Expression of pnrUAS.cHa under the control of Scer\GAL4He.PZ or Scer\GAL4lz-gal4 leads to a decrease in larval crystal cell numbers when compared to controls.
Expression of pnrScer\UAS.cHa under the control of Scer\GAL4C-765 leads to the formation of ectopic sensory organ precursors.
Expression of pnrScer\UAS.cHa under the control of Scer\GAL4Bx-MS1096 leads to excess dorsocentral bristles.
Overexpression of pnrScer\UAS.cHa in the mesoderm of embryos, driven by both Scer\GAL4how-24B and Scer\GAL4twi.PG, induces the formation of extra cardioblasts.
Expression of pnrScer\UAS.cHa in the dorsal mesoderm in stage 11-12 embryos, driven by Scer\GAL4tin.cBa, results in the production of a few extra cardioblasts.
Ectopic expression of pnrScer\UAS.cHa in the lateral domain of the cardiogenic mesoderm, driven by either Scer\GAL4Mef2.PR or Scer\GAL4twi.PG, causes a marked reduction of lymph-gland progenitors and pericardial cells in stage 16 embryos.
Flies expressing pnrScer\UAS.cHa under the control of Scer\GAL4pnr-MD237 are virtually wild type. The entire epidermis becomes dorsalised in embryos expressing pnrScer\UAS.cHa under the control of Scer\GAL4arm.PS. The abdominal segments have a continuous belt of dorsal triangles around their circumference, indicating that the transformation is towards the mediodorsal pattern. Expression of pnrScer\UAS.cHa under the control of Scer\GAL4Ubx.PdC results in transformation of the ventral epidermis into dorsal epidermis; the ventral denticles of most of the abdomen are replaced by dorsal spinules. Transformation from ventral to dorsal structures is also seen in segment T3 and the posterior part of T2 (the anterior part of T2 is the same as wild type). The ventral nerve cord is affected. The amnioserosa develops normally and germband retraction is normal. Expression of pnrScer\UAS.cHa under the control of Scer\GAL4en-e16E results in transformation of ventral to dorsal epidermis in the P compartments. Expression of pnrScer\UAS.cHa under the control of Scer\GAL4wg-MD758 results in transformation of ventral to dorsal epidermis in a portion of the anterior compartment of segments. The amnioserosa develops normally and germband retraction is normal in embryos expressing pnrScer\UAS.cHa under the control of Scer\GAL4LP1.
When expression of pnrScer\UAS.cHa is driven in clones by Scer\GAL4Ubx.PdC in the lateral (LAT) region of the notum, they are eliminated or grow abnormally, in the medial (MED) region they grow almost normally. In clones that overlapped the regions, the cells situated in the MED region differentiated normally, in the LAT region, they tend to sort out from surrounding cells to form vesicles with sharp boundaries. pnrScer\UAS.cHa when driven by Scer\GAL4pnr-MD237 produces no defects except supernumerary bristles in the scutellum. When driven by Scer\GAL4ost-em462, there is an enlargement of the MED region as indicated by the increased number of dorsocentral bristles. When driven by Scer\GAL4ap-md544, only structures that correspond to the MED region are formed, the LAT region does not develop. The MED pattern is normal except for an increase in the number of dorsocentral bristles and a higher density of microchaetae in the dorsocentral region. When expression of pnrScer\UAS.cHa is driven in clones by Scer\GAL4Ubx.PdC in the wing, the clones develop structures typical of the notum with the characteristic trichome density of the notum as well as notal bristles. Notal structures are formed in a cell-autonomous manner. These wing to notum transformations appear anywhere in the wing, except the distal end, but are found preferentially in the more proximal regions. Clones induced during the larval first instar can build large portion of the notum pattern. Clones differentiating notal structures appear exclusively in the anterior compartment. In the posterior compartment clones differentiate patches of pigmented cuticle devoid of bristles interpreted as being postnotum. Expression of pnrScer\UAS.cHa by Scer\GAL4ap-md544, Scer\GAL4nub-AC-62, Scer\GAL4Bx-MS1096, Scer\GAL4C-765, Scer\GAL4bi-omb-Gal4, or Scer\GAL4vg.quadrant produce wing to notum transformations. Expression of pnrScer\UAS.cHa when driven by Scer\GAL4MD743 leads top sternites that acquire pigmentation and bristle patterns similar to those of the tergites. In some cases the entire set of sternites are transformed into tergites, resulting in flies with an almost complete dorsoventral transformation of the abdomen.
When pnrScer\UAS.cHa is driven by Scer\GAL4pnr-MD237, an excess of dorsocentral bristles is seen.
Embryos expressing pnrScer\UAS.cHa under the control of Scer\GAL4twi.PB have an enlarged heart, due to the overspecification of cardial cells (both within the heart tube and throughout the dorsal region of the embryo). There is a decrease in the number of eve-expressing pericardial cells, and a decrease and abnormal organisation of dorsal body wall muscles. There is an apparent loss of cells within the visceral mesoderm lineage.
When expression is driven by Scer\GAL4C-765, many macrochaetae develop in the region lateral to the usual dorsocentral (DC) area bristles.
Overexpression of pnrScer\UAS.cHa using Scer\GAL4pnr-MD237 leads to an excess of dorsocentral bristles.
Scer\GAL4pnr-MD237, pnrUAS.cHa has visible phenotype, enhanceable by Df(2L)ushrev18
Scer\GAL4He.PZ, pnrUAS.cHa has decreased cell number | wandering third instar larval stage phenotype, non-enhanceable by BxUAS.Tag:HA, Scer\GAL4He.PZ
Scer\GAL4He.PZ, pnrUAS.cHa has decreased cell number | wandering third instar larval stage phenotype, non-suppressible by BxUAS.Tag:HA, Scer\GAL4He.PZ
pnrUAS.cHa/Scer\GAL4pnr-MD237 is a suppressor of visible | adult stage phenotype of Scer\GAL4pnr-MD237, tkvDN.UAS.cUa
pnrUAS.cHa, Scer\GAL4He.PZ is a suppressor of increased cell number | wandering third instar larval stage phenotype of BxUAS.Tag:HA, Scer\GAL4He.PZ
Scer\GAL4pnr-MD237, pnrUAS.cHa/pnrMD237, ppoEP2478 has visible phenotype
Scer\GAL4pnr-MD237, pnrMD237, pnrUAS.cHa, ppoEP2478 has visible phenotype
Scer\GAL4tin.cBa, pnrUAS.cHa has cardioblast | increased number phenotype, enhanceable by Doc2UAS.cRa, Scer\GAL4tin.cBa
Scer\GAL4tin.cBa, pnrUAS.cHa has cardioblast | increased number phenotype, enhanceable by tinUAS.cYa/Doc2UAS.cRa, Scer\GAL4tin.cBa
Scer\GAL4tin.cBa, pnrUAS.cHa has cardioblast | increased number phenotype, enhanceable by Scer\GAL4tin.cBa/tinUAS.cYa
Scer\GAL4pnr-MD237, pnrUAS.cHa has dorsocentral bristle phenotype, enhanceable by Df(2L)ushrev18
Scer\GAL4He.PZ, pnrUAS.cHa has embryonic/larval crystal cell | wandering third instar larval stage phenotype, non-enhanceable by BxUAS.Tag:HA, Scer\GAL4He.PZ
Scer\GAL4He.PZ, pnrUAS.cHa has embryonic/larval crystal cell | wandering third instar larval stage phenotype, non-suppressible by BxUAS.Tag:HA, Scer\GAL4He.PZ
pnrUAS.cHa, Scer\GAL4tin.cBa is an enhancer of cardioblast | increased number phenotype of Doc2UAS.cRa, Scer\GAL4tin.cBa
pnrUAS.cHa/tinUAS.cYa, Scer\GAL4twi.2PE is an enhancer of cardioblast | increased number phenotype of Doc2UAS.cRa, Scer\GAL4twi.2PE
pnrUAS.cHa/tinUAS.cYa, Scer\GAL4tin.cBa is an enhancer of cardioblast | increased number phenotype of Doc2UAS.cRa, Scer\GAL4tin.cBa
pnrUAS.cHa/Scer\GAL4pnr-MD237 is a suppressor of adult thorax phenotype of Scer\GAL4pnr-MD237, tkvDN.UAS.cUa
pnrUAS.cHa, Scer\GAL4He.PZ is a suppressor of embryonic/larval crystal cell | increased number | wandering third instar larval stage phenotype of BxUAS.Tag:HA, Scer\GAL4He.PZ
pnrUAS.cHa/Scer\GAL4pnr-MD237 is a suppressor of thorax phenotype of ChiE
pnrUAS.cHa/Scer\GAL4pnr-MD237 is a non-suppressor of dorsocentral bristle phenotype of ChiE
Scer\GAL4pnr-MD237, pnrUAS.cHa/pnrMD237, ppoEP2478 has scutellum phenotype
Scer\GAL4pnr-MD237, pnrUAS.cHa/pnrMD237, ppoEP2478 has chaeta phenotype
Scer\GAL4pnr-MD237, pnrMD237, pnrUAS.cHa, ppoEP2478 has scutellum phenotype
Scer\GAL4pnr-MD237, pnrMD237, pnrUAS.cHa, ppoEP2478 has chaeta phenotype
Expression of pnrScer\UAS.cHa, under the control of Scer\GAL4tin.cBa, in a Df(3L)DocA background provides a moderate rescue of cardioblast formation. Coexpression of pnrScer\UAS.cHa and tinScer\UAS.cYa, under the control of Scer\GAL4tin.cBa, induces the formation of more cardioblasts than expression of pnrScer\UAS.cHa alone. When Doc2Scer\UAS.cRa is coexpressed with only pnrScer\UAS.cHa, or pnrScer\UAS.cHa and tinScer\UAS.cYa together, large numbers of extra cardioblasts are formed. Expression of all three transgenes results in double the amount of cardioblasts compared to wild type. Coexpression of Doc2Scer\UAS.cRa, tinScer\UAS.cYa, and pnrScer\UAS.cHa, under the control of Scer\GAL4twi.2PE, causes widespread ectopic cardioblast formation to a much greater extent than expression of Doc2Scer\UAS.cRa alone or in combination with tinScer\UAS.cYa plus pnrScer\UAS.cHa.
Flies expressing both pnrScer\UAS.cHa and ppoEP2478 under the control of Scer\GAL4ap-md544 in a pnrMD237/+ background show a reduction in thoracic bristles and an altered scutellum.
The addition of overexpressed pnrScer\UAS.cHa (when driven by Scer\GAL4pnr-MD237) suppresses the cleft phenotype of ChiE mutants.
The dorsocentral bristle phenotype seen in flies expressing pnrScer\UAS.cHa using Scer\GAL4pnr-MD237 is enhanced by Df(2L)ushrev18.
pnrUAS.cHa/Scer\GAL4Mef2.PR partially rescues pnr1
pnrUAS.cHa/Scer\GAL4tin.CΔ4 partially rescues pnr1
pnrUAS.cHa, Scer\GAL4twi.PG, Scer\GAL4how-24B partially rescues pnr1
pnrUAS.cHa, Scer\GAL4twi.PG, Scer\GAL4how-24B partially rescues pnrVX6
pnrUAS.cHa/Scer\GAL4Mef2.PR partially rescues pnrVX6
pnrUAS.cHa/Scer\GAL4tin.CΔ4 partially rescues pnrVX6
pnrUAS.cHa/Scer\GAL4how-24B partially rescues pnrVX6
pnrUAS.cHa/Scer\GAL4da.G32 partially rescues pnrVX6
Scer\GAL4zen.Kr.PF/pnrUAS.cHa partially rescues pnrVX6
pnrUAS.cHa, Scer\GAL4twi.PG, Scer\GAL4how-24B partially rescues pnrVX6
The increased arrest/fibrillation rate of pnr1/+ and pnrVX6/+ flies that is seen upon electrical pacing of the heart is partially rescued by expression of pnrScer\UAS.cHa under the control of Scer\GAL4Mef2.PR.
The increased arrest/fibrillation rate of pnr1/+ and pnrVX6/+ flies that is seen upon electrical pacing of the heart is partially rescued by expression of pnrScer\UAS.cHa under the control of Scer\GAL4tin.CΔ4 (rescue of the phenotype is seen in 1 and 3 week old flies, but not in 5 week old flies).
The increased arrest/fibrillation rate of pnr1/+ and pnrVX6/+ flies that is seen upon electrical pacing of the heart is partially rescued by expression of pnrScer\UAS.cHa under the simultaneous control of both Scer\GAL4twi.PG and Scer\GAL4how-24B (rescue of the phenotype is seen in 1 and 3 week old flies, but not in 5 week old flies).
The decreased lifespan of pnrVX6/+ flies can be partially rescued by expression of pnrScer\UAS.cHa under the simultaneous control of both Scer\GAL4twi.PG and Scer\GAL4how-24B.
The decreased lifespan of pnrVX6/+ flies can be partially rescued by expression of pnrScer\UAS.cHa under the control of either Scer\GAL4Mef2.PR or Scer\GAL4how-24B.
The arrhythmias and slow heart beats of pnrVX6/+ flies can be rescued by expression of pnrScer\UAS.cHa under the control of either Scer\GAL4Mef2.PR or Scer\GAL4tin.CΔ4.
The arrhythmias and slow heart beats of pnrVX6/+ flies can be rescued by expression of pnrScer\UAS.cHa under the simultaneous control of both Scer\GAL4twi.PG and Scer\GAL4how-24B.
When pnrScer\UAS.cHa is driven by Scer\GAL4how-24B and Scer\GAL4twi.PG in pnrVX6 embryos, 57% of embryos show a cardiac-specific tin expression that is restored close to wild-type levels. A similar level of rescue is seen when pnrScer\UAS.cHa is driven by Scer\GAL4da.G32. A small amount of rescue is also seen if pnrScer\UAS.cHa is driven by Scer\GAL4zen.Kr.PF in the mesoderm.
