FlyBase Allele Report: Dmel\Pen[CASB-.UASp]

General Information

Symbol

Dmel\Pen^CASB-.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0300967

Feature type

allele

Associated gene

Dmel\Pen

Associated Insertion(s)

Carried in Construct

P{UASp-Pen.CASB-}

Key Links

Transgenic product class

missense_variant

(Gorjanacz et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Gorjanacz et al., 2006)

Mutagen

in vitro construct

(Gorjanacz et al., 2006)

Progenitor genotype

Carried in construct

P{UASp-Pen.CASB-}

Cytology

Description

UASp regulatory sequences drive expression of mutated Pen cDNA sequence, which contains mutations in the CAS-binding domain (residues 459-464 have been mutated from GLDKLE to AAAAAA).

(Gorjanacz et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Pen

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RP13, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RX13, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^c02473, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e02657, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-enhancer of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e03750, Pen^D14

(Virágh et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RP13, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RX13, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^c02473, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e02657, Pen^D14

(Virágh et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^e03750, Pen^D14

(Virágh et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^c747/Pen^CASB-.UASp fails to rescue Pen^mbmB

(Serway et al., 2020)

Scer\GAL4^{VP16.nanos.UTR}/Pen^CASB-.UASp fails to rescue Pen^D14

(Gorjanacz et al., 2006)

Comments

Expression of Pen^{CASB-.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the low rate of egg laying in Pen^D14 females. None of the eggs that are laid hatch. Ring canals in the ovary are occluded by actin.

(Gorjanacz et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pen^{CASB-.Scer\UAS.P\T}

Pen^CASB-.UASp

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close