FlyBase Allele Report: Dmel\flw[FP41]

General Information

Symbol

Dmel\flw^FP41

Species

D. melanogaster

Name

FlyBase ID

FBal0267524

Feature type

allele

Associated gene

Dmel\flw

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Sun et al., 2011)

Progenitor genotype

Cytology

Description

A single point mutation results in replacement of an aspartic acid for a asparagine at amino acid 222 in orb2 isoform A and at amino acid 91 in isoform B.

(Sun et al., 2011)

Amino acid replacement: D222N.

(Sun et al., 2011)

Amino acid replacement: D91N.

(Sun et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:10,406,571..10,406,571 [+]

Nucleotide change:

G10406571A

Amino acid change:

D222N | flw-PA; D91N | flw-PB; D91N | flw-PC; D91N | flw-PD

Reported amino acid change:

D222N, D91N

Comment:

The mutation was reported at position 222 relative to transcript RA and position 91 relative to transcript R-B.

(Sun et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | recessive | somatic clone

(Sun et al., 2011)

increased cell number | recessive | somatic clone

(Sun et al., 2011)

increased occurrence of cell division | female | somatic clone

(Sun et al., 2011)

lethal - all die before end of larval stage

(Sun et al., 2011)

some die during larval stage

(Sun et al., 2011)

Phenotype Manifest In

follicle cell | posterior | somatic clone

(Sun et al., 2011)

nurse cell ring canal | germline clone

(Yamamoto et al., 2013)

oocyte | somatic clone

(Sun et al., 2011)

Detailed Description

Statement

Reference

Homozygous female germline clones result in small actin ring canals.

(Yamamoto et al., 2013)

When posterior follicle cells (PFCs) are homozygous mutant for flw^FP41 the oocyte nucleus often fails to relocalise and remains at the posterior pole. Oocyte polarity is unaffected when PFCs are wild-type and the anterior or lateral follicle cells are mutant for flw^FP41. Some of the cells found in flw^FP41 mutant PFC clones have small nuclei compared to controls and occasionally multiple cell layers are present at the posterior. Unlike in wild-type, mitotic PH3 positive PFCs can still be seen after stage 6. This overproliferation phenotype is never observed in clones induced at the lateral side of the epithelium.

(Sun et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

flw^FP41 has increased cell number | recessive | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PU/btl::Egfr^λ.UAS

(Sun et al., 2011)

flw^FP41 has increased cell number | recessive | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PU/upd1^UAS.cZa

(Sun et al., 2011)

Suppressed by

Statement

Reference

flw^FP41 has abnormal cell polarity | recessive | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act5C.PU/N^ICN.UAS

(Sun et al., 2011)

flw^FP41 has abnormal cell polarity | recessive | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act5C.PU/N^ECN.UAS.cGa

(Sun et al., 2011)

flw^FP41 has abnormal cell polarity | recessive | somatic clone phenotype, suppressible | partially by zip[+]/zip¹

(Sun et al., 2011)

flw^FP41 has abnormal cell polarity | recessive | somatic clone phenotype, suppressible | partially by Rho1^E3.10/Rho1[+]

(Sun et al., 2011)

flw^FP41 has abnormal cell polarity | recessive | somatic clone phenotype, suppressible | partially by RhoGEF2⁰⁴²⁹¹/RhoGEF2[+]

(Sun et al., 2011)

flw^FP41 has abnormal cell polarity | recessive | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act5C.PU/N^UAS.cSb

(Sun et al., 2011)

Other

Statement

Reference

Scer\GAL4^Act5C.PU, btl::Egfr^λ.UAS, flw^FP41 has increased cell number | recessive | somatic clone phenotype

(Sun et al., 2011)

Scer\GAL4^Act5C.PU, flw^FP41, upd1^UAS.cZa has increased cell number | recessive | somatic clone phenotype

(Sun et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

flw^FP41 has follicle cell | posterior | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PU/btl::Egfr^λ.UAS

(Sun et al., 2011)

flw^FP41 has follicle cell | posterior | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PU/upd1^UAS.cZa

(Sun et al., 2011)

Suppressed by

Statement

Reference

flw^FP41 has oocyte | somatic clone phenotype, suppressible | partially by zip[+]/zip¹

(Sun et al., 2011)

flw^FP41 has oocyte | somatic clone phenotype, suppressible | partially by Rho1^E3.10/Rho1[+]

(Sun et al., 2011)

flw^FP41 has oocyte | somatic clone phenotype, suppressible | partially by RhoGEF2⁰⁴²⁹¹/RhoGEF2[+]

(Sun et al., 2011)

flw^FP41 has oocyte | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act5C.PU/N^UAS.cSb

(Sun et al., 2011)

flw^FP41 has oocyte | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act5C.PU/N^ICN.UAS

(Sun et al., 2011)

flw^FP41 has oocyte | somatic clone phenotype, suppressible | partially by Scer\GAL4^Act5C.PU/N^ECN.UAS.cGa

(Sun et al., 2011)

Other

Statement

Reference

Scer\GAL4^Act5C.PU, btl::Egfr^λ.UAS, flw^FP41 has follicle cell | anterior | somatic clone phenotype

(Sun et al., 2011)

Scer\GAL4^Act5C.PU, flw^FP41, upd1^UAS.cZa has follicle cell | anterior | somatic clone phenotype

(Sun et al., 2011)

Scer\GAL4^Act5C.PU, flw^FP41, upd1^UAS.cZa has follicle cell | lateral | somatic clone phenotype

(Sun et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of zip¹ partially suppresses the oocyte polarity defects seen when the posterior follicle cells are mutant for flw^FP41.

One copy of Rho1^E3.10 partially suppresses the oocyte polarity defects seen when the posterior follicle cells are mutant for flw^FP41.

One copy of RhoGEF2⁰⁴²⁹¹ partially suppresses the oocyte polarity defects seen when the posterior follicle cells are mutant for flw^FP41.

Expression of N^Scer\UAS.cSb under the control of Scer\GAL4^Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flw^FP41.

Expression of N^ICN.Scer\UAS under the control of Scer\GAL4^Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flw^FP41.

Expression of N^{ECN.Scer\UAS.cGa} under the control of Scer\GAL4^Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flw^FP41.

Expression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^Act5C.PU in flw^FP41 mutant follicle cell clones results in overproliferation in anterior follicle cells but not in lateral follicle cells. The amount of cell overproliferation in posterior follicle cells is increased compared to flw^FP41 clones alone.

Expression of upd1^Scer\UAS.cZa under the control of Scer\GAL4^Act5C.PU in flw^FP41 mutant follicle cell clones results in overproliferation in anterior and lateral follicle cells. The amount of cell overproliferation in posterior follicle cells is increased compared to flw^FP41 clones alone.

(Sun et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

flw^FP41 is rescued by flw^UAS.Tag:HA/Scer\GAL4^e22c

(Sun et al., 2011)

Not rescued by

flw^FP41 is not rescued by Scer\GAL4^{VP16.mat.αTub67C}/flw^UAS.cRa

(Yamamoto et al., 2013)

Comments

Expression of flw^Scer\UAS.cRa under the control of Scer\GAL4^tub.PU does not rescue the lethality of flw^FP41.

(Yamamoto et al., 2013)

Expression of flw^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^e22c rescues the oocyte polarity defects seen when the posterior follicle cells are mutant for flw^FP41.

(Sun et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51338

y¹ w^* flw^FP41 g² f¹ P{neoFRT}19A/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

flw^FP41

(Moreira et al., 2019, Yamamoto et al., 2013, Sun et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (3)

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