FlyBase Allele Report: Dmel\crb[intra.UAS.Tag:MYC]

General Information

Symbol

Dmel\crb^{intra.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0286307

Feature type

allele

Associated gene

Dmel\crb

Associated Insertion(s)

Carried in Construct

P{UAS-crb.intra.myc}

Also Known As

UAS-crb^intra, Crb^intra, UAS-crb^Myc-intra, UAS-crb^intramyc

Key Links

Transgenic product class

inframe_deletion

(Wodarz et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Wodarz et al., 1995)

Mutagen

in vitro construct

(Wodarz et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-crb.intra.myc}

Cytology

Description

UASt regulatory sequences drive expression of crb cDNA lacking the complete extracellular domain except the N terminal region including the signal peptide, and tagged with a Tag:MYC epitope.

(Wodarz et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

crb

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

amnioserosa, with Scer\GAL4^332.3

(Wodarz et al., 1995)

amnioserosa, with Scer\GAL4^ptc-559.1

(Wodarz et al., 1995)

embryo, with Scer\GAL4^{VP16.mat.αTub67C}

(Hong et al., 2001)

embryo | embryonic stage 16, with Scer\GAL4^ct.PB

(Campbell et al., 2009)

embryonic epidermis, with Scer\GAL4^da.G32

(Klebes and Knust, 2000)

embryonic Malpighian tubule, with Scer\GAL4^ct.PB

(Campbell et al., 2009)

epidermis, with Scer\GAL4^da.G32

(Wodarz et al., 1995)

epidermis, with Scer\GAL4^ptc-559.1

(Wodarz et al., 1995)

eye, with Scer\GAL4^GMR.PF

(Mok and Choi, 2022, Chen et al., 2009, Liu et al., 2009, Izaddoost et al., 2002)

eye, with Scer\GAL4^GMR.PU

(Yeom et al., 2015, Steffensmeier et al., 2013)

ommatidium | third instar larval stage, with Scer\GAL4^GMR.PF

(Fan et al., 2003)

photoreceptor & adherens junction, with Scer\GAL4^GMR.PF

(Izaddoost et al., 2002)

photoreceptor | third instar larval stage, with Scer\GAL4^GMR.PF

(Fan et al., 2003)

photoreceptor cell R1 | third instar larval stage, with Scer\GAL4^GMR.PF

(Fan et al., 2003)

photoreceptor cell R6 | third instar larval stage, with Scer\GAL4^GMR.PF

(Fan et al., 2003)

tendon cell, with Scer\GAL4^da.G32

(Wodarz et al., 1995)

ventral denticle belt, with Scer\GAL4^ptc-559.1

(Wodarz et al., 1995)

wing, with Scer\GAL4^en.PU

(Zhang et al., 2015)

wing disc, with Scer\GAL4^Bx-MS1096

(Yeom et al., 2015)

Detailed Description

Statement

Reference

Flies expressing crb^{intra.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU have a rough eye phenotype.

Expression of crb^{intra.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in dramatic overgrowth of the wing disc.

(Yeom et al., 2015)

Expression of crb^{intra.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en.PU leads to enlarged adult wings.

(Zhang et al., 2015)

Expression of crb^{intra.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU results in a scab in place of the eye in the adult head cuticle.

(Steffensmeier et al., 2013)

Overexpression of crb^{intra.Scer\UAS.T:Hsap\MYC} in the renal tubules, under the control of Scer\GAL4^ct.PB does not affect tubule cell morphology in stage 11 to 13 embryos. Expression in stage 16 tubules results in a loss of junctional integrity where tubule cell polarity is compromised and tubules become multilayered cords of cells lacking a clear lumen.

(Campbell et al., 2009)

Expression of crb^{intra.Scer\UAS.T:Hsap\MYC} using Scer\GAL4^GMR.PF causes severe external and internal eye phenotypes.

(Liu et al., 2009)

Expression of crb^{intra.Scer\UAS.T:Hsap\MYC} using Scer\GAL4^GMR.PF disrupts the regular array of ommatidial lattices and slightly reduces the number of elav-and B-positive cells.

(Fan et al., 2003)

When expression is driven in the eye by Scer\GAL4^GMR.PF causes a rough eye phenotype. 97% of the photoreceptors in the pupal eye contain multiple adherens junctions of variable length. Polarity of photoreceptor is disrupted.

(Izaddoost et al., 2002)

When crb^{intra.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} in the embryo, cell polarity is disrupted.

(Hong et al., 2001)

Over expression of crb^{intra.Scer\UAS.T:Hsap\MYC} when driven by Scer\GAL4^da.G32 produces embryos with an epidermis that develops as a multilayered tissue (unlike the single layered epithelium seen in wild-type embryos). The cells also show defective polarity characteristics.

(Klebes and Knust, 2000)

Phenotype is exactly as for crb^Scer\UAS.cWa, when driven by the same Scer\GAL4 lines.

(Wodarz et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has visible phenotype, enhanceable by α-Spec^rg41/alpha-Spec[+]

(Chen et al., 2009)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has visible phenotype, enhanceable by beta-Spec[+]/β-Spec^G113

(Chen et al., 2009)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has visible phenotype, enhanceable by kst[+]/kst¹

(Chen et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has visible | adult stage phenotype, suppressible by Mnat9^KK109382, Scer\GAL4^GMR.PF

(Mok and Choi, 2022)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has visible | adult stage phenotype, suppressible by Mnat9^GD17314, Scer\GAL4^GMR.PF

(Mok and Choi, 2022)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has visible | adult stage phenotype, suppressible by Mnat9^GD7326, Scer\GAL4^GMR.PF

(Mok and Choi, 2022)

Scer\GAL4^Bx-MS1096, crb^{intra.UAS.Tag:MYC} has hyperplasia phenotype, suppressible by galla-1^NIG.30152R/Scer\GAL4^Bx-MS1096

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has visible phenotype, suppressible | partially by galla-1^Δ182/galla-1^Δ182

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has visible phenotype, suppressible | partially by galla-1^Δ182/galla-1[+]

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has visible phenotype, suppressible by Xpd^HMJ21396/Scer\GAL4^GMR.PU

(Yeom et al., 2015)

Scer\GAL4^Bx-MS1096, crb^{intra.UAS.Tag:MYC} has hyperplasia phenotype, suppressible by Xpd^HMJ21396/Scer\GAL4^Bx-MS1096

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has visible phenotype, suppressible | partially by Xpd^SH2137/Xpd[+]

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has visible phenotype, suppressible | partially by galla-2^Δ203/galla-2[+]

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has visible phenotype, suppressible by galla-1^NIG.30152R/Scer\GAL4^GMR.PU

(Yeom et al., 2015)

Scer\GAL4^en.PU, crb^{intra.UAS.Tag:MYC} has abnormal size phenotype, suppressible by tai[+]/tai^k15101

(Zhang et al., 2015)

Scer\GAL4^en.PU, crb^{intra.UAS.Tag:MYC} has abnormal size phenotype, suppressible by tai[+]/tai^k05809

(Zhang et al., 2015)

Scer\GAL4^en.PU, crb^{intra.UAS.Tag:MYC} has abnormal size phenotype, suppressible by tai[+]/tai^61G1

(Zhang et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has eye phenotype, enhanceable by α-Spec^rg41/alpha-Spec[+]

(Chen et al., 2009)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has eye phenotype, enhanceable by beta-Spec[+]/β-Spec^G113

(Chen et al., 2009)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has eye phenotype, enhanceable by kst[+]/kst¹

(Chen et al., 2009)

NOT Enhanced by

Statement

Reference

Scer\GAL4^da.G32, crb^{intra.UAS.Tag:MYC} has embryonic epidermis phenotype, non-enhanceable by stg^unspecified

(Klebes and Knust, 2000)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible by Mnat9^KK109382, Scer\GAL4^GMR.PF

(Mok and Choi, 2022)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible by Mnat9^GD17314, Scer\GAL4^GMR.PF

(Mok and Choi, 2022)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible by Mnat9^GD7326, Scer\GAL4^GMR.PF

(Mok and Choi, 2022)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible by galla-1^NIG.30152R/Scer\GAL4^GMR.PU

(Yeom et al., 2015)

Scer\GAL4^Bx-MS1096, crb^{intra.UAS.Tag:MYC} has wing disc phenotype, suppressible by galla-1^NIG.30152R/Scer\GAL4^Bx-MS1096

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible | partially by galla-1^Δ182/galla-1^Δ182

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible | partially by galla-1^Δ182/galla-1[+]

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible by Xpd^HMJ21396/Scer\GAL4^GMR.PU

(Yeom et al., 2015)

Scer\GAL4^Bx-MS1096, crb^{intra.UAS.Tag:MYC} has wing disc phenotype, suppressible by Xpd^HMJ21396/Scer\GAL4^Bx-MS1096

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible | partially by Xpd^SH2137/Xpd[+]

(Yeom et al., 2015)

Scer\GAL4^GMR.PU, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible | partially by galla-2^Δ203/galla-2[+]

(Yeom et al., 2015)

Scer\GAL4^en.PU, crb^{intra.UAS.Tag:MYC} has wing phenotype, suppressible by tai[+]/tai^k15101

(Zhang et al., 2015)

Scer\GAL4^en.PU, crb^{intra.UAS.Tag:MYC} has wing phenotype, suppressible by tai[+]/tai^k05809

(Zhang et al., 2015)

Scer\GAL4^en.PU, crb^{intra.UAS.Tag:MYC} has wing phenotype, suppressible by tai[+]/tai^61G1

(Zhang et al., 2015)

Scer\GAL4^GMR.PF, crb^{intra.UAS.Tag:MYC} has eye phenotype, suppressible by Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Liu et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.G32, crb^{intra.UAS.Tag:MYC} has embryonic epidermis phenotype, non-suppressible by stg^unspecified

(Klebes and Knust, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of galla-1^NIG.30152R strongly suppresses the rough eye phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of galla-1^NIG.30152R strongly suppresses the wing disc overproliferation phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^Bx-MS1096.

One copy of galla-1^Δ182 does not obviously suppress the small and rough eye phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PU. Homozygous galla-1^Δ182 results in significant rescue of the eye phenotype.

Expression of Xpd^HMJ21396 strongly suppresses the rough eye phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Xpd^HMJ21396 strongly suppresses the wing disc overproliferation phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^Bx-MS1096.

One copy of Xpd^SH2137 strongly suppresses the small and rough eye phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PU.

One copy of galla-2^Δ203 considerably suppresses the small and rough eye phenotype seen when crb^{intra.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PU.

(Yeom et al., 2015)

The presence of tai^k15101/+, tai^k05809/+ or tai^61G1/+ suppresses the enlarged wings of adult females expressing crb^{intra.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en.PU.

(Zhang et al., 2015)

Co-expression of Scer\GAL4^GMR.PF mitigates the external and internal eye phenotypes seen in crb^{intra.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF flies.

(Liu et al., 2009)

The addition of stg^unspecified has no effect on the phenotype of embryos expressing crb^{intra.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^da.G32.

(Klebes and Knust, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^FRT.Act5C/crb^{intra.UAS.Tag:MYC} rescues crb^11A22

(Li et al., 2008)

Scer\GAL4^unspecified/crb^{intra.UAS.Tag:MYC} rescues crb[-]

(Izaddoost et al., 2002)

Scer\GAL4^unspecified/crb^{intra.UAS.Tag:MYC} rescues crb^11A22

(Wodarz et al., 1995)

Partially rescues

Scer\GAL4^385.3/crb^{intra.UAS.Tag:MYC} partially rescues crb^unspecified

(Klebes and Knust, 2000)

Comments

The addition of crb^{intra.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^385.3 partially restores the loss of the zonula adherens seen in crb^unspecified embryos. The embryonic cuticle phenotype is also partially restored.

(Klebes and Knust, 2000)

Rescues crb² as does crb^Scer\UAS.cWa, restoring an almost wild type cuticle pattern including denticle belts.

(Wodarz et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Crb^intra

(Chen et al., 2009)

crb^ICD

(Hafezi et al., 2012)

crb^Myc-intra

(Izaddoost et al., 2002)

crb^{Scer\UAS.intra.T:Hsap\MYC}

crb^intra-myc

(Wodarz et al., 1995)

crb^{intra.Scer\UAS.T:Hsap\MYC}

crb^{intra.UAS.T:Myc}

crb^{intra.UAS.Tag:MYC}

crb^intra

(Zhang et al., 2015)

crumbs^myc-intra

(Medina et al., 2002)

Name Synonyms

Secondary FlyBase IDs

FBal0042950
FBal0246426

References (18)

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