FB2024_03 , released June 25, 2024
Allele: Scer\GAL80FRT.αTub84B
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General Information
Symbol
Scer\GAL80FRT.αTub84B
Species
S. cerevisiae
Name
FlyBase ID
FBal0267931
Feature type
allele
Associated gene
Scer\GAL80
Associated Insertion(s)
Carried in Construct
P{tubP(FRT.stop)GAL80}
Also Known As
tub>stop>GAL80, Tub-FRT-stop-FRT-GAL80, tubP>stop>GAL80
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Bohm et al., 2010)
Progenitor genotype
Carried in construct
P{tubP(FRT.stop)GAL80}
Cytology
Description

A FRT cassette containing stop sequences separates a αTub84B promoter from the GAL80 coding region.

(Bohm et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
GAL80
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL80Scer\FRT.αTub84B has no effect on the wing expansion defect caused by expression of GlΔ.Scer\UAS under the control of Scer\GAL4Ccap.PP. When FLPase is used to remove the Scer\FRT cassette in Scer\GAL80Scer\FRT.αTub84B, generating Scer\GAL80αTub84B.PB which expresses Scer\GAL80, the failure of wing expansion caused by expression of GlΔ.Scer\UAS under the control of Scer\GAL4Ccap.PP is completely suppressed.

      (Bohm et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Scer\GAL80Scer\FRT.αTub84B has no effect on the eye degeneration and depigmentation caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF. When FLPase is used to remove the Scer\FRT cassette in Scer\GAL80Scer\FRT.αTub84B, generating Scer\GAL80αTub84B.PB which expresses Scer\GAL80, the eye phenotype caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is completely suppressed.

      Flies carrying Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF and Scer\GAL80αTub84B.PG have normal eyes, due to repression of Scer\GAL4GMR.PF expression by Scer\GAL80αTub84B.PG. This repression can be removed using FLPase (which removes the Scer\FRT cassette containing the Scer\GAL80 coding region from Scer\GAL80αTub84B.PG).

      (Bohm et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL80FRT.αTub84B
      Scer\GAL80Scer\FRT.αTub84B
      Name Synonyms
      Secondary FlyBase IDs
        References (19)