FB2024_03 , released June 25, 2024
Allele: Dmel\PGRP-SDsk1
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General Information
Symbol
Dmel\PGRP-SDsk1
Species
D. melanogaster
Name
FlyBase ID
FBal0325049
Feature type
allele
Associated gene
Dmel\PGRP-SD
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Iatsenko et al., 2016)
Progenitor genotype
Cytology
Description

11bp deletion in PGRP-SD (nucleotides 129-139); this induces a frameshift, causing a premature stop codon and leading to a peptide of 50 residues lacking the PGRP domain.

(Iatsenko et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:7,651,333..7,651,343 [+]
Comment:

11 bp deletion at the 5' end of PGRP-SD causes a frameshift and early translation termination.

(Iatsenko et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      PGRP-SDsk1/PGRP-SDsk1 and PGRP-SDsk1/Df(3L)BSC388 adults exhibit increased mortality upon exposure to Pseudomonas entomophila oral infection when compared to controls.

      Conventionally raised and axenic (grown in antibiotics-supplemented food) PGRP-SDsk1 homozygous adults exhibit significantly shorter lifespan when compared to controls.

      Conventionally raised (old but not young) PGRP-SDsk1 homozygous adults exhibit increased proliferation of intestinal stem cells while old axenic homozygotes exhibit normal proliferation of intestinal stem cells when compared to controls.

      PGRP-SDsk1 homozygous adults harbor more L. plantarum (Lp, the predominant type of bacteria in mutants and controls) than controls.

      Axenic PGRP-SDsk1 homozygous adults colonized with Lp[SD] (Lp isolated from conventionally raised PGRP-SDsk1 homozygotes) exhibit significantly increased occurrence of cell division in gut and significantly decreased lifespan when compared to controls.

      PGRP-SDsk1 homozygous adults that are microbiota transplanted either with their native microbiota or with microbiota isolated from controls exhibit significantly decreased lifespan when compared to controls.

      Axenic PGRP-SDsk1 homozygous adults colonized with A. pomorum do not exhibit any changes in their lifespan, in the amount of A. pomorum they harbor or in the occurrence of cell division in gut when compared to controls.

      PGRP-SDsk1 homozygosity leads to an increase in the proportion of adults with acidic guts in conventionally raised and old (but not young) flies when compared to controls. This increase is reduced in old axenic adults.

      PGRP-SDsk1 homozygosity leads to an increase in lactate concentration in gut of conventionally raised (but not axenic) and old (but not young) flies when compared to controls.

      In axenic PGRP-SDsk1 homozygous adults colonized with Lp or a mutant strain of Lp (Lp[TF103], persists in the gut in similar quantities as Lp) the proportion of flies with acidic guts is increased, the adult lifespan is significantly decreased and the occurrence of cell division in gut is increased (only significantly in flies with Lp) when compared to controls. The flies with Lp exhibit more severe phenotypes than the flies with Lp[TF103].

      (Iatsenko et al., 2018)

      PGRP-SDsk1 mutant flies are viable and fertile, their adult lifespan at 29[o]C is comparable to wild-type and they do not display any defects in bacterial elimination upon infection with either Gram-negative Erwinia carotovora or Gram-positive Listeria monocytogenes. They also do not exhibit any increased sensitivity to infections with bacteria Enterococcus faecalis, Streptococcus pyogenes or Erwinia carotovora or fungi Beauveria bassiana and Aspergillus fumigatus but show reduced survival rate upon infection with either Pseudomonas entomophila or Bacillus subtilis relative to wild-type controls.

      (Iatsenko et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      NOT Enhancer of
      Suppressor of
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      PGRP-SDsk1 has adult gut phenotype, non-enhanceable by Scer\GAL4NP0001/DuoxRNAi.UAS

      (Iatsenko et al., 2018)
      Suppressed by
      Statement
      Reference

      PGRP-SDsk1 has adult gut phenotype, suppressible | partially by Scer\GAL4NP0001/NoxGD2030

      (Iatsenko et al., 2018)

      PGRP-SDsk1 has adult gut phenotype, suppressible by Scer\GAL4NP0001/LdhKK102330

      (Iatsenko et al., 2018)
      NOT suppressed by
      Statement
      Reference

      PGRP-SDsk1 has adult gut phenotype, non-suppressible by Scer\GAL4NP0001/DuoxRNAi.UAS

      (Iatsenko et al., 2018)
      Suppressor of
      Statement
      Reference

      PGRP-SDsk1/PGRP-SDsk1 is a suppressor | partially of adult gut phenotype of LdhKK102330, Scer\GAL4NP0001

      (Iatsenko et al., 2018)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The reduced survival rate of either PGRP-SAseml or GNBP1e03371 mutant adults upon infection with either Staphylococcus aureus or Streptococcus pyogenes is not worsened further by combination with PGRP-SDsk1.

      The decreased lifespan at 29[o]C as well as the precocious lethality upon Erwinia carotovora infection characteristic for PGRP-LBΔ mutant adults is rescued in PGRP-LBΔ;PGRP-SDsk1 double mutants.

      (Iatsenko et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PGRP-SDSK1
      (Iatsenko et al., 2020)
      PGRP-SDsk1
      (Iatsenko et al., 2018, Iatsenko et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)