FlyBase Allele Report: Dmel\pico[RNAi.UAS]

General Information

Symbol

Dmel\pico^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0243035

Feature type

allele

Associated gene

Dmel\pico

Associated Insertion(s)

Carried in Construct

P{UAS-pico.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Lyulcheva et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Lyulcheva et al., 2008)

Mutagen

in vitro construct

(Lyulcheva et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-pico.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a tail-to-tail inverted 477bp inverted repeat of pico, corresponding to 17-493 bp of the coding sequence.

(Lyulcheva et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

pico

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size, with Scer\GAL4^Tub.PU

(Lyulcheva et al., 2008)

decreased cell number, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

decreased cell number, with Scer\GAL4^ptc-559.1

(Lyulcheva et al., 2008)

decreased cell number | somatic clone, with Scer\GAL4^Act5C.PU

(Lyulcheva et al., 2008)

decreased cell size, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

partially lethal - majority die, with Scer\GAL4^Tub.PU

(Lyulcheva et al., 2008)

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

wing, with Scer\GAL4^en-e16E

(Lyulcheva et al., 2008)

wing blade dorsal compartment, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

Detailed Description

Statement

Reference

Expression of pico^IR.Scer\UAS under the control of Scer\GAL4^tub is semilethal, and survivors are small compared to sibling controls.

Expression of pico^IR.Scer\UAS in the wing under the control of Scer\GAL4^Bx-MS1096 results in a significant reduction in wing area. Fewer and smaller wing cells are seen, and wing hair density is significantly increased relative to wildtype. The dorsal wing blade is reduced in size, and wings are slightly curled upwards.

Expression of pico^IR.Scer\UAS in the posterior compartment of the wing disc under the control of Scer\GAL4^en-e16E results in a specific reduction in the area of the posterior wing compartment. Cells progress through the cell cycle normally, but are smaller in size than controls.

Somatic homozygous pico^IR.Scer\UAS mutant clones (driven by Scer\GAL4^Act5C) contain significantly fewer cells than control clones, and therefore contains cells that divide at a slower rate.

Expressing pico^IR.Scer\UAS in a stripe of cells in the wing using Scer\GAL4^ptc-559.1 results in a reduction in the number of cells and a narrower stripe.

(Lyulcheva et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

pico^RNAi.UAS, Scer\GAL4^vg.PU is a suppressor of wing margin phenotype of Ras85D^V12.UAS, Scer\GAL4^vg.PU

(Lyulcheva et al., 2008)

pico^RNAi.UAS is a suppressor of wing phenotype of Egfr^UAS.cBa, Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

pico^RNAi.UAS is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, ena^{UAS.Tag:polyHis}

(Lyulcheva et al., 2008)

NOT Suppressor of

Statement

Reference

pico^RNAi.UAS is a non-suppressor of wing phenotype of Mrtf^UAS.cSa, Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of pico^IR.Scer\UAS suppresses the wing overgrowth seen when Egfr^Scer\UAS.cBa is expressed in the wing using Scer\GAL4^Bx-MS1096.

The wing notching that results from expressing Ras85D^V12.Scer\UAS along the wing margin using Scer\GAL4^vg.PU is partially suppressed by co-expression of pico^IR.Scer\UAS.

(Lyulcheva et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pico^RNAi.UAS is rescued by pico^{UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

Rescues

Scer\GAL4^Bx-MS1096/pico^RNAi.UAS rescues pico^{UAS.Tag:polyHis,Tag:MYC}

(Lyulcheva et al., 2008)

Comments

Over expression of pico^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} and pico^IR.Scer\UAS simultaneously in the wing by Scer\GAL4^Bx-MS1096 results in normal wing formation, and the phenotypic effects of each alone are nullified.

(Lyulcheva et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

pico^IR.Scer\UAS

pico^RNAi.UAS

pico^{dsRNA.Scer\UAS}

pico^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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