FB2024_03 , released June 25, 2024
Allele: Dmel\Doc2UAS.cRa
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General Information
Symbol
Dmel\Doc2UAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Reim
FlyBase ID
FBal0150885
Feature type
allele
Associated gene
Dmel\Doc2
Associated Insertion(s)
Carried in Construct
P{UAS-Doc2.R}
Also Known As
UAS-Doc2
Key Links
Transgenic product class
cDNA
(Reim et al., 2003)
wild_type
(Reim et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Reim et al., 2003)
wild_type
(Reim et al., 2003)
Mutagen
in vitro construct
(Reim et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-Doc2.R}
Cytology
Description

UASt regulatory sequences drive expression of the Doc2 c6.2 cDNA.

(Reim et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Doc2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Doc2UAS.cRa under the control of Scer\GAL4elav-C155 increases mitotic proliferation of neuroblasts and their daughter cells in the ventral nerve cord during late embryogenesis, compared to controls; expression under the control of Scer\GAL4vg.int2.1 in the larval wing disc has only a limited effect on the number of neuroblasts and their daughter cells, compared to controls.

      (Curt et al., 2019)

      Expression of Doc2Scer\UAS.cRa in the dorsal mesoderm in stage 11-12 embryos, driven by Scer\GAL4tin.cBa, results in the formation of additional cardioblasts, but a reduction in the number of odd-positive pericardial cells. However, eve-positive pericardial cells and dorsal muscle are less affected. The nuclei of the somatic mesoderm in dorsolateral areas are arranged irregularly. Expression of Doc2Scer\UAS.cRa throughout the mesoderm of the embryo, driven by Scer\GAL4twi.2PE, results in the formation of extra cardioblasts, an effect that is stronger in dorsal areas of the mesoderm.

      (Reim and Frasch, 2005)

      Expression of Doc2Scer\UAS.cRa under the control of Scer\GAL4dpp.blk1 results in wings which lack distal structures and have broadened wing veins. Antennae lack aristae and legs are shortened.

      (Reim et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      Other
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Statement
      Reference

      Doc2UAS.cRa, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4vg.int2.1 is an enhancer of wing disc phenotype of Scer\GAL4vg.int2.1, tllUAS.cKa

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4vg.int2.1 is an enhancer of neuroblast | larval stage | ectopic phenotype of Scer\GAL4vg.int2.1, tllUAS.cKa

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4vg.int2.1 is an enhancer of ganglion mother cell | larval stage | ectopic phenotype of Scer\GAL4vg.int2.1, tllUAS.cKa

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4elav-C155, tllUAS.cKa

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4elav-C155, tllUAS.cKa

      (Curt et al., 2019)

      Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4elav-C155, tllUAS.cKa

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4tin.cBa is an enhancer of cardioblast | increased number phenotype of Scer\GAL4tin.cBa, pnrUAS.cHa

      (Reim and Frasch, 2005)

      tinUAS.cYa/Doc2UAS.cRa, Scer\GAL4tin.cBa is an enhancer of cardioblast | increased number phenotype of Scer\GAL4tin.cBa, pnrUAS.cHa

      (Reim and Frasch, 2005)
      Suppressor of
      Other
      Statement
      Reference

      Doc2UAS.cRa, Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval glial cell | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has embryonic/larval neuron | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | increased number | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has ganglion mother cell | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has larval ventral nerve cord | embryonic stage phenotype

      (Curt et al., 2019)

      Doc2UAS.cRa, Scer\GAL4pros.PMG, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | embryonic stage phenotype

      (Curt et al., 2019)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of tllUAS.cKa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA and Doc2UAS.cRa under the control of Scer\GAL4elav-C155 significantly increases total cell number and the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4wg.PU results in a trend towards increased symmetric (abnormal) neuroblast division and significantly increased asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4da.PU increases the number of mitotic cells, neuroblasts, daughters and neurons, and reduces the number of glia, with a thickening of the CNS, compared to controls.

      (Curt et al., 2019)

      Expression of Doc2Scer\UAS.cRa, driven by Scer\GAL4tin.cBa, results in efficient rescue of cardioblast specification in a Df(3L)DocA mutant background. When Doc2Scer\UAS.cRa is coexpressed with either tinScer\UAS.cYa or pnrScer\UAS.cHa, or both transgenes together, large numbers of extra cardioblasts are formed. Expression of all three transgenes results in double the amount of cardioblasts compared to wild type. Coexpression of Doc2Scer\UAS.cRa, tinScer\UAS.cYa, and pnrScer\UAS.cHa, under the control of Scer\GAL4twi.2PE, causes widespread ectopic cardioblast formation to a much greater extent than expression of Doc2Scer\UAS.cRa alone or in combination with tinScer\UAS.cYa plus pnrScer\UAS.cHa.

      (Reim and Frasch, 2005)

      Expression of Doc2Scer\UAS.cRa under the control of Scer\GAL4c381 rescues the germ band retraction defects of Df(3L)DocA embryos.

      (Reim et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Doc2Scer\UAS.cRa
      Doc2UAS.cRa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Reim
      (Reim et al., 2003)
      Secondary FlyBase IDs
        References (6)