^GAL4::VP16

SREBP^{T:Scer\GAL4,T:Hsim\VP16}

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections