FB2024_03 , released June 25, 2024
Allele: Dmel\E(spl)m8-HLHSD.Δgro.UAS
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General Information
Symbol
Dmel\E(spl)m8-HLHSD.Δgro.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0282767
Feature type
allele
Associated gene
Dmel\E(spl)m8-HLH
Associated Insertion(s)
Carried in Construct
P{UAS-E(spl)m8-HLH.SD.ΔGro}
Key Links
Transgenic product class
missense_variant
(Kahali et al., 2009)
polypeptide_post_translational_processing_variant
(Kahali et al., 2009)
stop_gained
(Kahali et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kahali et al., 2009)
polypeptide_post_translational_processing_variant
(Kahali et al., 2009)
stop_gained
(Kahali et al., 2009)
Mutagen
in vitro construct
(Kahali et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-E(spl)m8-HLH.SD.ΔGro}
Cytology
Description

UAS drives expression of E(spl)m8-HLH in which a STOP codon is substituted in place of the W176 of the WRPW motif, thus removing this motif and abolishing gro protein binding. In addition, S159 is changed to D to mimic phosphorylation.

(Kahali et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
E(spl)m8-HLH
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS with Scer\GAL4sca-537.4 has no effect on the eye or interommatidial bristles, but results in significantly more flies with ectopic scutellar macrochaetes than controls.

      Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS with Scer\GAL4455.2 results in ectopic scutellar macrochaetes.

      Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS with Scer\GAL4h-H10 is ineffective.

      (Kahali et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      NOT Enhancer of
      Statement
      Reference

      E(spl)m8-HLHSD.Δgro.UAS/Scer\GAL4GMR.PU is a non-enhancer of eye phenotype of Nspl-1

      (Kahali et al., 2009)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      E(spl)m8-HLHSD.Δgro.UAS/Scer\GAL4GMR.PU is a non-suppressor of eye phenotype of Nspl-1

      (Kahali et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Df(3R)Espl22/+ modestly enhances the ectopic scutellar bristle phenotype of E(spl)m8-HLHSD.Δgro.Scer\UAS Scer\GAL4455.2.

      Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS mediated by Scer\GAL4h-H10 suppresses the Nspl-1/Y rough and reduced eye and appears to restore ommatidial phasing.

      Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS mediated by Scer\GAL4sca-109-68 suppresses the Nspl-1/Y rough and reduced eye.

      Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS mediated by Scer\GAL4h-H10 potently suppresses the Nspl-1/Y scutellar bristle phenotypes.

      (Kahali et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4455.2/E(spl)m8-HLHSD.Δgro.UAS rescues E(spl)m8-HLHUAS.cUa

      (Kahali et al., 2009)
      Comments

      Co-expression of E(spl)m8-HLHSD.Δgro.Scer\UAS fully attenuates the potent loss of scutellar bristles seen in E(spl)m8-HLHScer\UAS.cUa Scer\GAL4455.2 flies.

      (Kahali et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      E(spl)m8-HLHSD.Δgro.Scer\UAS
      E(spl)m8-HLHSD.Δgro.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)