FlyBase Allele Report: Dmel\Tg[A.UAS]

General Information

Symbol

Dmel\Tg^A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0264822

Feature type

allele

Associated gene

Dmel\Tg

Associated Insertion(s)

Carried in Construct

P{UAS-Tg.A}

Also Known As

UAS-dTG-A

Key Links

Transgenic product class

wild_type

(Umehara et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Umehara et al., 2010)

Mutagen

in vitro construct

(Umehara et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Tg.A}

Cytology

Description

UASt regulatory sequences drive expression of 2.3kb of Tg isoform A coding sequences.

(Umehara et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 6

modeled by Pink1^UAS.Tag:HA

(Min et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PU

(Min et al., 2015)

Phenotype Manifest In

crossvein, with Scer\GAL4^c179

(Ichikawa et al., 2011)

crossvein, with Scer\GAL4^dpp.blk1

(Ichikawa et al., 2011)

crossvein, with Scer\GAL4^en.PU

(Ichikawa et al., 2011)

crossvein, with Scer\GAL4^ptc-559.1

(Ichikawa et al., 2011)

eye, with Scer\GAL4^GMR.PS

(Ichikawa et al., 2011, Umehara et al., 2010)

eye, with Scer\GAL4^GMR.PU

(Min et al., 2015)

interommatidial bristle, with Scer\GAL4^GMR.PS

(Umehara et al., 2010)

ommatidium, with Scer\GAL4^GMR.PS

(Umehara et al., 2010)

Detailed Description

Statement

Reference

Expression of Tg^A.Scer\UAS driven by Scer\GAL4^GMR.PU results in a mild rough eye phenotype (disrupted ommatidial array organisation and bristle loss).

(Min et al., 2015)

Expression of Tg^A.Scer\UAS in the region posterior to the morphogenic furrow in the eye imaginal disc, under the control of Scer\GAL4^GMR.PS or in the posterior compartment of the wing imaginal disc, under the control of Scer\GAL4^en.PU, causes a rough eye phenotype in the compound eyes and an extra wing crossvein phenotype in the wings.

Expression of Tg^A.Scer\UAS mainly in the border between the anterior and the posterior compartment of the wing imaginal disc, under the control of Scer\GAL4^dpp.blk1, causes an extra wing crossvein phenotype in and around the border.

Expression of Tg^A.Scer\UAS mainly in the border between the anterior and the posterior compartment of the wing imaginal disc, under the control of Scer\GAL4^ptc-559.1, results in an extra wing crossvein phenotype.

Expression of Tg^A.Scer\UAS mainly in the wing pouch of the wing imaginal disc, under the control of Scer\GAL4^c179, results in an extra wing crossvein phenotype.

Expression of Tg^A.Scer\UAS under the control of Scer\GAL4^ey.PU, Scer\GAL4^Dll.PU, Scer\GAL4^Bx-MS1096, Scer\GAL4^pnr-MD237, Scer\GAL4^Cg.PA or Scer\GAL4^69B does not generate any morphological change.

(Ichikawa et al., 2011)

Expression of Tg^A.Scer\UAS with Scer\GAL4^GMR.PS causes a rough eye phenotype in adult eyes. The ommatidia lack their regular hexagonal shape and some missing bristles are apparent. This phenotype is repressed at low temperatures (e.g. 19[o]C).

Expression of Tg^A.Scer\UAS under the control of Scer\GAL4^ey.PH, Scer\GAL4^Dll-md23, Scer\GAL4^Bx-MS1096, Scer\GAL4^sd.PU, Scer\GAL4^pnr-MD237, Scer\GAL4^Cg.PA or Scer\GAL4^69B does not cause any apparent morphological changes.

(Umehara et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Tg^A.UAS has visible phenotype, enhanceable by Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Min et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Tg^A.UAS has visible phenotype, suppressible by Pink1^NIG.4523R, Scer\GAL4^GMR.PU

(Min et al., 2015)

Enhancer of

Statement

Reference

Tg^A.UAS, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Min et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Tg^A.UAS has eye phenotype, enhanceable by Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Min et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Tg^A.UAS has eye phenotype, suppressible by Pink1^NIG.4523R, Scer\GAL4^GMR.PU

(Min et al., 2015)

Scer\GAL4^GMR.PS, Tg^A.UAS has eye phenotype, suppressible by bsk¹/bsk[+]

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has ommatidium phenotype, suppressible by bsk¹/bsk[+]

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has ommatidium phenotype, suppressible by hep[+]/hep^r75

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has interommatidial bristle phenotype, suppressible by hep[+]/hep^r75

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has ommatidium phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has interommatidial bristle phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has interommatidial bristle phenotype, suppressible by bsk¹/bsk[+]

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has eye phenotype, suppressible by bsk²/bsk[+]

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has ommatidium phenotype, suppressible by bsk²/bsk[+]

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has interommatidial bristle phenotype, suppressible by bsk²/bsk[+]

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has eye phenotype, suppressible by hep[+]/hep¹

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has ommatidium phenotype, suppressible by hep[+]/hep¹

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has interommatidial bristle phenotype, suppressible by hep[+]/hep¹

(Umehara et al., 2010)

Scer\GAL4^GMR.PS, Tg^A.UAS has eye phenotype, suppressible by hep[+]/hep^r75

(Umehara et al., 2010)

Enhancer of

Statement

Reference

Tg^A.UAS, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Min et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Tg^A.Scer\UAS and Pink1^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^GMR.PU results in enhanced rough eye phenotypes compared to expression of either construct alone. Co-expression of Pink1^NIG.4523R suppresses the mild rough eye phenotype seen in flies with Tg^A.Scer\UAS driven by Scer\GAL4^GMR.PU.

(Min et al., 2015)

A bsk¹/+, bsk²/+, hep^r75/+, or a hep¹/+ background suppressed the rough eye phenotype found upon expression of Tg^A.Scer\UAS under the control of Scer\GAL4^GMR.PS.

(Umehara et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa, suppresses the rough eye phenotype induced by the overexpression of Tg^A.Scer\UAS in the eye imaginal disc under the control of Scer\GAL4^GMR.PS.

(Umehara et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tg^A.Scer\UAS

Tg^A.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close