UAS regulatory sequences drive expression of a full-length otp open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
Expression of otpUAS.ORF.GW.Tag:HA under the control of Scer\GAL4elav-C155 increases mitotic proliferation of neuroblasts and their daughter cells in the ventral nerve cord during late embryogenesis, compared to controls; expression under the control of Scer\GAL4vg.int2.1 in the larval wing disc has no effect on the number of neuroblasts or their daughter cells, compared to controls.
Expression of otpScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in partial female lethality at 18[o]C. The adult flies that are seen exhibit almost complete wing loss at 18[o]C.
Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA has increased occurrence of cell division | embryonic stage phenotype, enhanceable by Doc2UAS.cRa/ermUAS.ORF.GW.Tag:HA/tllUAS.cKa, Scer\GAL4elav-C155
otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of increased occurrence of cell division | embryonic stage phenotype of Doc2UAS.cRa, Scer\GAL4elav-C155
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, Scer\GAL4elav-C155 is an enhancer of increased occurrence of cell division | embryonic stage phenotype of Scer\GAL4elav-C155, tllUAS.cKa
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of increased occurrence of cell division | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA
Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA has larval ventral nerve cord | embryonic stage phenotype, enhanceable by Doc2UAS.cRa/ermUAS.ORF.GW.Tag:HA/tllUAS.cKa, Scer\GAL4elav-C155
Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA has neuroblast | embryonic stage phenotype, enhanceable by Doc2UAS.cRa/ermUAS.ORF.GW.Tag:HA/tllUAS.cKa, Scer\GAL4elav-C155
Scer\GAL4elav-C155, otpUAS.ORF.GW.Tag:HA has ganglion mother cell | embryonic stage phenotype, enhanceable by Doc2UAS.cRa/ermUAS.ORF.GW.Tag:HA/tllUAS.cKa, Scer\GAL4elav-C155
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4elav-C155, tllUAS.cKa
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4elav-C155, tllUAS.cKa
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA
otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Doc2UAS.cRa, Scer\GAL4elav-C155
otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of neuroblast | embryonic stage phenotype of Doc2UAS.cRa, Scer\GAL4elav-C155
otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Doc2UAS.cRa, Scer\GAL4elav-C155
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA, Scer\GAL4elav-C155 is an enhancer of ganglion mother cell | embryonic stage phenotype of Scer\GAL4elav-C155, tllUAS.cKa
Doc2UAS.cRa, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa, Scer\GAL4elav-C155 is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4elav-C155, ermUAS.ORF.GW.Tag:HA
Doc2UAS.cRa/otpUAS.ORF.GW.Tag:HA, Scer\GAL4vg.int2.1 is a suppressor | partially of wing disc phenotype of Scer\GAL4vg.int2.1, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa
Doc2UAS.cRa/otpUAS.ORF.GW.Tag:HA, Scer\GAL4vg.int2.1 is a suppressor | partially of neuroblast | ectopic | larval stage phenotype of Scer\GAL4vg.int2.1, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa
Doc2UAS.cRa/otpUAS.ORF.GW.Tag:HA, Scer\GAL4vg.int2.1 is a suppressor | partially of ganglion mother cell | ectopic | larval stage phenotype of Scer\GAL4vg.int2.1, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa
Doc2UAS.cRa/otpUAS.ORF.GW.Tag:HA, Scer\GAL4vg.int2.1 is a suppressor | partially of embryonic/larval neuron | ectopic | larval stage phenotype of Scer\GAL4vg.int2.1, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa
Doc2UAS.cRa/otpUAS.ORF.GW.Tag:HA, Scer\GAL4vg.int2.1 is a suppressor | partially of embryonic/larval glial cell | ectopic | larval stage phenotype of Scer\GAL4vg.int2.1, ermUAS.ORF.GW.Tag:HA, tllUAS.cKa
Doc2UAS.cRa, Scer\GAL4da.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | increased number | embryonic stage phenotype
Doc2UAS.cRa, Scer\GAL4wg.PU, ermUAS.ORF.GW.Tag:HA, otpUAS.ORF.GW.Tag:HA, tllUAS.cKa has neuroblast | embryonic stage phenotype
Expression of tllUAS.cKa, otpUAS.ORF.GW.Tag:HA, ermUAS.ORF.GW.Tag:HA and Doc2UAS.cRa under the control of Scer\GAL4elav-C155 significantly increases total cell number and the number of neuroblasts in the late embryonic ventral nerve cord, compared to controls; expression under the control of Scer\GAL4wg.PU results in a trend towards increased symmetric (abnormal) neuroblast division and significantly increased asymmetric (normal) daughter (ganglion mother cell) division as well as a significant increase in the number of cells in the NB5-6 lineage, compared to controls; expression under the control of Scer\GAL4pros.PMG significantly increases the number of neuroblasts and daughters in S-phase, with no effect on cell cycle speed, compared to controls; expression under the control of Scer\GAL4da.PU increases the number of mitotic cells, neuroblasts, daughters and neurons, and reduces the number of glia, with a thickening of the CNS, compared to controls.