FlyBase Allele Report: Dmel\dx[UAS.cMa]

General Information

Symbol

Dmel\dx^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Matsuno

FlyBase ID

FBal0102743

Feature type

allele

Associated gene

Dmel\dx

Associated Insertion(s)

Carried in Construct

P{UAS-DxA89}

Also Known As

UAS-dx, UASdx, UAS-deltex

Key Links

Transgenic product class

wild_type

(Matsuno et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Matsuno et al., 2002)

Mutagen

in vitro construct

(Matsuno et al., 2002, Helms et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-DxA89}

Cytology

Description

UASt regulatory sequences drive expression of dx.

(Matsuno et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal lateral adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

anterior crossvein, with Scer\GAL4^ptc-559.1

(Sakata, 2004)

leg, with Scer\GAL4^ap-md544

(Capilla et al., 2012)

leg joint | ectopic, with Scer\GAL4^ap-md544

(Capilla et al., 2012)

microchaeta & mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Moores et al., 2008)

sensory mother cell | ectopic, with Scer\GAL4^ptc-559.1

(Matsuno et al., 2002)

wing disc | ventral, with Scer\GAL4^ptc-559.1

(Mazaleyrat et al., 2003)

wing margin | ectopic, with Scer\GAL4^ptc-559.1

(Matsuno et al., 2002)

wing margin bristle, with Scer\GAL4^bbg-C96

(Surabhi et al., 2015)

wing margin bristle | ectopic, with Scer\GAL4^ptc-559.1

(Matsuno et al., 2002)

Detailed Description

Statement

Reference

Expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^{E(spl)m6-BFM.PF} leads to significantly increased numbers of abdominal lateral adult muscle precursor cells in third instar larvae, as compared to controls.

(Aradhya et al., 2015)

Expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^bbg-C96 results in mild irregularities of bristles at the wing margin.

(Surabhi et al., 2015)

Expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 results in ectopic joints in the leg.

(Capilla et al., 2012)

Expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^pnr-MD237 at 19[o]C results in a large reduction in the number of microchaetae on the notum (in the region of Scer\GAL4^pnr-MD237 expression), with many remaining bristles developing abnormally as socket-only, double, triple or groups of five or more bristles.

(Moores et al., 2008)

Clones in the wing disc that express dx^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PP show additional cell proliferation compared to wild type.

(Koelzer and Klein, 2006)

Expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^ptc-559.1 results in a loss of the anterior crossvein in the wing.

(Sakata, 2004)

When dx^Scer\UAS.cMa is driven by Scer\GAL4^ptc-559.1 leads to an enlarged ventral compartment in the wing disc.

(Mazaleyrat et al., 2003)

When dx^Scer\UAS.cMa is driven by Scer\GAL4^ptc-559.1 a secondary wing-margin like structure is induced in the wing. Tis structure includes ectopic outgrowth and ectopic bristle formation.

(Matsuno et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, dx^UAS.cMa has visible phenotype, enhanceable by Hip1^UAS.cMa, Scer\GAL4^pnr-MD237

(Moores et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^{E(spl)m6-BFM.PF}, dx^UAS.cMa has increased cell number | third instar larval stage phenotype, suppressible by krz^UAS.cRa/Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Scer\GAL4^pnr-MD237, dx^UAS.cMa has visible phenotype, suppressible | partially by Hip1^ΔANTH.UAS, Scer\GAL4^pnr-MD237

(Moores et al., 2008)

Scer\GAL4^αTub84B.PP, dx^UAS.cMa has increased cell number | somatic clone phenotype, suppressible by Su(H)^del47

(Koelzer and Klein, 2006)

Suppressor of

Statement

Reference

dx^UAS.cMa/Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^{E(spl)m6-BFM.PF}, krz^UAS.cRa

(Aradhya et al., 2015)

dx^UAS.cMa, Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of decreased cell number | third instar larval stage phenotype of Pten^UAS.cHa, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/dx^UAS.cMa is a non-suppressor of visible phenotype of dsh¹

(Capilla et al., 2012)

Other

Statement

Reference

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}, dx^UAS.cMa has increased cell number | third instar larval stage phenotype

(Aradhya et al., 2015)

Scer\GAL4^bbg-C96, dx^UAS.cMa, mahe^UAS.Tag:HA has visible phenotype

(Surabhi et al., 2015)

Nedd4^C974FS, Scer\GAL4^ptc-559.1, dx^UAS.cMa has visible phenotype

(Sakata, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, dx^UAS.cMa has microchaeta & mesothoracic tergum phenotype, enhanceable by Hip1^UAS.cMa, Scer\GAL4^pnr-MD237

(Moores et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^{E(spl)m6-BFM.PF}, dx^UAS.cMa has abdominal lateral adult muscle precursor cell | third instar larval stage phenotype, suppressible by krz^UAS.cRa/Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Scer\GAL4^pnr-MD237, dx^UAS.cMa has microchaeta & mesothoracic tergum phenotype, suppressible | partially by Hip1^ΔANTH.UAS, Scer\GAL4^pnr-MD237

(Moores et al., 2008)

Scer\GAL4^ptc-559.1, dx^UAS.cMa has wing disc | ventral phenotype, suppressible by Scer\GAL4^ptc-559.1/Su(dx)^UAS.cCa

(Mazaleyrat et al., 2003)

NOT Enhancer of

Statement

Reference

Scer\GAL4^bbg-C96/dx^UAS.cMa is a non-enhancer of wing phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Suppressor of

Statement

Reference

dx^UAS.cMa, Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of abdominal lateral adult muscle precursor cell | third instar larval stage phenotype of Pten^UAS.cHa, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

dx^UAS.cMa/Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of abdominal lateral adult muscle precursor cell | third instar larval stage phenotype of Scer\GAL4^{E(spl)m6-BFM.PF}, krz^UAS.cRa

(Aradhya et al., 2015)

Scer\GAL4^ptc-559.1/dx^UAS.cMa is a suppressor of wing disc phenotype of Scer\GAL4^ptc-559.1, Su(dx)^UAS.cCa

(Mazaleyrat et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/dx^UAS.cMa is a non-suppressor of leg joint | ectopic phenotype of dsh¹

(Capilla et al., 2012)

Scer\GAL4^ap-md544/dx^UAS.cMa is a non-suppressor of leg phenotype of dsh¹

(Capilla et al., 2012)

Scer\GAL4^bbg-C96/dx^UAS.cMa is a non-suppressor of wing phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Other

Statement

Reference

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}, dx^UAS.cMa has abdominal lateral adult muscle precursor cell | third instar larval stage phenotype

(Aradhya et al., 2015)

Scer\GAL4^bbg-C96, dx^UAS.cMa, mahe^UAS.Tag:HA has wing margin phenotype

(Surabhi et al., 2015)

Nedd4^C974FS, Scer\GAL4^ptc-559.1, dx^UAS.cMa has wing phenotype

(Sakata, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dx^Scer\UAS.cMa and krz^Scer\UAS.cRa under the control of Scer\GAL4^{E(spl)m6-BFM.PF} suppresses the increased number of abdominal lateral adult muscle precursor cells in third instar larvae observed upon expression of either dx^Scer\UAS.cMa or krz^Scer\UAS.cRa alone.

The co-expression of dx^Scer\UAS.cMa and InR^{R418P.Scer\UAS} under the control of Scer\GAL4^{E(spl)m6-BFM.PF} results in increased numbers of abdominal lateral adult muscle precursor cells present in third instar larvae, as compared to controls.

The co-expression of dx^Scer\UAS.cMa suppresses the decreased number of abdominal lateral adult muscle precursor cells present in third instar larvae expressing Pten^Scer\UAS.cHa under the control of Scer\GAL4^{E(spl)m6-BFM.PF}.

(Aradhya et al., 2015)

Co-expression of mahe^{Scer\UAS.T:Ivir\HA1} and dx^Scer\UAS.cMa under the control of Scer\GAL4^bbg-C96 results in wing margin nicking.

(Surabhi et al., 2015)

Expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 has no effect on the ectopic leg joint phenotype of dsh¹ flies.

(Capilla et al., 2012)

The phenotype of reduced microchaetae on the notum which is caused by expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^pnr-MD237 at 19[o]C is enhanced by co-expression of Hip1^Scer\UAS.cMa, such that microchaetae are almost completely eliminated in the region of Scer\GAL4^pnr-MD237 expression.

The phenotype of reduced microchaetae on the notum which is caused by expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^pnr-MD237 at 19[o]C is partially suppressed by co-expression of Hip1^{ΔANTH.Scer\UAS}; bristle number is increased and the number of abnormal bristles is reduced.

(Moores et al., 2008)

Unlike expression in clones with a wild-type background, expression of dx^Scer\UAS.cMa (driven by Scer\GAL4^αTub84B.PP) in Su(H)^del47 mutant clones does not result in increased cell proliferation.

(Koelzer and Klein, 2006)

The wing phenotype seen in flies co-expressing Nedd4^GS13178 and dx^Scer\UAS.cMa under the control of Scer\GAL4^ptc-559.1 resembles that seen in flies expressing Nedd4^GS13178 alone under the control of Scer\GAL4^ptc-559.1. Co-expression of Nedd4^C974FS enhances the wing phenotype caused by expression of dx^Scer\UAS.cMa under the control of Scer\GAL4^ptc-559.1; ectopic wing margin is formed.

(Sakata, 2004)

Has no effect on the wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies.

(Helms et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dx^UAS.cMa is rescued by Scer\GAL4^ptc-559.1/dx^ΔPRM.UAS

(Matsuno et al., 2002)

Rescues

Scer\GAL4^ptc-559.1/dx^UAS.cMa rescues dx^ΔPRM.UAS

(Matsuno et al., 2002)

Partially rescues

dx^UAS.cMa/Scer\GAL4^hs.PB partially rescues dx²⁴

(Matsuno et al., 2002)

Comments

The combination of dx^{ΔPRM.Scer\UAS} and dx^Scer\UAS.cMa (driven by Scer\GAL4^ptc-559.1) suppresses the phenotypes seen with either construct alone. The addition of Scer\GAL4^hs.PB (driven by Scer\GAL4^hs.PB) can rescue the dx²⁴ wing phenotype, completely rescuing 18% of the time.

(Matsuno et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dx^Scer\UAS.cMa

dx^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Matsuno

(Helms et al., 1999)

Secondary FlyBase IDs

References (13)

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