FlyBase Allele Report: Dmel\CycB[HMS01015]

General Information

Symbol

Dmel\CycB^HMS01015

Species

D. melanogaster

Name

FlyBase ID

FBal0257682

Feature type

allele

Associated gene

Dmel\CycB

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01015}

Also Known As

UAS-CycB^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01015}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01015

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Delta^UAS.cDa, Not3^KK102144

(Vicente et al., 2018)

modeled by Delta^UAS.cDa, twin^KK108897

(Vicente et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^c768

(Zhou and Luo, 2013)

Phenotype Manifest In

egg chamber, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female fusome, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female germline cyst, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female germline stem cell, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

larval outer optic anlage, with Scer\GAL4^c768

(Zhou and Luo, 2013)

Detailed Description

Statement

Reference

Knockdown of CycB, through expression of CycB^HMS01015 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} generates germline stem cells with many characteristics of cyst cells, such as branched fusomes (in 6.25% of egg chambers) and resulting egg chambers made of 32 cells, as well as the expected eight-cell cysts.

(Mathieu et al., 2013)

Expression of CycB^HMS01015 under the control of Scer\GAL4^c768 results defects in maintenance of the neuroepithelium cells in the developing optic lobe and premature neuroblast formation. There is an increase in mitotic neuroepithelium cells and a reduction in BrdU incorporation compared to wild type.

(Zhou and Luo, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CycB^HMS01015, Scer\GAL4^GMR28E03 is a suppressor | partially of increased occurrence of cell division | adult stage phenotype of Myt1^KK102559, Scer\GAL4^GMR28E03

(Willms et al., 2020)

CycB^HMS01015, Scer\GAL4^ey.PH is a suppressor | partially of visible | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897