FlyBase Allele Report: Dmel\erm[UAS.ORF.GW.Tag:HA]

UAS regulatory sequences drive expression of a full-length erm open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Also carries

recombinase target site

FRT5

recombinase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | embryonic stage, with Scer\GAL4^elav-C155

(Curt et al., 2019)

lethal | male limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

visible | female limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Phenotype Manifest In

ganglion mother cell | embryonic stage, with Scer\GAL4^elav-C155

(Curt et al., 2019)

larval ventral nerve cord | embryonic stage, with Scer\GAL4^elav-C155

(Curt et al., 2019)

neuroblast | embryonic stage, with Scer\GAL4^elav-C155

(Curt et al., 2019)

wing | female limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Detailed Description

Statement

Reference

Expression of erm^{UAS.ORF.GW.Tag:HA} under the control of Scer\GAL4^elav-C155 increases mitotic proliferation of neuroblasts and their daughter cells in the ventral nerve cord during late embryogenesis, compared to controls; expression under the control of Scer\GAL4^vg.int2.1 in the larval wing disc has only a limited effect on the number of neuroblasts and their daughter cells, compared to controls.

(Curt et al., 2019)

Expression of erm^{Scer\UAS.ORF.GW.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 results in female flies with severe wing size and patterning defects.

(Schertel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA} has increased occurrence of cell division | embryonic stage phenotype, enhanceable by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}/tll^UAS.cKa, Scer\GAL4^elav-C155

(Curt et al., 2019)

Enhancer of

Statement

Reference

otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of increased occurrence of cell division | embryonic stage phenotype of Doc2^UAS.cRa, Scer\GAL4^elav-C155

(Curt et al., 2019)

Doc2^UAS.cRa, otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^elav-C155 is an enhancer of increased occurrence of cell division | embryonic stage phenotype of Scer\GAL4^elav-C155, tll^UAS.cKa

(Curt et al., 2019)

Doc2^UAS.cRa, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of increased occurrence of cell division | embryonic stage phenotype of Scer\GAL4^elav-C155, otp^{UAS.ORF.GW.Tag:HA}

(Curt et al., 2019)

Other

Statement

Reference

Doc2^UAS.cRa, Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased cell number | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased occurrence of cell division | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has abnormal cell number | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased occurrence of cell division | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased cell number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased occurrence of cell division | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased occurrence of cell division | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased cell number | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased cell number | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has increased occurrence of cell division | embryonic stage phenotype

(Curt et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA} has neuroblast | embryonic stage phenotype, enhanceable by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}/tll^UAS.cKa, Scer\GAL4^elav-C155

(Curt et al., 2019)

Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA} has ganglion mother cell | embryonic stage phenotype, enhanceable by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}/tll^UAS.cKa, Scer\GAL4^elav-C155

(Curt et al., 2019)

Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA} has larval ventral nerve cord | embryonic stage phenotype, enhanceable by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}/tll^UAS.cKa, Scer\GAL4^elav-C155

(Curt et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^vg.int2.1, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has wing disc phenotype, suppressible | partially by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^vg.int2.1

(Curt et al., 2019)

Scer\GAL4^vg.int2.1, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | ectopic | larval stage phenotype, suppressible | partially by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^vg.int2.1

(Curt et al., 2019)

Scer\GAL4^vg.int2.1, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has ganglion mother cell | ectopic | larval stage phenotype, suppressible | partially by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^vg.int2.1

(Curt et al., 2019)

Scer\GAL4^vg.int2.1, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval neuron | ectopic | larval stage phenotype, suppressible | partially by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^vg.int2.1

(Curt et al., 2019)

Scer\GAL4^vg.int2.1, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval glial cell | ectopic | larval stage phenotype, suppressible | partially by Doc2^UAS.cRa/otp^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^vg.int2.1

(Curt et al., 2019)

Enhancer of

Statement

Reference

Doc2^UAS.cRa, otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4^elav-C155, tll^UAS.cKa

(Curt et al., 2019)

Doc2^UAS.cRa, otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4^elav-C155, tll^UAS.cKa

(Curt et al., 2019)

otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Doc2^UAS.cRa, Scer\GAL4^elav-C155

(Curt et al., 2019)

otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Doc2^UAS.cRa, Scer\GAL4^elav-C155

(Curt et al., 2019)

otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Doc2^UAS.cRa, Scer\GAL4^elav-C155

(Curt et al., 2019)

erm^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^vg.int2.1 is an enhancer of wing disc phenotype of Scer\GAL4^vg.int2.1, tll^UAS.cKa

(Curt et al., 2019)

erm^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^vg.int2.1 is an enhancer of neuroblast | larval stage | ectopic phenotype of Scer\GAL4^vg.int2.1, tll^UAS.cKa

(Curt et al., 2019)

erm^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^vg.int2.1 is an enhancer of ganglion mother cell | larval stage | ectopic phenotype of Scer\GAL4^vg.int2.1, tll^UAS.cKa

(Curt et al., 2019)

Doc2^UAS.cRa, otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4^elav-C155, tll^UAS.cKa

(Curt et al., 2019)

Doc2^UAS.cRa, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4^elav-C155, otp^{UAS.ORF.GW.Tag:HA}

(Curt et al., 2019)

Doc2^UAS.cRa, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4^elav-C155, otp^{UAS.ORF.GW.Tag:HA}

(Curt et al., 2019)

Doc2^UAS.cRa, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa, Scer\GAL4^elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4^elav-C155, otp^{UAS.ORF.GW.Tag:HA}

(Curt et al., 2019)

Other

Statement

Reference

Doc2^UAS.cRa, Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has ganglion mother cell | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has ganglion mother cell | increased number | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval glial cell | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | increased number | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval neuron | increased number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | increased number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^elav-C155, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | increased number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has ganglion mother cell | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has ganglion mother cell | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has ganglion mother cell | increased number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | increased number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval neuron | increased number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^da.PU, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval glial cell | increased number | embryonic stage phenotype

(Curt et al., 2019)

Scer\GAL4^vg.int2.1, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval neuron | ectopic | larval stage phenotype

(Curt et al., 2019)

Scer\GAL4^vg.int2.1, erm^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has embryonic/larval glial cell | ectopic | larval stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^wg.PU, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has ganglion mother cell | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has larval ventral nerve cord | embryonic stage phenotype

(Curt et al., 2019)

Doc2^UAS.cRa, Scer\GAL4^pros.PMG, erm^{UAS.ORF.GW.Tag:HA}, otp^{UAS.ORF.GW.Tag:HA}, tll^UAS.cKa has neuroblast | embryonic stage phenotype

(Curt et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of tll^UAS.cKa, otp^{UAS.ORF.GW.Tag:HA}, erm^{UAS.ORF.GW.Tag:HA} and Doc2^UAS.cRa under the control of Scer\GAL4^elav-C155 significantly increases total cell number and the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4^wg.PU results in a trend towards increased symmetric (abnormal) neuroblast division and significantly increased asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4^pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4^da.PU increases the number of mitotic cells, neuroblasts, daughters and neurons, and reduces the number of glia, with a thickening of the CNS, compared to controls.

Expression of tll^UAS.cKa and erm^{UAS.ORF.GW.Tag:HA} under the control of Scer\GAL4^elav-C155 significantly increases the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4^wg.PU results in a significant increase in symmetric (abnormal) neuroblast division and asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4^pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4^da.PU increases the number of mitotic cells, neuroblasts, neuroblast daughters, neurons and glia, compared to controls; expression under the control of Scer\GAL4[vg.int2.1] results in the formation of neurons and glia in the larval wing disc, these are not seen in controls.

(Curt et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

83308