FlyBase Allele Report: Dmel\mahe[UAS.Tag:HA]

General Information

Symbol

Dmel\mahe^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0317103

Feature type

allele

Associated gene

Dmel\mahe

Associated Insertion(s)

Carried in Construct

P{UAS-mahe.HA}

Key Links

Transgenic product class

wild_type

(Surabhi et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Surabhi et al., 2015)

Mutagen

in vitro construct

(Surabhi et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-mahe.HA}

Cytology

Description

UASt regulatory sequences drive expression of mahe tagged with Tag:HA.

(Surabhi et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mahe

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^ey.PU

(Surabhi et al., 2015)

salivary gland, with Scer\GAL4^ey.PU

(Surabhi et al., 2015)

salivary gland, with Scer\GAL4^ptc.PU

(Surabhi et al., 2015)

scutellar bristle, with Scer\GAL4^ptc.PU

(Surabhi et al., 2015)

wing, with Scer\GAL4^ap.PU

(Surabhi et al., 2015)

wing margin, with Scer\GAL4^ptc.PU

(Surabhi et al., 2015)

Detailed Description

Statement

Reference

Expression of mahe^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PU results in a massive reduction in adult eye size in both male and female flies. The larval eye-antennal discs are also significantly smaller than those of controls.

Expression of mahe^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ptc.PU results in an enormous decrease in the size of the salivary gland with 100% penetrance. The salivary gland nuclei are much smaller than in controls. Notching is seen at the wing margin, the distance between the L3 and L4 veins is reduced and there is loss of sensory bristles in the scutellum. Suppression of apoptosis does not suppress this loss of bristles.

Expression of mahe^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^bbg-C96 has no visible effect on the wing margin.

Expression of mahe^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ap.PU results in blistered and notched wings.

(Surabhi et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, mahe^UAS.Tag:HA has visible phenotype, enhanceable by Df(1)N-54l9/+

(Surabhi et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, mahe^UAS.Tag:HA has visible phenotype, suppressible | partially by dx¹⁵²/dx[+]

(Surabhi et al., 2015)

Enhancer of

Statement

Reference

mahe^UAS.Tag:HA/Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Surabhi et al., 2015)

Suppressor of

Statement

Reference

mahe^UAS.Tag:HA, Scer\GAL4^ey.PU is a suppressor of hyperplasia | larval stage phenotype of N^ICN.UAS, Scer\GAL4^ey.PU

(Surabhi et al., 2015)

Other

Statement

Reference

Scer\GAL4^bbg-C96, dx^UAS.cMa, mahe^UAS.Tag:HA has visible phenotype

(Surabhi et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, mahe^UAS.Tag:HA has eye phenotype, enhanceable by Df(1)N-54l9/+

(Surabhi et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, mahe^UAS.Tag:HA has eye phenotype, suppressible | partially by dx¹⁵²/dx[+]

(Surabhi et al., 2015)

Enhancer of

Statement

Reference

mahe^UAS.Tag:HA/Scer\GAL4^bbg-C96 is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Surabhi et al., 2015)

Suppressor of

Statement

Reference

mahe^UAS.Tag:HA, Scer\GAL4^ey.PU is a suppressor of eye disc | larval stage phenotype of N^ICN.UAS, Scer\GAL4^ey.PU

(Surabhi et al., 2015)

Other

Statement

Reference

Scer\GAL4^bbg-C96, dx^UAS.cMa, mahe^UAS.Tag:HA has wing margin phenotype

(Surabhi et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Df(1)N-54l9 significantly enhances the reduced eye phenotype seen when mahe^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey.PU with 100% penetrance.

One copy of dx¹⁵² marginally suppresses the reduced eye phenotype seen when mahe^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey.PU in 32% of eyes examined.

Expression of mahe^{Scer\UAS.T:Ivir\HA1} partially suppresses the highly proliferated eye tissue phenotype seen when N^ICN.Scer\UAS is expressed under the control of Scer\GAL4^ey.PU.

Co-expression of mahe^{Scer\UAS.T:Ivir\HA1} and dx^Scer\UAS.cMa under the control of Scer\GAL4^bbg-C96 results in wing margin nicking.

(Surabhi et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mahe^{Scer\UAS.T:Ivir\HA1}

mahe^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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