FlyBase Allele Report: Dmel\Ldh[KK102330]

General Information

Symbol

Dmel\Ldh^KK102330

Species

D. melanogaster

Name

FlyBase ID

FBal0259161

Feature type

allele

Associated gene

Dmel\Ldh

Associated Insertion(s)

Carried in Construct

P{KK102330}

Also Known As

UAS-LDH^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK102330}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102330

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Mattila et al., 2018)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP0001

(Iatsenko et al., 2018)

Phenotype Manifest In

adult gut, with Scer\GAL4^NP0001

(Iatsenko et al., 2018)

adult midgut | somatic clone, with Scer\GAL4^Tub.PU

(Mattila et al., 2018)

Detailed Description

Statement

Reference

Expression of Ldh^KK102330 under the control of Scer\GAL4^NP0001 in axenic flies (colonized with Lp isolated from PGRP-SD^sk1 mutants) leads to a decreased occurrence of cell division while it does not lead to any changes in the lifespan of flies when compared to controls.

(Iatsenko et al., 2018)

Number of midgut cells per MARCM clone expressing Ldh^KK102330 under the control of Scer\GAL4^Tub.PU is significantly less than control adults under calorie-restricted condition.

(Mattila et al., 2018)

Expression of ImpL3^KK102330 under the control of Scer\GAL4^en.PU does not result in a significant change in size of the third instar wing disc posterior compartment, as compared to controls.

(Slaninova et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ldh^KK102330, Scer\GAL4^NP0001 has decreased occurrence of cell division | adult stage phenotype, suppressible | partially by PGRP-SD^sk1/PGRP-SD^sk1

(Iatsenko et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^NP0001/Ldh^KK102330 is a suppressor of increased occurrence of cell division | adult stage phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Scer\GAL4^NP0001/Ldh^KK102330 is a suppressor | partially of short lived phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Ldh^KK102330, Scer\GAL4^ptc.PU is a suppressor | partially of hyperplasia | third instar larval stage phenotype of N^UAS.icd, Scer\GAL4^ptc.PU

(Slaninova et al., 2016)

Other

Statement

Reference

Glut1^KK108683, Ldh^KK102330, Scer\GAL4^en.PU has abnormal size | third instar larval stage phenotype

(Slaninova et al., 2016)

Hex-A^KK100831, Ldh^KK102330, Scer\GAL4^en.PU has abnormal size | third instar larval stage phenotype

(Slaninova et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ldh^KK102330, Scer\GAL4^NP0001 has adult gut phenotype, suppressible | partially by PGRP-SD^sk1/PGRP-SD^sk1

(Iatsenko et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^NP0001/Ldh^KK102330 is a suppressor of adult gut phenotype of PGRP-SD^sk1

(Iatsenko et al., 2018)

Ldh^KK102330, Scer\GAL4^ptc.PU is a suppressor | partially of wing disc | third instar larval stage phenotype of N^UAS.icd, Scer\GAL4^ptc.PU

(Slaninova et al., 2016)

Other

Statement

Reference

Glut1^KK108683, Ldh^KK102330, Scer\GAL4^en.PU has wing disc posterior compartment | third instar larval stage phenotype

(Slaninova et al., 2016)

Hex-A^KK100831, Ldh^KK102330, Scer\GAL4^en.PU has wing disc posterior compartment | third instar larval stage phenotype

(Slaninova et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ImpL3^KK102330 and Glut1^KK108683, or ImpL3^KK102330 and Hex-A^KK100831, under the control of Scer\GAL4^en.PU results in a significant decrease in size of the third instar wing disc posterior compartment, as compared to controls.

Co-expression of ImpL3^KK102330 partially rescues the wing disc hyperplasia seen in larvae expressing N^Scer\UAS.icd under the control of Scer\GAL4^ptc.PU.

(Slaninova et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v110190

P{KK102330}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ImpL3^KK102330

Ldh^KK102330

dLDH^RNAi-1

(Charlton-Perkins et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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